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Saint Beauzire, France

Redouan E.,University of Picardie Jules Verne | Emmanuel P.,University of Picardie Jules Verne | Philippe M.,University Blaise Pascal | Bernard C.,University of Picardie Jules Verne | And 2 more authors.
Carbohydrate Polymers | Year: 2012

Glycosaminoglycan-like polysaccharides were prepared from Rhizobium sp. T1 polysaccharide using the TEMPO (nitroxyl radical 2,2,6,6-tetramethylpiperidine- 1-oxyl radical) mediated oxidation. The structure of this new polyglucuronic acid sodium salt was analyzed by 13C NMR spectra and HPAEC-PAD chromatography. Therefore, new polysaccharide containing only glucuronic acid monomers in both β-(1,3) and β-(1,4) linkage was obtained by the complete TEMPO-mediated oxidation of C6 primary hydroxyl groups of glucose of glucoglucuronan from Rhizobium sp. T1. Sulphation of this β-(1,3),β- (1,4)-polyglucuronic acid sodium salt was carried out using SO 3/DMF reagent. These results suggested a new synthetic route using both TEMPO-mediated oxidation and sulphation of polysaccharides from Rhizobium sp. in developing glycosaminoglycans mimic to enhance the profitability of its low-cost production and processing industries. This novel carbohydrate derivative might find use as cheaper surrogates of glycosaminoglycans in the cosmetics and pharmaceutical fields. © 2012 Elsevier Ltd. All rights reserved. Source


Saad N.,CNRS Chemistry of Natural Substances Laboratory | Delattre C.,GREENTECH Biotechnologies | Urdaci M.,French National Center for Scientific Research | Schmitter J.M.,University of Bordeaux 1 | And 2 more authors.
LWT - Food Science and Technology | Year: 2013

Probiotics and prebiotics play an important role in human nutrition. In recent years there has been a significant increase in research on the characterization and verification potential health benefits associated with the use of probiotic and prebiotic. The main effects attributed to selected probiotics/prebiotic products have been proved by clinical trials, while others have been acquired on the basis of in vitro tests which require in vivo transposition in order to be validated. The main clinical reports in the literature for the application of probiotic have been done for the treatment of infectious diseases including viral, bacterial or antibiotic associated diarrhoea, relief of chronic bowel inflammatory diseases, immuno-modulation, lowering of serum cholesterol, decreased risk of colon cancer, improve lactose digestion, reduce allergies, and effect on intestinal microbiota. Although the large investigation for the health benefits, information on probiotic species, a specific strain-therapeutic application, and sufficient dosages, is not sufficiently studied to allow practical and rational consumption. Moreover, prebiotic oligosaccharides although provided curative and nutritional values, they are poorly understood in regard to their origin, the processes employed to generate them, their fermentation profiles, and dosages required for health effects. The present review summarizes guidelines reported on the literature in regard to clinician or therapeutic trials of probiotic and prebiotic. © 2012 Elsevier Ltd. Source


Delattre C.,GREENTECH Biotechnologies | Michaud P.,University Blaise Pascal | Chaisemartin L.,GREENTECH Biotechnologies | Berthon J.Y.,GREENTECH Biotechnologies | Rios L.,GREENTECH Biotechnologies
Carbohydrate Polymers | Year: 2012

β-(1,3)-Polyglucuronic acid sodium salt was produced by the regioselective oxidation of curdlan using 2,2,6,6,-tetramethylpiperidine-1-oxyl radical (TEMPO)/NaBr/NaClO systems. This β-(1,3)-polyuronic acid sodium salt was depolymerized, O-sulphated and O-acetylated. All these molecules have been biologically screened for transcriptomic analysis using DNA array. The transcriptomical responses were studied after statistical gene ontology in order to evaluate biological responses disturbed by the individual treatments of human fibroblast by all these β-(1,3)-polyglucuronic acid sodium salt derivatives. © 2011 Elsevier Ltd. All rights reserved. Source


Delattre C.,GREENTECH Biotechnologies | Chaisemartin L.,GREENTECH Biotechnologies | Favre-Mercuret M.,GREENTECH Biotechnologies | Berthon J.Y.,GREENTECH Biotechnologies | Rios L.,GREENTECH Biotechnologies
Carbohydrate Polymers | Year: 2012

β-(1,3)-Polyglucuronic acid sodium salt produced by the regioselective oxidation of β-(1,3)-glucan have been studied for its biological impact as putative slimming agent. This β-(1,3)-polyglucuronic acid sodium salt was synthesized using the conventional 2,2,6,6,-tetramethylpiperidine-1-oxyl radical (TEMPO)/NaBr/NaClO systems at pH 10 and 4 °C. A transcriptomical study using DNA microarray analysis, demonstrated that this heparan like sodium salt locally induced an over-expression of the gene Angiopoietin-like 4 (ANGPTL4 or fasting induced adipose factor (FIAF)) leading to the increase in Adipokine ANGPTL4 synthesis and the inhibition of Lipoprotein Lipase (LPL). In vitro analysis using 3T3-L1 cells have clearly revealed that β-(1,3)- polyglucuronic acid sodium salt could act in key steps of lipid metabolism by inhibiting the differentiation of pre-adipocytes to mature adipocytes. © 2011 Elsevier Ltd. All Rights Reserved. Source

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