Guangdong Agriculture Industry Business Polytechnic College

Guangzhou, China

Guangdong Agriculture Industry Business Polytechnic College

Guangzhou, China
SEARCH FILTERS
Time filter
Source Type

Dong X.-X.,South China Agricultural University | Dong X.-X.,Nanjing Southeast University | Yuan L.-P.,Guangdong Agriculture Industry Business Polytechnic College | Liu Y.-X.,South China Agricultural University | And 5 more authors.
Talanta | Year: 2017

Progesterone (P4) is a kind of hormone that can cause neuropathic disturbances in humans when the concentration overpasses a certain degree. In this work, an electrochemical immunosensor capable of detecting P4 sensitively and selectively was developed. Thionine-graphene oxide (Thi-GO) composites with excellent biocompatibility were synthesized and coated to a clear glassy carbon electrode. P4 coating antigen (P4-OVA) was immobilized to the electrode, then sample as well as biotinylated antibody (biotin-P4 Ab) were added. The free P4 can compete with P4-OVA for binding to biotin-P4 Ab. After the further addition of streptavidin-HRP, H2O2 was introduced to develop electrical signal for quantitative determination of P4. After careful optimization of assay conditions, the proposed immunosensor showed a linear range from 0.02 to 20ngmL−1 for P4 in milk samples. The averaged recoveries from spiked samples ranged from 84.0% to 102.0%, which correlated well with standard HPLC-MS/MS. The biosensor also showed good specificity, reproducibility and stability, indicating its potential application in monitoring of P4 in a simple and low cost manner. © 2017 Elsevier B.V.


Chen X.,South China Agricultural University | Chen X.,Key Laboratory of Bio Pesticide Innovation and Application of Guangdong Province | Xie X.,Guangdong Agriculture Industry Business Polytechnic College | Ren S.,Key Laboratory of Bio Pesticide Innovation and Application of Guangdong Province | Wang X.,Key Laboratory of Bio Pesticide Innovation and Application of Guangdong Province
Biodiversity Data Journal | Year: 2016

Background Nephaspis indus Gordon, 1996 was imported into Taiwan from Hawaii in 1990 as a biological control agent for the spiralling whitefly, Aleurodicus dispersus Russell, 1965 (Hemiptera: Aleyrodidae). However, its establishment was not known prior to this study. New information Nephaspis indus Gordon, 1996, a natural enemy of Aleurodicus dispersus Russell (Hemiptera: Aleyrodidae) native to the Neotropical region, is recorded as established in Taiwan for the first time. The present paper provides a detailed further description and illustrations of the adult. Diagnostic characters for the genus and species are given and the nomenclature of this species is also discussed. © Chen X et al.


Liu W.,South China Agricultural University | Liu W.,Guangdong Agriculture Industry Business Polytechnic College | Ghouri F.,South China Agricultural University | Yu H.,South China Agricultural University | And 4 more authors.
PLoS ONE | Year: 2017

Common wild rice (Oryza rufipogon Griff.) is an important germplasm for rice breeding, which contains many resistance genes. Re-sequencing provides an unprecedented opportunity to explore the abundant useful genes at whole genome level. Here, we identified the nucleotide-binding site leucine-rich repeat (NBS-LRR) encoding genes by re-sequencing of two wild rice lines (i.e. Huaye 1 and Huaye 2) that were developed from common wild rice. We obtained 128 to 147 million reads with approximately 32.5-fold coverage depth, and uniquely covered more than 89.6% (> = 1 fold) of reference genomes. Two wild rice lines showed high SNP (single-nucleotide polymorphisms) variation rate in 12 chromosomes against the reference genomes of Nipponbare (japonica cultivar) and 93-11 (indica cultivar). InDels (insertion/deletion polymorphisms) count-length distribution exhibited normal distribution in the two lines, and most of the InDels were ranged from -5 to 5 bp. With reference to the Nipponbare genome sequence, we detected a total of 1,209,308 SNPs, 161,117 InDels and 4,192 SVs (structural variations) in Huaye 1, and 1,387,959 SNPs, 180,226 InDels and 5,305 SVs in Huaye 2. A total of 44.9% and 46.9% genes exhibited sequence variations in two wild rice lines compared to the Nipponbare and 93-11 reference genomes, respectively. Analysis of NBS-LRR mutant candidate genes showed that they were mainly distributed on chromosome 11, and NBS domain was more conserved than LRR domain in both wild rice lines. NBS genes depicted higher levels of genetic diversity in Huaye 1 than that found in Huaye 2. Furthermore, protein-protein interaction analysis showed that NBS genes mostly interacted with the cytochrome C protein (Os05g0420600, Os01g0885000 and BGIOSGA038922), while some NBS genes interacted with heat shock protein, DNA-binding activity, Phosphoinositide 3-kinase and a coiled coil region. We explored abundant NBSLRR encoding genes in two common wild rice lines through genome wide re-sequencing, which proved to be a useful tool to exploit elite NBS-LRR genes in wild rice. The data here provide a foundation for future work aimed at dissecting the genetic basis of disease resistance in rice, and the two wild rice lines will be useful germplasm for the molecular improvement of cultivated rice. © 2017 Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the originalauthor and source are credited.


