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Gao J.,Guangdong Agriculture Industry Business Polytechnic College | Li Y.,CAS Guangzhou Institute of Energy Conversion
Advanced Materials Research | Year: 2011

AC algorithm of blind sources has been applied to the identification of engine noise sources. However, the testing platform based on the blind separation theory is difficult to actualize some experiments and obtain data in the real environment, or to obtain these requires high cost. Therefore, this paper proposes a new approach to construct an experimental testing platform-Sinmulink Model so as to identify engine surface noise sources. This model is applied to the test of the experimental data. These experiments demonstrate that Simulink model is efficient to identify engine noise source and the testing data accord with that made by the former platform. It is obvious that Simulink model, to some degree, can be used to replace engine surface noise sources testing platform. © 2011 Trans Tech Publications.


PubMed | Biocontrol and Guangdong Agriculture Industry Business Polytechnic College
Type: | Journal: ZooKeys | Year: 2016

Five species of the genus


Xie X.-F.,South China Agricultural University | Xie X.-F.,Guangdong Agriculture Industry Business Polytechnic College | Yan H.-F.,CAS South China Botanical Garden | Wang F.-Y.,Shanghai Chenshan Botanical Garden | And 3 more authors.
Journal of Systematics and Evolution | Year: 2012

To investigate the mechanisms responsible for the high diversity in central and adjacent southwestern China, we inferred the phylogeographic history of Primula ovalifolia from chloroplast DNA data. One hundred and thirty five individuals from 13 natural populations (including one Primula tardiflora population) were analyzed. A total of 23 haplotypes were identified. Most of them were exclusive to a single population. Strong phylogeography structure was detected, with NST (0.936) significantly higher than GST (0.784). Phylogenetic analysis showed that all haplotypes were clustered into three lineages (clades I, II, and III). High genetic diversity was revealed, possibly due to the interglacial contraction and glacial expansion. The three identified lineages may have undergone different historical processes after the mid-Pleistocene, due to their early divergent time. Multiple refugia were inferred for the three lineages. Pleistocene climatic oscillations, combined with the complex local topography, were responsible for the strong phylogeographic pattern of P. ovalifolia. Our analysis supports the hypothesis that the central and adjacent southwestern region of China was an important refugium for the survival, persistence, and further speciation of most East Asia flora, which has led to high species diversity in this region. © 2012 Institute of Botany, Chinese Academy of Sciences.


Yan H.-F.,CAS South China Botanical Garden | Liu Y.-J.,South China Agricultural University | Xie X.-F.,Guangdong Agriculture Industry Business Polytechnic College | Zhang C.-Y.,South China Agricultural University | And 3 more authors.
PLoS ONE | Year: 2015

The genus Primula is extremely diverse in the east Himalaya-Hengduan Mountains (HHM) in China as a result of rapid radiation. In order to overcome the difficulty of morphological classification of this genus, we surveyed three plastid regions (rbcL, matK, and trnH-psbA) and two nuclear markers (ITS and ITS2) from 227 accessions representing 66 Primula species across 18 sections, to assess their discriminatory power as barcodes. We found that ITS alone or combined with plastid regions showed the best discrimination across different infrageneric ranks and at species level. We suggest rbcL + matK + ITS as the first choice at present to barcode Primula plants. Although the present barcoding combination performed poorly in many closely related species of Primula, it still provided many new insights into current Primula taxonomy, such as the underlying presence of cryptic species, and several potential improper taxonomic treatments. DNA barcoding is one useful technique in the integrative taxonomy of the genus Primula, but it still requires further efforts to improve its effectiveness in some taxonomically challenging groups. © 2015 Yan et al.


Tang H.,Guangdong Agriculture Industry Business Polytechnic College | Tang H.,CAS South China Botanical Garden | Deng Y.,CAS South China Botanical Garden
Phytotaxa | Year: 2016

Justicia latiflora Hemsl. in Forbes & Hemsley (1890: 245) is a species endemic to China occurring in Chongqing, Guangxi, Guizhou, Hubei, Hunan and Yunnan (Hu 2002, Hu et al. 2011). Later, it was transferred to Mananthes Bremekamp (1948: 59) as M. latiflora (Hemsl.) C.Y.Wu & C.C. Hu in Hu (2002: 295). Mananthes is one of the genera separated from Justicia Linnaeus (1753: 15) by Bremekamp to accommodate those Asian species having terminal spikes and it now is treated as a synonym of Justicia (Scotland & Vollesen 2000, Hu et al. 2011). This species differs from differ from those Chinese species formerly included in Mananthes in its bracts being purplish towards the apex and longer than the calyces, with corollas ca. 2 cm long. Three collections (Henry 3412, 3412A, 3412B) were cited from K in the protologue. Since no holotype was indicated they all are syntypes according to Art. 9.5 (ICN, McNeill et al. 2012). According to Art. 9.12, the lectotype must be chosen from those syntypes in first place. Comparing the three syntypes at K with the original description, we found that all are a match and candidates for serving as a lectotype. Since Henry 3412 (K000884035) is the only specimen with open corollas, a feature described in the protologue, it is here selected as the lectotype. © 2016 Magnolia Press.


