Cmr Group Of Institutions

Hyderabad, India

Cmr Group Of Institutions

Hyderabad, India
SEARCH FILTERS
Time filter
Source Type

Garige B.,Anurag Group of Institutions | Keshetti S.,Satavahana University | Vattikuti U.,Cmr Group Of Institutions
Pharmacognosy Research | Year: 2016

Background: Galphimia glauca is an evergreen shrub found across peninsular India, belonging to family Malpighiaceae. Objective: The objective of this study was to assess the in vivo depressant effects and muscle coordination activity of G. glauca stem methanol extract (GGSME). Materials and Methods: The stem methanol extract was administered in Swiss albino mice in 1 day to study the central nervous system (CNS) depressant and muscle coordination activity employing animal models such as sodium pentobarbital-induced sleep test, hole-board test, open field test, pentylenetetrazole (PTZ)-induced convulsions, picrotoxin-induced convulsions, grip strengthening test in mice, and Rota-rod test. Results: The LD50of GGSME was found to be >2000 mg/kg body weight (b.w.). Mice treated with stem methanol extract at 100, 200, and 400 mg/kg, b.w. doses extended the sleeping time induced by sodium pentobarbital (40 mg/kg. b.w., i.p.). The stem methanol extract at 400 mg/kg dose showed a significant (P ≤ 0.001) dose-dependent decrease in the number of rears and head dipping number in the hole-board test. The extract exhibited a significant (P ≤ 0.001) effect on the ambulatory behavior of mice in the open field test and also extended the onset of seizures induced by PTZ (90 mg/kg b.w., i.p.) and picrotoxin (10 mg/kg, b.w., i.p.). The extract also exhibited significant (P ≤ 0.001) effects on muscle coordination in rota-rod and grip strengthening test in mice. Conclusion: The study results conclude that the GGSME has a potential CNS depressant and muscle relaxant effects compared to the standard drugs. © 2016 Pharmacognosy Research | Published by Wolters Kluwer - Medknow.


Garige B.S.R.,Anurag Group of Institutions | Keshetti S.,Satavahana University | Vattikuti U.M.R.,Cmr Group Of Institutions
International Journal of PharmTech Research | Year: 2016

Objective: This study assesses the depressant effects and muscle relaxant activity of G. glauca leaf methanol extract. Methods: The leaf extract was administered in Swiss albino mice for one day to study depressant and muscle relaxant activity employing models like Sodium pentobarbital induced sleep test, Hole-board test, Open field test, Pentylenetetrazole induced convulsions, Picrotoxin induced convulsions, Grip strengthening test and Rota rod test. Results: The LD50 of the leaf extract was found to be > 2000 mg/kg b.w. Mice treated with leaf methanol extract at 100, 200 and 400 mg/kg, b.w doses prolonged the sleeping time induced by sodium pentobarbital (40 mg/kg. b.w, i.p.). The leaf methanol extract at 400 mg/kg dose showed a significant (P ≤ 0.001) sedative effect in the hole-board test and decreased spontaneous activity in mice and also delayed the onset of seizures induced by Pentylenetetrazole (90 mg/kg b.w, i.p) and Picrotoxin (10 mg/kg, b.w, i.p).The leaf extract showed significant (P ≤ 0.001) effect on the ambulatory behaviour of mice in open field test. The leaf methanol extract also showed significant (P ≤ 0.05) effects on muscle coordination in Grip strengthening and Rota rod test in mice (P ≤ 0.01). Conclusion: The results conclude that the Galphimia glauca leaf methanol extract has significant depressant and muscle relaxant effects compared to standard drugs. © 2016, Sphinx Knowledge House. All rights reserved.


Garige B.S.R.,Anurag Group of Institutions | Srisailam K.,Anurag Group of Institutions | Srisailam K.,Satavahana University | Vattikuti U.M.R.,Cmr Group Of Institutions
Asian Journal of Chemistry | Year: 2016

This study assesses the central nervous system depressant effects and muscle relaxant activity of methanolic extract of Galphimia gracilis leaf in Swiss albino mice employing models like open field test, hole-board test, sodium pentobarbital-induced sleep test, pentylenetetrazole induced convulsions, picrotoxin-induced convulsions, rota rod test and grip strengthening test. The LD50 results of the extract was found to be > 2000 mg/kg. The extract exhibited significant (P = 0.001) effects in both the open field test and hole-board test. The extract at 100, 200 and 400 mg/kg doses extended the duration of sleep induced by sodium pentobarbital (40 mg/kg) and also delayed the onset of seizures induced by pentylenetetrazole (90 mg/kg) and picrotoxin (10 mg/kg) respectively. The extract also exhibited significant (P = .001) effects in Grip strengthening test and Rota rod test. The study concludes that the methanolic extract of Galphimia gracilis leaf has significant depressant activity and muscle relaxant effects.


Rao Garige B.S.,Anurag Group of Institutions | Keshetti S.,Satavahana University | Rao Vattikuti U.M.,Cmr Group Of Institutions
Asian Journal of Chemistry | Year: 2016

This study assesses the analgesic and anti-inflammatory properties of methanolic extract of Galphimia glauca leaf using in vivo models. The dose range of 100, 200 and 400 mg/kg b.w was administered orally for assessing analgesic and anti-inflammatory activity respectively. Further studies were conducted for determining the involvement of central and peripheral receptor actions in the analgesic activity of the extract by pre-challenging it with naloxone and acetic acid respectively. The LD50 of the extract was found to be > 2000 mg/kg b.w. The leaf extract at 400 mg/kg dose exhibited significant (P ≤ 0.001) and dose-dependent analgesic activity. It also exhibited central and peripheral analgesic actions when treated with naloxone and acetic acid respectively. However, the extract of leaf has shown anti-inflammatory activity, which is not comparable to that of standard drug. The results revealed that the methanolic extract of Galphimia glauca leaf has potential in terms of analgesic properties. © 2016, Chemical Publishing Co. All rights reserved.

Loading Cmr Group Of Institutions collaborators
Loading Cmr Group Of Institutions collaborators