Doñinos de Salamanca, Spain
Doñinos de Salamanca, Spain

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Alcalde-Eon C.,Grupo de Investigacion en Polifenoles GIP | Rivas-Gonzalo J.C.,Grupo de Investigacion en Polifenoles GIP | Munoz O.,University of Chile | Escribano-Bailon M.T.,Grupo de Investigacion en Polifenoles GIP
Phytochemistry | Year: 2013

Three different pollination syndromes (bee, hummingbird and moth-pollination syndromes) have been described for the different species of the genus Schizanthus Ruiz & Pav. (Solanaceae). Two closely related species from a phylogenetic point of view, Schizanthus grahamii and Schizanthus hookeri, show hummingbird and bee-pollination syndromes, respectively. One of the traits used to assign these pollination syndromes is the colour of the petals, which is red in S. grahamii and bluish-pink in S. hookeri. The objective of this work was to establish the differences in the anthocyanin composition of these two Schizanthus species that contribute to the different pollination syndrome. Identification of the pigments was carried out from the chromatographic and spectral data supplied by the HPLC-DAD-MSn analyses of the samples. Alkaline and acid hydrolyses were also performed in the extracts and in some isolated compounds in order to confirm the identities. First difference between these two species was found in the total anthocyanin content, which was about 13-fold higher in S. grahamii than in S. hookeri. Furthermore, the major peak was also different in both cases, thus explaining quantitative and qualitative colour differences between species. Delphinidin 3-O-rutinoside represented ca. 72% of the total area in S. grahamii and petanin (Petunidin 3-O-p- coumaroylrutinoside-5-O-glucoside) accounted for almost 62% of the total area in S. hookeri. The presence of the p-coumaroylrutinosyl moiety in the petanin makes the intramolecular copigmentation possible, thus conferring the bluish-pink hue to the petals of S. hookeri. Delphinidin 3-O-rutinoside is in turn responsible for the red colour exhibited by the petals of S. grahamii and along with other floral traits, responsible for the attraction of hummingbirds to the plant. Pigments structurally related to petanin, which have been often detected in other genus of Solanaceae, were detected in both species and in similar contents, supporting results from previous studies that pointed to a bee-pollinated common ancestor for both species that further specialised acquiring traits attractive to hummingbirds and among them, red colour of the petals which is mainly supplied by delphinidin 3-O-rutinoside. © 2012 Elsevier Ltd. All rights reserved.


Garcia-Estevez I.,Grupo de Investigacion en Polifenoles GIP | Escribano-Bailon M.T.,Grupo de Investigacion en Polifenoles GIP | Rivas-Gonzalo J.C.,Grupo de Investigacion en Polifenoles GIP | Alcalde-Eon C.,Grupo de Investigacion en Polifenoles GIP
Analytica Chimica Acta | Year: 2010

During maturation and ageing in oak barrels wines improve their organoleptic properties. Ellagitannins can be released from wood to the wine and be involved in oxidation reactions and seem to influence the astringency and colour properties of the wine. Nevertheless, the ellagitannins levels are lower than those of other wine constituents and, consequently, they are not easily detected. This study has developed a two-step fractionation method consisting of a solid phase extraction in C-18 Sep-Pak® cartridges followed by size exclusion chromatography in hand-packed Sephadex LH-20 minicolumn for the detection of oak ellagitannins in different types of wines. An HPLC method has also been developed which allows the separation of compounds with the same m/z ratios, facilitating the ellagitannin identification by means of the mass spectrometric analyses. The main oak ellagitannins (grandinin, vescalagin, roburin E and castalagin) were isolated, detected separately and identified in a spiked wine and in three real ones, proving the usefulness of the fractionation method. © 2009 Elsevier B.V. All rights reserved.


Alcalde-Eon C.,Grupo de Investigacion en Polifenoles GIP | Garcia-Estevez I.,Grupo de Investigacion en Polifenoles GIP | Martin-Baz A.,Bodegas Vinas del Cambrico S.A. | Rivas-Gonzalo J.C.,Grupo de Investigacion en Polifenoles GIP | Escribano-Bailon M.T.,Grupo de Investigacion en Polifenoles GIP
Biochemical Systematics and Ecology | Year: 2014

First report on anthocyanin and flavonol profiles of Rufete (Vitis vinifera L.) red grapes. Rufete and Tempranillo grapes showed different anthocyanin and flavonol profiles. Application of hierarchical cluster analyses allows differentiation between Rufete and Tempranillo. Unlike flavonol profile, anthocyanin profile allows further differentiation among Rufete clones. © 2014 Elsevier Ltd.

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