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Qi P.,Guangdong Communication Polytechnic | Li Y.,Jinan University | Zhong J.,Jinan University
Guangdian Gongcheng/Opto-Electronic Engineering | Year: 2015

The Surface Plasmon Resonance (SPR) sensor is widely applied in the immune reaction detection of biomolecules. For the self-developed angle scanning surface plasmon resonance sensor, the kinetics detection and kinetics data analysis software system were developed according to Langumair model, and the data processing method of polynomial fitting combined with centroid method was proposed. The established method could effectively inhibit the interference on SPR resonance angle detection by noise, ambient temperature and mechanical vibration in the process of detection. The immune reaction kinetics of mice antigen and antibody was detected. Its association rate constant ka≈2.05×104 M-s- and issociation rate constant kb≈0.005 s-. The feasibility of the established device and data processing method was confirmed. This method could effectively improve the accuracy and capacity of resisting disturbance of angle scanning surface plasmon resonance sensor, and it is worthy to be applied and promoted. surface plasmon ©, 2015, Chinese Academy of Sciences. All right reserved.

Li Y.,Jinan University | Ma X.,Jinan University | Zhao M.,Jinan University | Qi P.,Guangdong Communication Polytechnic | Zhong J.,Jinan University
PLoS ONE | Year: 2014

Coumaphos is a common organophosphorus pesticide used in agricultural products. It is harmful to human health and has a strictly stipulated maximum residue limit (MRL) on fruits and vegetables. Currently existing methods for detection are complex in execution, require expensive tools and are time consuming and labor intensive. The surface plasmon resonance method has been widely used in biomedicine and many other fields. This study discusses a detection method based on surface plasmon resonance in organophosphorus pesticide residues. As an alternative solution, this study proposes a method to detect Coumaphos. The method, which is based on surface plasmon resonance (SPR) and immune reaction, belongs to the suppression method. A group of samples of Coumaphos was detected by this method. The concentrations of Coumaphos in the samples were 0 μg/L, 50 μg/L, 100 μg/L, 300 μg/L, 500 μg/L, 1000 μg/L, 3000 μg/L and 5000 μg/L, respectively. Through detecting a group of samples, the process of kinetic reactions was analyzed and the corresponding standard curve was obtained. The sensibility is less than 25 μg/L, conforming to the standard of the MRL of Coumaphos stipulated by China. This method is label-free, using an unpurified single antibody only and can continuously test at least 80 groups of samples continuously. It has high sensitivity and specificity. The required equipments are simple, environmental friendly and easy to control. So this method is promised for a large number of samples quick detection on spot and for application prospects. © 2014 Li et al.

Liu S.,South China University of Technology | Liu S.,South China Normal University | Qi D.,South China University of Technology | Meng J.,Guangdong Communication Polytechnic | Liu B.,Guangdong University of Technology
International Journal of Digital Content Technology and its Applications | Year: 2012

Wireless sensor networks (WSNs) are usually deployed in the non-controlled environment without maintenance, facing the danger of information disclosure, tampering, replay attacks, denial of service and other threats, it makes collaboration among WSN nodes becomes more and more vital. Some common trust collaboration model is analyzed and summarized in this paper firstly, after studying the collaboration of the ant colony system, the trust model based on Biologically-inspired Trust and Reputation Model (BTRM) used for wireless sensor networks is put forward, also the features and architecture of the simulation system is proposed. This paper describes BTRM theory and the design and implementation of simulation system in detail. The simulation shows the output average path length, the average satisfaction, the average deviation and so on is more satisfied with the improvements.

Cai P.,Guangdong Communication Polytechnic | Tu J.,Guangdong Communication Polytechnic
Wuhan Ligong Daxue Xuebao (Jiaotong Kexue Yu Gongcheng Ban)/Journal of Wuhan University of Technology (Transportation Science and Engineering) | Year: 2014

For the effective deployment of empty container in port, in order to improve the utilization efficiency of container leasing company, and to obtain good economic benefit, this paper constructs the three game model of empty containers scheduling in port, and carried on the empirical analysis to obtain: under the unified dispatching of ports (empty container has allocated between ports) and under separate scheduling (empty container has allocated between ports under the company headquarters to establish between the ports of transshipment price) of the two kinds of scheduling policy has the same operation results in five kinds of scheduling policy. Dispatching them in five strategy is optimal, which can get the company overall minimum container stock, the overall effect of maximum profit.

Wang G.,Guangdong Communication Polytechnic | Sun Y.,Guangdong Communication Polytechnic
Wuhan Ligong Daxue Xuebao (Jiaotong Kexue Yu Gongcheng Ban)/Journal of Wuhan University of Technology (Transportation Science and Engineering) | Year: 2013

An inspection system is offered for stay cables by magnetic leakage field, due for which the nondestructive test only. The measured results have showed that the MLF signals for cables fault is very unclear with too many noisy signals, so the analytical method of smooth process, wavelet difference transfinite values filter and BP neural network pattern recognition is applied for measured data to obtain the distinct character signals. The simulation results showed that the default signals have a distinct character by that filter process, so that the credible proof will be given about detection and analysis to the broken wires for the stay cable.

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