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Reis E.C.,State University of Rio de Janeiro | Aires R.M.,State University of Rio de Janeiro | Moura J.F.,Grupo de Estudos de Mamiferos Marinhos da Regiao Dos Lagos | Matias C.A.R.,Grupo de Estudos de Mamiferos Marinhos da Regiao Dos Lagos | And 5 more authors.
Genetics and Molecular Research | Year: 2011

There have been few studies on Magellanic penguins (Spheniscus magellanicus). In 2008, these penguins washed ashore along the Brazilian coast in unusually high numbers, some reaching as far as northeast Brazil. As Magellanic penguins show little sexual dimorphism, sex determination by morphological features is not accurate. Here, we tested a molecular procedure for sexing specimens of S. magellanicus washed ashore along the coasts of Sergipe, Rio de Janeiro and Rio Grande do Sul in 2008, comparing the sex ratio between these localities. Tissue samples were collected from 135 dead, beached specimens. We carried out total genomic DNA extraction and CHD-Z/CHD-W gene amplification by PCR using P2 and P8 primers. Amplicons were separated by 12% acrylamide gel electrophoresis. We found a greater proportion of females (70%). Sex could be determined because females have two intronic regions of CHD gene of different size in the sex chromosomes, visualized as two bands on the gel (380 and 400 bp approximately), while males have only one (400 bp). Therefore, this method proved to be effective and sensitive for sex determination of S. magellanicus individuals. Data on sex ratios are useful for understanding the dynamics and ecology of Magellanic penguin populations. © FUNPEC-RP. Source


De Moura J.F.,PPG em Saude Publica e Meio Ambiente | Emin-Lima R.,PPG em Saude Publica e Meio Ambiente | Hacon S.S.,Grupo de Estudos de Mamiferos Marinhos da Regiao Dos Lagos | Vega C.M.,Pontifical Catholic University of Rio de Janeiro | And 2 more authors.
Bulletin of Environmental Contamination and Toxicology | Year: 2012

Total mercury (Hg) was analyzed in muscle tissue of 27 accidentally captured Guiana dolphins (Sotalia guianensis) in order to evaluate Hg contamination levels present in the Amazon Continental Shelf, in Amapá state, North Brazil. The samples showed a mean concentration of 0.4 ± 0.16 μg/g wet weight (ww), ranging from 0.07 to 0.79 μg/g ww. As observed in several other cetacean species, Hg concentrations presented positive correlations to body length, related to the capacity to bioaccumulate this element throughout life. Hg concentrations were not significantly different between males (mean = 0.38 μg/g ww; n = 15) and females (mean = 0.42 μg/g ww; n = 12). Concentrations were low when compared to results of studies carried out with small cetaceans in the Northern Hemisphere, and with some previous studies in the southeastern region of Brazil. In contrast with high Hg concentrations normally detected in river dolphin samples from Amazon River tributaries, our results suggest that the Amazon coast contains low levels of Hg in bioavailable form. © Springer Science+Business Media, LLC 2012. Source


De Moura J.F.,PPG em Saude Publica e Meio Ambiente | Hacon S.D.S.,Escola Nacional de Saude Publica ENSP FIOCRUZ | Vega C.M.,Pontifical Catholic University of Rio de Janeiro | Hauser-Davis R.A.,Pontifical Catholic University of Rio de Janeiro | And 2 more authors.
Bulletin of Environmental Contamination and Toxicology | Year: 2012

Total mercury (Hg) was determined in muscle tissue of 20 Guiana dolphins stranded along the coast of Rio de Janeiro state, Brazil, with a mean of 1.07 μg/g wet weight. Mercury concentrations were positively related to body length, possibly related to the capacity of the dolphins to bioaccumulate this element throughout life. The Hg concentrations were not significantly different between males and females, although females (1.08 μg/g) showed slightly higher levels than males (1.04 μg/g). Concentrations were low when compared to results of studies carried out with small cetaceans in the Northern Hemisphere, and with previous studies in the Southeastern Brazil. © 2011 Springer Science+Business Media, LLC. Source

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