Xu J.-T.,Growing Base for State Key Laboratory of New Fiber Materials and Modern Textile |
Xu J.-T.,Qingdao University |
Zhu P.,Growing Base for State Key Laboratory of New Fiber Materials and Modern Textile |
Zhu P.,Qingdao University |
And 8 more authors.
Wool Textile Journal | Year: 2015
A new method of alkali-reduction with two-bath in two-step was used to extract keratin from human hair efficiently. The optimum condition was concluded, a mass of human hair was processed with 0. 1 mol/L NaOH for 1 h. then was mixed with 0. 75 mol/L NaSO3, 8 mol/L urea, 0. 02 mol/L SDS according to a ratio of 1:15, and was deoxygenized at 80 °C for 5 h, its dissolution rate was around 55%. The keratin was analysed by SDS-PAGE and FTIR. The result revealed that the mass of the keratin is centralized between 25 kDa and 37 kDa. The extracted keratin was mainly composed by random-coil and conserved α-helix, β-Turn, β-sheet. Source