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Puerto Serrano, Spain

Matatagui D.,GRIDSEN | Fontecha J.,GRIDSEN | Fernandez M.J.,GRIDSEN | Oliver M.J.,University of Castilla - La Mancha | And 6 more authors.
Procedia Engineering | Year: 2012

The goal has been to develop a Love-wave device and a PDMS microfluidic chip in order to measure immunoreactions in liquid media, operating in dynamic mode (continuous flow-through). The obtained results have been compared with the most used acoustic wave device, the quartz crystal microbalance (QCM), which worked in static mode. It has been demonstrated once again that QCM is an excellent tool as immunosensor, although Lovewave device is a good alternative due to its high sensitivity. Besides, the response time and limit of detection are improved working in dynamic mode. On the other hand, a secondary antibody conjugated with gold nanoparticles has been used as a method to measure concentrations of antigens obtaining a great frequency shift. © 2012 The Authors. Published by Elsevier Ltd.


Matatagui D.,GRIDSEN | Fontecha J.,GRIDSEN | Fernandez M.J.,GRIDSEN | Oliver M.J.,University of Castilla - La Mancha | And 6 more authors.
Sensors and Actuators, B: Chemical | Year: 2013

A Love-wave device and a PDMS microfluidic chip have been developed in order to measure immunoreactions in liquid media, operating in dynamic mode (continuous flow-through). The obtained results with the above system for the detection of rabbit immunoglobulin by means of an antibody of goat anti-rabbit have been compared with those obtained by the most used acoustic wave device, the quartz crystal microbalance (QCM), which has worked in static mode. It has been demonstrated once again that QCM is an excellent tool as an immunosensor, although the Love-wave device is a good alternative due to its higher sensitivity. The dynamic mode improves the response time and the limit of detection. In addition, the use of microchannels has allowed working with a small sample volume for a long period of time in dynamic mode. On the other hand, a secondary antibody conjugated with gold nanoparticles has been used as a different method to measure the desired concentrations of antigens, obtaining in this case, a greater frequency shift. © 2013 Elsevier B.V.

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