Guangdong Entomological Institute

Guangzhou, China

Guangdong Entomological Institute

Guangzhou, China
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Guangdong Entomological Institute | Date: 2014-12-02

A method for artificial cultivation of Ophiocordyceps sinensis fruiting bodies. The method comprises: inoculating Ophiocordyceps sinensis into a sterile rice medium, cultivating at 9-13 C. for 40-60 days, after the medium is covered with mycelia, performing low-temperature induction at 1-8 C. for 60-80 days to develop a fruiting body primordium, and transferring the cultivation to 11-16 C. till harvest of the fruiting bodies. The method provided by the present invention requires no low-oxygen environment, which can reduce cultivation cost; it only needs 3-4 months from induction to harvest of fruiting bodies; the rice medium for use has a low cost, which is suitable for commercial cultivation of Ophiocordyceps sinensis fruiting bodies.

Agency: European Commission | Branch: FP7 | Program: CP-FP-SICA | Phase: KBBE.2012.2.3-05 | Award Amount: 3.84M | Year: 2013

Food security is a global challenge. Within the overall increased demand for food, and particularly meat production, there is also an urgent need to increase supply of protein from sustainable sources. The principle objective of the international and multidisciplinary PROteINSECT consortium is to facilitate the exploitation of insects as an alternative protein source for animal and human nutrition. Advances have been made in rearing of insects for incorporation in animal feed in countries including China and Mali. The consortium brings together expertise in these countries together with European insect breeders and feed production companies in order to optimise systems and set up pilot scale production facilities in the EU. The project will demonstrate the feasibility of the use of insect-derived proteins in animal feed through trials with fish, poultry and pigs. Quality and safety along the food chain from insect protein itself, to incorporation in feed and ultimately human consumption of insect-protein reared livestock, will be evaluated. The use of waste streams that focus on animal rather than plant material for insect rearing will be examined. To optimise the economic viability of the use of insect proteins, uses for the residual flows from the production system will be determined. Life cycle analyses will enable the design of optimised and sustainable production systems suitable for adoption in both ICPC and European countries. Key to uptake is ensuring that a regulatory framework is in place and this will be encouraged by the preparation of a White Paper following consultation with key stakeholders, experts and consumers. PROteINSECT will build a pro-insect platform in Europe to encourage adoption of sustainable protein production technologies in order to reduce the reliance of the feed industry on plant/fish derived proteins in the short term, and promote the acceptance of insect protein as a direct component of human food in the longer term.

Ai H.,Sun Yat Sen University | Ai H.,Guangdong Entomological Institute | Yan X.,Guangdong Entomological Institute | Han R.,Guangdong Entomological Institute
Journal of Invertebrate Pathology | Year: 2012

Populations of Apis mellifera and Apis cerana in China were surveyed for seven bee viruses: acute bee paralysis virus (ABPV), black queen cell virus (BQCV), chronic bee paralysis virus (CBPV), deformed wing virus (DWV), Kashmir bee virus (KBV), sacbrood virus (SBV), and Isreal acute paralysis virus (IAPV). No KBV was detected from any samples of the two species. In A. mellifera, DWV was the most prevalent virus, but in A. cerana, SBV was the dominant. Simultaneous multiple infections of viruses were common in both species. This is the first report of detection of IAPV and CBPV in A. cerana. © 2011 Elsevier Inc.

Zhang L.B.,Guangdong Entomological Institute
Dong wu xue yan jiu = Zoological research / "Dong wu xue yan jiu" bian ji wei yuan hui bian ji | Year: 2010

One male bat was collected in a cave in Cuibi Hill (N: 21 degree 53', E: 101 degree 18', H: 683 m a.s.l.), Menglun Town, Mengla County, Yunnan Province, on November 26, 2006. This bat is of medium body size, with 60.7 mm forearm and 21.4 g body mass. Its ears are large ovals and joined medially to the forehead at about 15% of the height of inner margin. The tragus of each ear is slender and distinctly bifid. The noseleaf is simple, and the posterior noseleaf is oval with obtuse tip and convex sides, a significant longitudinal ridge laying middle, which connects to intermediate noseleaf at the base. Intermediate noseleaf presents triangle with a W-shaped tip. Frontal noseleaf is horseshoe shape and attached directly to the muzzle. The tail is absent, and the second finger of each wing has only one phalanx. There are no upper incisors, Pm3 and Pm3. The upper canine has an anterior and a large posterior basal cusps. This bat is identified as lesser false vampire, Megaderma spasma, which is a new record of China. Its external and craniodental measurements were presented and compared with those of M. lyra. The specimen is preserved in Guangdong Entomological Institute.