PubMed | Biocontrol and Guangdong Agriculture Industry Business Polytechnic College
Type: | Journal: ZooKeys | Year: 2016

Five species of the genus


Xie X.-F.,South China Agricultural University | Xie X.-F.,Guangdong Agriculture Industry Business Polytechnic College | Yan H.-F.,CAS South China Botanical Garden | Wang F.-Y.,Shanghai Chenshan Botanical Garden | And 3 more authors.
Journal of Systematics and Evolution | Year: 2012

To investigate the mechanisms responsible for the high diversity in central and adjacent southwestern China, we inferred the phylogeographic history of Primula ovalifolia from chloroplast DNA data. One hundred and thirty five individuals from 13 natural populations (including one Primula tardiflora population) were analyzed. A total of 23 haplotypes were identified. Most of them were exclusive to a single population. Strong phylogeography structure was detected, with NST (0.936) significantly higher than GST (0.784). Phylogenetic analysis showed that all haplotypes were clustered into three lineages (clades I, II, and III). High genetic diversity was revealed, possibly due to the interglacial contraction and glacial expansion. The three identified lineages may have undergone different historical processes after the mid-Pleistocene, due to their early divergent time. Multiple refugia were inferred for the three lineages. Pleistocene climatic oscillations, combined with the complex local topography, were responsible for the strong phylogeographic pattern of P. ovalifolia. Our analysis supports the hypothesis that the central and adjacent southwestern region of China was an important refugium for the survival, persistence, and further speciation of most East Asia flora, which has led to high species diversity in this region. © 2012 Institute of Botany, Chinese Academy of Sciences.


Yan H.-F.,CAS South China Botanical Garden | Liu Y.-J.,South China Agricultural University | Xie X.-F.,Guangdong Agriculture Industry Business Polytechnic College | Zhang C.-Y.,South China Agricultural University | And 3 more authors.
PLoS ONE | Year: 2015

The genus Primula is extremely diverse in the east Himalaya-Hengduan Mountains (HHM) in China as a result of rapid radiation. In order to overcome the difficulty of morphological classification of this genus, we surveyed three plastid regions (rbcL, matK, and trnH-psbA) and two nuclear markers (ITS and ITS2) from 227 accessions representing 66 Primula species across 18 sections, to assess their discriminatory power as barcodes. We found that ITS alone or combined with plastid regions showed the best discrimination across different infrageneric ranks and at species level. We suggest rbcL + matK + ITS as the first choice at present to barcode Primula plants. Although the present barcoding combination performed poorly in many closely related species of Primula, it still provided many new insights into current Primula taxonomy, such as the underlying presence of cryptic species, and several potential improper taxonomic treatments. DNA barcoding is one useful technique in the integrative taxonomy of the genus Primula, but it still requires further efforts to improve its effectiveness in some taxonomically challenging groups. © 2015 Yan et al.


Tang H.,Guangdong Agriculture Industry Business Polytechnic College | Tang H.,CAS South China Botanical Garden | Deng Y.,CAS South China Botanical Garden
Phytotaxa | Year: 2016

