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Hou T.,Guangdong Academy of Medicine Science | Huang D.,Guangzhou Hospital of Traditional Chinese Medicine
Molecular Medicine Reports

Disease biomarkers for diagnostic and prognostic purposes are most likely within an extremely low concentration range and are thus masked by the presence of high-abundance proteins. Therefore, removing high-abundance proteins is the main challenge for identifying disease biomarkers. In addition, the solution obtained from high-abundance protein depletion kits contains a rich array of compounds, which interfere with isoelectric focusing (IEF). In the present study, the effect of two commercial kits was evaluated and the downstream IEF protocol was optimized. High-resolution results could be obtained according to the following conditions: The ProteoPrep Blue Albumin and IgG Depletion kit depleted albumin and IgG; immobilized pH gradient strips (typically 18 cm) were rehydrated with sample buffer containing 250 μg serum proteins at 30 v for 6 h, 60 v for 6 h, 200 v for 2 h, 500 v for 2 h, 1,000 v for 2 h, 5,000 v for 2 h, 10,000 v for 2 h and then focusing at 10,000 v up to 110 k vhs. In addition, the protein spots identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry demonstrated that all proteins had a low abundance. The present study not only provides a definite and effective method for removing high-abundance proteins, but also provides a proper protocol (protocol C) for downstream IEF. The present study includes a comprehensive investigation of serum proteomics, which paves the way for serum protein research. Source

Wu H.-J.,Guangdong Academy of Medicine Science | Zeng H.,Guangdong Academy of Medicine Science | Liang C.-H.,Guangdong Academy of Medicine Science | Cui Y.-H.,Guangdong Academy of Medicine Science | And 2 more authors.
Chinese Journal of Medical Imaging Technology

Objective: To explore the imaging features of benign fibrous histiocytoma (BFH) of long bones. Methods: Imaging findings of 9 patients with BFH proved pathologically were retrospectively analyzed. X-ray plain film were obtained in all patients, and 4 patients underwent MR, 4 patients underwent CT scanning. Results: All lesions appeared as solitary. BFH occurred in the tibia (5 patients), femur (3 patients), fibula (1 patients). X-ray showed osteolytic lesions with eccentric thinning of the cortex. Sclerosis was found in the margin of the lesions to variable extent. CT revealed dense soft tissue in the lesions. Two lesions were restricted to bone, 2 tibia lesions destroyed the cortex but soft tissue mass did not form. Periosteal reaction was not found in alll lesions. MRI manifestations included a hypo to isointense lesion on T1WI and hyperintense signal intensity on T2WI. There was homogeneous or heterogeneous moderate to significant enhancement of the tumor after administration of contrast medium. Conclusion: Imaging features of BFH in long bones can provide clues for clinical diagnosis. Source

Liang Y.-H.,Guangdong Academy of Medicine Science | Liu L.,Guangdong Academy of Medicine Science | Chen D.-L.,Guangdong Academy of Medicine Science | Lin C.-Y.,Guangdong Academy of Medicine Science | And 3 more authors.
Journal of Huazhong University of Science and Technology - Medical Science

Lead placement for ventricular pacing variably impacts the physiological benefit of the patient. This study evaluated the ventricular lead performance and safety of right ventricular outflow tract septal pacing in patients with bradyarrhythmia in South China over 60-month follow-up. Totally, 192 patients (108 males, and 84 females, 63±21 years old) with bradyarrhythmia were randomly divided into two groups. The right ventricular outflow tract septum (RVOTs) group had lead placement near the septum (n=97), while the right ventricular apex (RVA) group had a traditional apical placement (n=95). RV septal lead positioning was achieved with a specialized stylet and confirmed using fluoroscopic projection. All patients were followed up for 60 months. Follow-up assessment included stimulation threshold, R-wave sensing, lead impedance and lead complications. The time of electrode implantation in both the ROVTs and RVA groups were significantly different (4.29±0.61 vs. 2.16±0.22 min; P=0.009). No differences were identified in threshold, impedance or R-wave sensing between the two groups at 1st, 12th, 36th and 60th month during the follow-up period. No occurrence of electrode displacement, increased pacing threshold or inadequate sensing was found. The long-term active fixation ventricular electrode performance in RVOTs group was similar to that in RVA group. RVOTs pacing near the septum using active fixation electrodes may provide stability during long-term follow-up period. © 2015, Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg. Source

Zhang Y.,Guangdong Academy of Medicine Science | Zheng Q.-J.,Guangdong Academy of Medicine Science | Wang S.,Guangdong Academy of Medicine Science | Zeng S.-X.,Guangdong Academy of Medicine Science | And 3 more authors.
American Journal of Infection Control

Background and Objective Observational studies have suggested an association between diabetes mellitus and the risk of surgical site infections (SSIs), but the results remain inconclusive. We conducted a meta-analysis of prospective cohort studies to elucidate the relationship between diabetes mellitus and SSIs. Methods We searched PubMed, Embase, and Web of Science databases and reviewed the reference lists of the retrieved articles to identify relevant studies. Associations were tested in subgroups representing different patient characteristics and study quality criteria. The random-effect model was used to calculate the overall relative risk (RR). Results Fourteen prospective cohort studies (N = 91,094 participants) were included in this meta-analysis, and the pooled crude RR was 2.02 (95% confidence interval, 1.68-2.43) with significant between-study heterogeneity observed (I2 = 56.50%). Significant association was also detected after we derived adjusted RRs for studies not reporting the adjusted RRs and calculated the combined adjusted RR of the 14 studies (RR, 1.69; 95% confidence interval, 1.33-2.13). Results were consistent and statistically significant in all subgroups. Stratified analyses found the number of confounders adjusted for, sample size, and method of diabetes case ascertainment might be the potential sources of heterogeneity. Sensitivity analysis further demonstrated the robustness of the result. Conclusions This meta-analysis suggests diabetes mellitus is significantly associated with increased risk of SSIs. Future studies are encouraged to reveal the mechanisms underlying this association. © 2015 Association for Professionals in Infection Control and Epidemiology, Inc. Source

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