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Pontevedra, Spain

Novio S.,University of Santiago de Compostela | Cartea M.E.,Group of Genetics | Soengas P.,Group of Genetics | Freire-Garabal M.,University of Santiago de Compostela | Nunez-Iglesias M.J.,University of Santiago de Compostela
Molecules | Year: 2016

Despite the major progress made in the field of cancer biology, cancer is still one of the leading causes of mortality, and prostate cancer (PCa) is one of the most encountered malignancies among men. The effective management of this disease requires developing better anticancer agents with greater efficacy and fewer side effects. Nature is a large source for the development of chemotherapeutic agents, with more than 50% of current anticancer drugs being of natural origin. Isothiocyanates (ITCs) are degradation products from glucosinolates that are present in members of the family Brassicaceae. Although they are known for a variety of therapeutic effects, including antioxidant, immunostimulatory, anti-inflammatory, antiviral and antibacterial properties, nowadays, cell line and animal studies have additionally indicated the chemopreventive action without causing toxic side effects of ITCs. In this way, they can induce cell cycle arrest, activate apoptosis pathways, increase the sensitivity of resistant PCa to available chemodrugs, modulate epigenetic changes and downregulate activated signaling pathways, resulting in the inhibition of cell proliferation, progression and invasion-metastasis. The present review summarizes the chemopreventive role of ITCs with a particular emphasis on specific molecular targets and epigenetic alterations in in vitro and in vivo cancer animal models. © 2016 by the authors; licensee MDPI.

Branca F.,University of Catania | Ragusa L.,University of Catania | Tribulato A.,University of Catania | Velasco P.,Group of Genetics | Cartea M.E.,Group of Genetics
Acta Horticulturae | Year: 2013

Mediterranean basin represents an important centre of origin and diversification of Brassicaceae and above all of B. oleracea because are widespread several Brassica wild species (n=9) which seem support an intense genetic flux among them. In fact, the genetic self-incompatibility system characterize some wild populations of Brassica (n=9) and B. oleracea landraces and favours the genetic recombination and the set-up of several forms and types within the same crop, such as in broccoli, cauliflower, kale, etc. That is observed mainly for bio-morphological traits but we started to study their antioxidant profile. In this frame DISPA of Catania University and MBG (CSIC) of Pontevedra, Spain collected Brassica species (n=9) in view to define their glucosinolate profile. Cluster and principal component analysis were performed to determine relationships among accessions and to obtain information on this material. The dendrogram showed seven groups, one group including most of the populations and six small independent groups.

Lema M.,Group of Genetics | Lema M.,University of Santiago de Compostela | Cartea M.E.,Group of Genetics | Francisco M.,Group of Genetics | And 2 more authors.
Plant Breeding | Year: 2015

Productivity and quality of crops of Brassica rapa L. in north-western of Spain are highly affected by black rot, caused by the bacterium Xanthomonas campestris pv. campestris (Pammel) Dowson (Xcc). Several races of Xcc have been described in this area, being the race 6 the most frequent in B. rapa crops and races 1 and 4 the most frequent in B. oleracea crops. The control of the disease can be aided by the employment of resistant varieties. The aim of this work was to find sources of resistance to Xcc in a collection of open-pollinated varieties of B. rapa from north-western Spain. Resistance was evaluated in 191 landraces. Partial resistance to races 6, 1 and 4 and complete resistance to race 4 were identified in several landraces. Several accessions exhibited partial resistance to the three races. Sources of resistance were identified in landraces of different crops of the species (turnips, turnip greens and turnip tops). These landraces could be grown after selection for resistance or they can be donors of resistance genes in breeding programmes. © 2015 Blackwell Verlag GmbH.

Sotelo T.,Group of Genetics | Lema M.,University of Santiago de Compostela | Soengas P.,Group of Genetics | Cartea M.E.,Group of Genetics | Velasco P.,Group of Genetics
Applied and Environmental Microbiology | Year: 2015

Glucosinolates (GSLs) are secondary metabolites found in Brassica vegetables that confer on them resistance against pests and diseases. Both GSLs and glucosinolate hydrolysis products (GHPs) have shown positive effects in reducing soil pathogens. Information about their in vitro biocide effects is scarce, but previous studies have shown sinigrin GSLs and their associated allyl isothiocyanate (AITC) to be soil biocides. The objective of this work was to evaluate the biocide effects of 17 GSLs and GHPs and of leaf methanolic extracts of different GSL-enriched Brassica crops on suppressing in vitro growth of two bacterial (Xanthomonas campestris pv. campestris and Pseudomonas syringae pv. maculicola) and two fungal (Alternaria brassicae and Sclerotinia scletoriorum) Brassica pathogens. GSLs, GHPs, and methanolic leaf extracts inhibited the development of the pathogens tested compared to the control, and the effect was dose dependent. Furthermore, the biocide effects of the different compounds studied were dependent on the species and race of the pathogen. These results indicate that GSLs and their GHPs, as well as extracts of different Brassica species, have potential to inhibit pathogen growth and offer new opportunities to study the use of Brassica crops in biofumigation for the control of multiple diseases. © 2015, American Society for Microbiology.

Francisco M.,Group of Genetics | Ali M.,Group of Genetics | Ali M.,Ain Shams University | Ferreres F.,CSIC - Center of Edafology and Applied Biology of the Segura | And 3 more authors.
Frontiers in Plant Science | Year: 2016

Phenolic compounds are proving to be increasingly important for human health and in crop development, defense and adaptation. In spite of the economical importance of Brassica crops in agriculture, the mechanisms involved in the biosynthesis of phenolic compounds presents in these species remain unknown. The genetic and metabolic basis of phenolics accumulation was dissected through analysis of total phenolics concentration and its individual components in leaves, flower buds, and seeds of a double haploid (DH) mapping population of Brassica oleracea. The quantitative trait loci (QTL) that had an effect on phenolics concentration in each organ were integrated, resulting in 33 consensus QTLs controlling phenolics traits. Most of the studied compounds had organ-specific genomic regulation. Moreover, this information allowed us to propose candidate genes and to predict the function of genes underlying the QTL. A number of previously unknown potential regulatory regions involved in phenylpropanoid metabolism were identified and this study illustrates how plant ontogeny can affect a biochemical pathway. © 2016 Francisco, Ali, Ferreres, Moreno, Velasco and Soengas.

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