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Garcia Y.H.,Grupo Empresarial LABIOFAM | Salomon Izquierdo S.,Research Center sarrollo Of Medicamentos Cidem | Acosta Esquijarrosa J.,Instituto Superior Polytechnic Jose A Echevarria | Romero Diaz A.,Research Center sarrollo Of Medicamentos Cidem | And 2 more authors.
Revista Cubana de Plantas Medicinales | Year: 2016

Introduction: Annona muricata L. (soursop) is used in ethnomedicine for the treatment of various diseases. Leaves of this species have been found to contain flavonoids, which are compounds with antidiabetic, antioxidant and anti-inflammatory properties. Objective: evaluate the influence of operational parameters on the extraction of flavonoids from A. muricata leaves. Methods: dry ground leaves were used as plant material and ethanol as solvent. Evaluation of the operational variables was based on a 23 experimental rotatable response surface central composite design with star points, selecting the intervals between 50 and 70 ml/g for the plant material/solvent volume ratio, 40% to 80% for ethanol concentration and 2 to 3 h for extraction time. Flavonoids and quercetin were found in the extracts obtained. Total flavonoids were quantified using a colorimetric method at 430 nm, expressed as quercetin. Experiments were conducted in triplicate to determine the best experimental condition. Comparison with the design value predicted showed a good correlation between the two. Results: the optimal condition for extraction of total flavonoids was reached at an ethanol concentration of 96%, a 70 ml/g ratio and a time of 1.6 h. The variables found to be significant were the quadratic effects of the plant material/solvent volume ratio and ethanol concentration. Conclusions: a selection was made of the best conditions to obtain an extract from soursop leaves containing flavonoids, in which an extraction output of 87% was achieved in the extraction process. © 2016, Editorial Ciencias Medicas. All rights reserved.


Diaz-Miranda M.,Grupo Empresarial LABIOFAM | Campos T.,Grupo Empresarial LABIOFAM | Perez J.E.,Grupo Empresarial LABIOFAM | Paneque D.,Grupo Empresarial LABIOFAM
Biotecnologia Aplicada | Year: 2012

Equine Infectious Anemia Virus (EIAV) is the causal agent of equine infectious anemia. Plasma virus RNA levels and the fraction of infected cells are both very low during the chronic stage of EIAV infections, turning the amplification of viral genes into a technically demanding task. In this work, DNA was extracted from the spleen of a chronically infected animal previously immunosuppressed with prednisolone, amplifying by nested PCR and sequencing the segment of the gag gene that codes for the p26 protein. Phylogenetic analysis placed the Cuban strain closest to three Canadian strains. The translated protein sequence exhibits 7 differences with the most related Canadian strain (Can7) and 13 with the Wyoming strain. This is the first report of a partial nucleotide sequence from EIAV in Cuba and the Caribbean.


Dominguez A.,Grupo Empresarial LABIOFAM | Polanco R.,Grupo Empresarial LABIOFAM | Cossio G.,Institute Informacion Cientifica y Tecnologica | Morejon Y.,Institute Informacion Cientifica y Tecnologica | Riquenes Y.,Grupo Empresarial LABIOFAM
Biotecnologia Aplicada | Year: 2014

The dynamism observed in the area of veterinary vaccinology needs to be monitored by the biopharmaceutical industry, thus, to anticipate the possible changes and take actions that allow it to adapt to the future scenario. On this basis, a study was performed to assess the current status, trends, scientific and technological projections in the production of veterinary vaccines. To this end, a topic search was done, on vaccines for poultry, cattle, pigs, rabbits, horses, fsh and dogs, in the Scopus database from 2008 to 2012 2008-2012 was done and further contextualized to early 2014. It was found that veterinary vaccinology is a booming field, with the highest growth in the year 2011 with 18.05 % compared to 2008. As such, the US and China are leading this issue, having a major scientific interest in protein antigens, adjuvants and viral vaccines produced with conventional technologies. The most attended species are birds, pigs and cattle, the frst species with 15.21 % of all publications. We conclude that the dominance of the classical vaccine production technologies will continue despite the advances in genetic engineering and biotechnology. Vaccines produced with modern technologies seem to be, at least for now, less affordable for the producer, and therefore, impractical. Similarly, the next few years will be very important for the development and registration of new vaccines obtained by DIVA technology and their diagnostic systems.


Dominguez A.,Grupo Empresarial Labiofam | Polanco R.,Grupo Empresarial Labiofam | Cossio G.,Institute Informacion Cientifica y Tecnologica | Morejon Y.,Institute Informacion Cientifica y Tecnologica | Riquenes Y.,Grupo Empresarial Labiofam
Biotecnologia Aplicada | Year: 2014

The dynamism observed in the area of veterinary vaccinology needs to be monitored by the biopharmaceutical industry, thus, to anticipate to the possible changes and take actions that allow it to adapt to the future scenario. On this basis, a study was performed to assess the current status, trends, scientific and technological projections in the production of veterinary vaccines. To this end, a topic search was done on vaccines for poultry, cattle, pigs, rabbits, horses, fish and dogs, in the Scopus database from 2008 to 2012 and further contextualized to early 2014. It was found that veterinary vaccinology is a booming field, with the highest growth in the year 2011 with 18.05 % compared to 2008. As such, the US and China are leading this issue, having a major scientific interest in protein antigens, adjuvants and viral vaccines produced with conventional technologies. The most attended species are birds, pigs and cattle, the first species with 15.21 % of all publications. We conclude that the dominance of the classical vaccine production technologies will continue despite the advances in genetic engineering and biotechnology. Vaccines produced with modern technologies seem to be, at least for now, less affordable for the producer, and therefore impractical. Similarly, the next few years will be very important for the development and registration of new vaccines obtained by DIVA technology and their diagnostic systems.


Classical swine fever (CSF) is a highly contagious viral disease caused by a RNA virus that belongs to the genus Pestivirus within the family Flaviviridae. CSF represents one of the leading worldwide threats to the pig industry. Life attenuated vaccines using lapinized Chinese strain have been used to prevent the disease. The reverse transcriptase polymerase chain reaction (RT-PCR) is one of the more sensitive methods that have been used in veterinary medicine for RNA virus detection. In the case of CSF virus is very useful, because the nucleic acid could be detected in early stages of the infection and during a long period in recovered animals. The aim of this study was to apply real time RTPCR assay for the detection of lapinized Chinese strain from the Cuban vaccine strain against CSF. Tonsils of vaccinated pigs were the most positive organ in the detection of RNA vaccinal virus. Interference of the vaccinal virus in diagnosis was proved. The lower threshold cycle values were seen at 12 postvaccination day. © 2016, Finlay Ediciones. All rights reserved.

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