PubMed | South China Agricultural University, Guangdong Agriculture Industry Business Polytechnic College and CAS South China Botanical Garden
Type: Journal Article | Journal: PloS one | Year: 2015

The genus Primula is extremely diverse in the east Himalaya-Hengduan Mountains (HHM) in China as a result of rapid radiation. In order to overcome the difficulty of morphological classification of this genus, we surveyed three plastid regions (rbcL, matK, and trnH-psbA) and two nuclear markers (ITS and ITS2) from 227 accessions representing 66 Primula species across 18 sections, to assess their discriminatory power as barcodes. We found that ITS alone or combined with plastid regions showed the best discrimination across different infrageneric ranks and at species level. We suggest rbcL + matK + ITS as the first choice at present to barcode Primula plants. Although the present barcoding combination performed poorly in many closely related species of Primula, it still provided many new insights into current Primula taxonomy, such as the underlying presence of cryptic species, and several potential improper taxonomic treatments. DNA barcoding is one useful technique in the integrative taxonomy of the genus Primula, but it still requires further efforts to improve its effectiveness in some taxonomically challenging groups.


Ye M.,Guangdong Academy of Agricultural Sciences | Yue H.,Guangdong Agriculture Industry Business Polytechnic College | Luo G.,Guangdong Academy of Agricultural Sciences | Yang Q.,Guangdong Academy of Agricultural Sciences | Kuang Z.,Guangdong Academy of Agricultural Sciences
Pakistan Journal of Zoology | Year: 2014

Ciboria carunculoides is one of the major fungal pathogens that attack mulberry fruits and cause huge lose of mulberry production. Many Trichoderma spp. produce a wide variety of toxic and antibiotic metabolites that are active against plant pathogens. This study evaluated the biological control activity of Trichoderma hamatum against C. carunculoides. The results showed that C. carunculoides was not detected on mulberry leaves through out the collection period, whereas C. carunculoides was detected in mulberry fruits. T. hamatum spore, fermentation broth and ethylacetate extracts caused high radial growth inhibition and germination inhibition of C. carunculoides, with the inhibition value up to 80% and 90%, respectively. The LC50 of T. hamatum ethylacetate extract in radial growth inhibition of C. carunculoides was 778.4 μg/ml. The use of T. hamatum offered a promising, safe and effective alternative to fungicides in treatment against popcorn disease of mulberry. © 2014 Zoological Society of Pakistan.


Yuan L.,Guangdong Agriculture Industry Business Polytechnic College | Liu B.,Guangdong Agriculture Industry Business Polytechnic College | Yin K.,Guangdong Agriculture Industry Business Polytechnic College | Yang G.,Institute of Microorganism Research of Guangzhou | And 2 more authors.
Analytical Letters | Year: 2013

The development of rapid, simple, and sensitive analytical methods for food contaminants is a topic of considerable interest. Polyclonal antibody against parathion-methyl (PM) was raised and used to develop a biotin-streptavidin indirect competitive enzyme-linked immunosorbent assay (BS-icELISA) with improved sensitivity for the determination of PM in vegetable. At the optimum conditions, the IC50 and limit of detection of BS-icELISA for PM were found to be 1.2 μg/L and 0.2 μg/L, respectively, which is 6-fold more sensitive than the traditional icELISA. This method was applied to determine PM residue in three vegetable samples with a simple and effective extraction procedure, good recoveries (in the range of 88.2-108.7% and with coefficients of variation below 15%) and accuracy (correlation coefficient of 0.9576 with GC-MS) were obtained. Our results indicated that the biotin-streptavidin system is useful in improving the sensitivity of ELISA and could be used for routine monitoring of pesticide at trace level. © 2013 Copyright Taylor and Francis Group, LLC.


Gao J.,South China University of Technology | Quan Y.,Guangdong Agriculture Industry Business Polytechnic College
International Journal of Applied Environmental Sciences | Year: 2013

Elliptical tubes with high efficiency and energy saving, reducing energy consumption, to protect environment, so getting a lot of attention.In the paper, study on the factors such as the ratio of the minor axis and the major axis, and tube distance, and tube diameter, and tube array of elliptic tube, etc. affect the heat transfer greatly, so optimizing these parameters is conducive to the application and design of elliptic tube and as theoretical guidance. In the paper, the new entropy resistance algorithm is put forward, and the physics model and mathematic model suitable to the problem are built, and the above mentioned parameters optimized. The test shows that the optimization maximizes the total heat transfer rate between the given volume and the given external fluid flow in a finite volume. The numerical results show that the heat transfer density is greater about 15% than that of the circle one when the Re of air in the range of 300 ≤ Re ≤ 900.Also verified, the entropy resistance mechanism considering fluid resistance factors, so the mechanism optimization of elliptic tube parameters more accurate, accord with the actual analysis of heat transfer mechanism of convection. © Research India Publications.

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