Justicia latiflora Hemsl. in Forbes & Hemsley (1890: 245) is a species endemic to China occurring in Chongqing, Guangxi, Guizhou, Hubei, Hunan and Yunnan (Hu 2002, Hu et al. 2011). Later, it was transferred to Mananthes Bremekamp (1948: 59) as M. latiflora (Hemsl.) C.Y.Wu & C.C. Hu in Hu (2002: 295). Mananthes is one of the genera separated from Justicia Linnaeus (1753: 15) by Bremekamp to accommodate those Asian species having terminal spikes and it now is treated as a synonym of Justicia (Scotland & Vollesen 2000, Hu et al. 2011). This species differs from differ from those Chinese species formerly included in Mananthes in its bracts being purplish towards the apex and longer than the calyces, with corollas ca. 2 cm long. Three collections (Henry 3412, 3412A, 3412B) were cited from K in the protologue. Since no holotype was indicated they all are syntypes according to Art. 9.5 (ICN, McNeill et al. 2012). According to Art. 9.12, the lectotype must be chosen from those syntypes in first place. Comparing the three syntypes at K with the original description, we found that all are a match and candidates for serving as a lectotype. Since Henry 3412 (K000884035) is the only specimen with open corollas, a feature described in the protologue, it is here selected as the lectotype. © 2016 Magnolia Press.


PubMed | South China Agricultural University, Guangdong Agriculture Industry Business Polytechnic College and CAS South China Botanical Garden
Type: Journal Article | Journal: PloS one | Year: 2015

The genus Primula is extremely diverse in the east Himalaya-Hengduan Mountains (HHM) in China as a result of rapid radiation. In order to overcome the difficulty of morphological classification of this genus, we surveyed three plastid regions (rbcL, matK, and trnH-psbA) and two nuclear markers (ITS and ITS2) from 227 accessions representing 66 Primula species across 18 sections, to assess their discriminatory power as barcodes. We found that ITS alone or combined with plastid regions showed the best discrimination across different infrageneric ranks and at species level. We suggest rbcL + matK + ITS as the first choice at present to barcode Primula plants. Although the present barcoding combination performed poorly in many closely related species of Primula, it still provided many new insights into current Primula taxonomy, such as the underlying presence of cryptic species, and several potential improper taxonomic treatments. DNA barcoding is one useful technique in the integrative taxonomy of the genus Primula, but it still requires further efforts to improve its effectiveness in some taxonomically challenging groups.


Yuan L.,Guangdong Agriculture Industry Business Polytechnic College | Liu B.,Guangdong Agriculture Industry Business Polytechnic College | Yin K.,Guangdong Agriculture Industry Business Polytechnic College | Yang G.,Institute of Microorganism Research of Guangzhou | And 2 more authors.
Analytical Letters | Year: 2013

The development of rapid, simple, and sensitive analytical methods for food contaminants is a topic of considerable interest. Polyclonal antibody against parathion-methyl (PM) was raised and used to develop a biotin-streptavidin indirect competitive enzyme-linked immunosorbent assay (BS-icELISA) with improved sensitivity for the determination of PM in vegetable. At the optimum conditions, the IC50 and limit of detection of BS-icELISA for PM were found to be 1.2 μg/L and 0.2 μg/L, respectively, which is 6-fold more sensitive than the traditional icELISA. This method was applied to determine PM residue in three vegetable samples with a simple and effective extraction procedure, good recoveries (in the range of 88.2-108.7% and with coefficients of variation below 15%) and accuracy (correlation coefficient of 0.9576 with GC-MS) were obtained. Our results indicated that the biotin-streptavidin system is useful in improving the sensitivity of ELISA and could be used for routine monitoring of pesticide at trace level. © 2013 Copyright Taylor and Francis Group, LLC.


Gao J.,South China University of Technology | Quan Y.,Guangdong Agriculture Industry Business Polytechnic College
International Journal of Applied Environmental Sciences | Year: 2013

Elliptical tubes with high efficiency and energy saving, reducing energy consumption, to protect environment, so getting a lot of attention.In the paper, study on the factors such as the ratio of the minor axis and the major axis, and tube distance, and tube diameter, and tube array of elliptic tube, etc. affect the heat transfer greatly, so optimizing these parameters is conducive to the application and design of elliptic tube and as theoretical guidance. In the paper, the new entropy resistance algorithm is put forward, and the physics model and mathematic model suitable to the problem are built, and the above mentioned parameters optimized. The test shows that the optimization maximizes the total heat transfer rate between the given volume and the given external fluid flow in a finite volume. The numerical results show that the heat transfer density is greater about 15% than that of the circle one when the Re of air in the range of 300 ≤ Re ≤ 900.Also verified, the entropy resistance mechanism considering fluid resistance factors, so the mechanism optimization of elliptic tube parameters more accurate, accord with the actual analysis of heat transfer mechanism of convection. © Research India Publications.

Loading Guangdong Agriculture Industry Business Polytechnic College collaborators
Loading Guangdong Agriculture Industry Business Polytechnic College collaborators