Li Z.Q.,Guangdong Entomological Institute
Journal of insect science (Online) | Year: 2013

Cellulose digestion in termites (Isoptera) is highly important for ecological reasons and applications in biofuel conversion. The speciose Termitidae family has lost flagellates in the hindgut and developed diverse feeding habits. To address the response of cellulase activity to the differentiation of feeding habits, a comparative study of the activity and distribution of composite cellulases, endo-β-1,4-glucanase, and β-glucosidase was performed in seven common flagellate-free termites with three feeding habits: the humus-feeding termites Sinocapritermes mushae (Oshima et Maki), Malaysiocapritermes zhangfengensis Zhu, Yang et Huang and Pericapritermes jiangtsekiangensis (Kemner); the fungus-growing termites Macrotermes barneyi Light and Odontotermes formosanus (Shiraki); and the wood-feeding termites Nasutitermes parvonasutus (Shiraki) and Havilanditermes orthonasus (Tsai et Chen). The results showed that in diverse feeding groups, the wood-feeding group had the highest total composite cellulase and endo-β-1,4-glucanase activities, while the fungus-growing group had the highest β-glucosidase activity. In terms of the distribution of cellulase activity in the alimentary canals, the cellulase activities in wood-feeding termites were concentrated in the midgut, but there was no significant difference between all gut segments in humus-feeding termites. As for the fungus-growing termites, the main site of composite cellulase activity was in the midgut. The endo-β-1,4-glucanase activity was restricted to the midgut, but the primary site of β-glucosidase activity was in the foregut and the midgut (Mac. barneyi). The functions of the gut segments apparently differentiated between feeding groups. The results suggest that the differentiation of feeding habits in flagellate-free termites was characterized by the distribution of cellulases in the gut rather than by variations in cellulase activity.

Zhong J.-H.,Guangdong entomological institute
Sociobiology | Year: 2011

The genus Coptotermes is in serious need of revision in China. The Asian subterranean termite, C. gestroi, is an economically important structural pest. It was proposed that C. yaxianensis Li and C. obliquus Xia et He were two new junior synonyms of C. gestroi. C. gestroi was distributed in Yunnan province and Taiwan, and now C. gestroi has been brought into new geographical regions including Sanya city and Changjiang county in the Hainan province of China.

Liu Z.X.,Guangdong Entomological Institute
Dong wu xue yan jiu = Zoological research / "Dong wu xue yan jiu" bian ji wei yuan hui bian ji | Year: 2013

Chinese chiropterologists have made significant improvements into research on bat taxonomy and distribution. Overall, scholars recorded 6 new species of bats, alongside 11 species recorded species in the Chinese Mainland and 4 new bat species of Murina in Taiwan. Chinese chiropterologists intensively cooperated with the international experts on bats, and adopted several new, multidisciplinary methods to carry out their studies. Likewise, in China, an increased awareness of bat conservation has been growing. While publications on Chiroptera are continuing to increase increased in China, the methodology of these studies remains to be further developed in hopes of revealing the new and cryptic bat species. Considering the vast territory of China and the migrational habit of bats, we expect that with more refined methodology, more new species of bats and their distributions may be uncovered in the near future. Concurrently, it is important to reexamine the known species by the new taxonomic methods and fauna analysis through which the distribution and subdivision of bats can be updated. Additionally, an international platform for exchanging information of bats needs to be established to enhance the academic cooperation for bat researches. It is highly possible that China will soon become an important research center on taxonomy, distribution, phylogenetics and diversity evolution of Chiroptera, especially as Chinese researchers continues create new knowledge for bats at the α, β and γ taxonomic levels.

An artificial feeding method at low altitude for a host insect ghost moth of Ophiocordyceps sinensis. This method mainly includes: selecting an appropriate disinfectant for performing surface disinfection on ghost moth eggs, and then placing the disinfected eggs in sterile humus; feeding ghost moth larvae with carrots, disinfecting and detecting the fed carrots, and culturing the larvae till male and female pupae are obtained; identifying male and female pupae of the ghost moths, placing them at different culture temperatures, wherein the culture temperature for the male pupae is 2-6 C. lower than that of the female pupae, resulting synchronously emerged male and female adults; placing the emerged male and female adults in an adult cage with simulated plants for mating and staying of the adults, maintaining a humidity over 80%, and employing 50-100 Lux shimmer for facilitating activity and mating of the adults, thus improving fertilization opportunity and hatchability of eggs when the adults after mating lay eggs.

Guangdong Entomological Institute | Date: 2014-04-04

The invention discloses an artificial pairing and breeding method of Platysternon megacephalum. The method comprises the selection of species sources, domestication before pairing, setting of a breeding pond, artificial pairing, collection and hatching of turtle eggs. The method fully considers the ecological and biological characteristics of the Platysternon megacephalum, including the behavior, feeding habit, habitat, and breeding ecology. The main problems in artificial pairing and captive breeding of the Platysternon megacephalum can be effectively solved, including the breeding turtle selection, pairing, pairing stage selection, sex ratio, density, artificial food, setting of the reproduction and hatching environments, the occurrence of disabilities and fatal events during the pairing and mating process is significantly reduced; the egg laying rate of female turtles and the hatching rate of turtle eggs are both significantly improved. Artificial high-efficiency breeding of the Playsternon megacephalum is realized, and healthy turtle hatchlings can be obtained.

Guangdong Entomological Institute | Date: 2013-07-16

The present invention discloses a rapid diagnosis method of citrus huanglongbing. In the present invention, the accuracy of diagnosis of citrus huanglongbing is significantly improved in the following manners: eliminating the interference of residual starch by effectively removing the residual starch contained in the leaf; eliminating the interference of chlorophyll by effectively removing chlorophyll; and directly developing the color on the leaf rather than mixing and grinding the leaf in water, so as to avoid the problem that the accuracy of color development reaction conducted by mixing the leaf with water is liable to be interfered since the starch is insoluble in water. Therefore a rapid diagnosis of the citrus huanglongbing with much higher accuracy is achieved, which provides effective detection means for the control of the citrus huanglongbing and is beneficial for the control of the citrus huanglongbing.

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