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Khargon, India

Ajay R.,Siksha O' Anusandhan University | Kar D.M.,Siksha O' Anusandhan University | Makkar Renu R.,GRY Institute of Pharmacy
International Journal of Drug Development and Research | Year: 2013

Natural products are used as traditional medicines from ancient times. From ancient times human beings depended on plants for their food and health purpose. A wide range of plants posses medicinal properties, different parts of plants such as root, stem, flower, fruit, or whole plants are used to cure various health calamities. But the infectious diseases caused by microbes remains a major confront for science even today. It is necessary to have ideal anti-microbial products from plant products because they are considered safer and economic. One such medicinal plant species is Ipomoea reniformis, which belongs to convolvulaceae family and Lamiidae subclass. Its antimicrobial activity was evaluated with aqueous, benzene, ethyl acetate, chloroform and ethanolic extract. The investigation was carried out against various gram positive and gram negative bacterial strains (Escherichia coli NCIM 2109; Staphylococcus aureus NCIM 2079, Pseudomonas aeruginosa NCIM 2036; Bacillus subtilis NCIM 2250). Antifungal activity was carried on (Aspergillus niger NCIM 545). Well diffusion method was employed for the detection of antimicrobial activity. Streptomycin and Amphoterecin B were used as standard. The aqueous extract exhibited activity against Staphylococcus aureus, while the chloroform & ethanolic extract elucidated antifungal activity against Aspergillus niger. © 2013 By IYPF All rights reserved.

Rokade Y.,Psgvpms Institute Of Pharmacy | Dongare N.,GRY Institute of Pharmacy
Rasayan Journal of Chemistry | Year: 2010

Azetidinone derivatives were synthesized from β-naphthol in two steps. First the Schiff's bases were prepared by reacting the hydrazine of a naphthalene derivative with different aromatic aldehydes.Cyclocondensation of the Schiff's bases with chloroacetyl chloride in the presence of triethylamine resulted in the formation of corresponding azetidinone analogues. The structures of the newly synthesized compounds were confirmed by IR, 1H NMR, and Mass spectroscopic analysis The in vitro antibacterial and antifungal activity of compound have been evaluated by paper disc diffusion method. © 2010 RASĀYAN. All rights reserved.

Rao T.N.,Krishna University | Sreenivasulu D.,Andhra University | Sreenivasula Reddy E.G.,Andhra University | Devi K.S.,GRY Institute of Pharmacy
Research Journal of Pharmacy and Technology | Year: 2014

A simple, sensitive and inexpensive method was developed using matrix solid-phase dispersion (MSPD), together with high performance liquid chromatographic method for determination of Tetracycline residues and its metabolite 4- Epitetracycline in cow milk. The evaluated parameters included the type and amount of sorbent (silica gel, C18 and alumina) and the nature of eluent (20% trichloro acetic acid, 0.01M citric acid, 0.01M disodium hydrogen phosphate, 0.01M EDTA and Methanol). The best results were obtained using 10 mL of milk sample, 1.0 g of C18 as sorbent and 20mL of 20% trichloroacetic acid, 0.01M citric acid, 0.01M disodium hydrogen phosphate, 0.01M EDTA and Methanol (1:2:2:2:3, (v/v)).The method was validated using in milk samples spiked with Tetracycline residues and its metabolite 4-Epitetracycline at different concentration levels (0.05 and 0.5 μg/mL). Average recoveries (using each concentration six replicates) ranged 87-96%, with relative standard deviations less than 2%, calibration solutions concentration in the range 0.05-5.0 μg/mL and limit of detection (LOD) and limit of quantification (LOQ) were 0.02 μg/mL and 0.05 μg/mL respectively. © RJPT All right reserved.

Karpillai M.,GRY Institute of Pharmacy | Dhangar S.,GRY Institute of Pharmacy
Research Journal of Pharmacy and Technology | Year: 2011

A reversed phase HPLC method is developed for the determination of glipizide in pharmaceutical dosage form. Chromatography was carried out on an inertsil C18 column using a mixture of methanol and phosphate buffer (pH 3.4) (55:45 v/v) as the mobile phase at a flow rate of 1ml/min. Detection was carried out at 225nm. The retention time of the drug was 7.098min. The method produced linear responses in the concentration range of 15-75 μg/ml of Glipizide. The method was found to be applicable for the determination of the drug in tablets. © RJPT All right reserved.

Namdev N.,GRY Institute of Pharmacy | Upadhyay S.,GRY Institute of Pharmacy
Research Journal of Pharmacy and Technology | Year: 2016

Recent advances in biotechnology allow the selection and the preparation of novel macromolecular compounds such as peptides, proteins and DNA analogs (produced by Hybridoma cell technology and Recombinant DNA technology) to be used as drugs (e.g., hormones, monoclonal antibodies, vaccines) for therapeutic purposes. Such compounds show powerful and selective therapeutic activity, but unfortunately they must often be dropped at some development stage, because of their high enzymatic susceptibility, short shelf life or unsuitable efficacy after the administration to the patient, owing to immunogenic reactions or poor bioavailability. A number of approaches have been used to overcome these limitations. As these therapeutic proteins and peptides are made available, it will be essential to formulate these drugs into safe and effective delivery systems. Due to its wider applications in pharmaceutical industries, they will replace many existing organic based pharmaceuticals. Now days, many drugs are in the world market, while several hundred are in clinical trials. This article reviews the various problems associated and novel approaches for formulation and development of oral protein and peptide drug delivery systems. © RJPT All right reserved.

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