Port-Saint-Louis-du-Rhône, France


Port-Saint-Louis-du-Rhône, France
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Audoin C.,University of Nice Sophia Antipolis | Audoin C.,GREENSEA SAS | Bonhomme D.,University of Nice Sophia Antipolis | Ivanisevic J.,University of Nice Sophia Antipolis | And 4 more authors.
Marine Drugs | Year: 2013

The chemical investigation of the recently described Mediterranean Homoscleromorpha sponge Oscarella balibaloi revealed an original family of five closely related glucosylated sesterterpenes 1-4, named balibalosides. Their structure elucidation was mainly inferred from NMR and HRMS data analyses. Balibalosides differ by the pattern of acetyl substitutions on the three sugar residues linked to the same aglycone sesterterpenoid core. From a biosynthetic perspective, these compounds may represent intermediates in the pathways leading to more complex sesterterpenes frequently found in Dictyoceratida, a sponge Order belonging to Demospongiae, a clade which is phylogenetically distinct from the Homoscleromorpha. While steroid and triterpenoid saponins were already well known from marine sponges, balibalosides are the first examples of glycosilated sesterterpenes. © 2013 by the authors; licensee MDPI.

Agency: European Commission | Branch: FP7 | Program: CP-FP | Phase: KBBE.2010.3.2-01 | Award Amount: 4.34M | Year: 2011

Innovation is the most important engine of growth and jobs in knowledge-based bio-economies. The scope of BAMMBO (Biologically Active Molecules of Marine Based Origin) is ambitious. This is intentional. BAMMBO will provide innovative solutions to overcome existing bottle-necks associated with culturing marine organisms in order to sustainably produce high yields of value-added products for the pharmaceutical, cosmetic and industrial sectors. BAMMBO will screen and identify target marine organisms (e.g. bacteria, fungi, sponges, microalgae, macroalgae and yeasts) from diverse global locations for potential as sustainable producers of highadded value molecules (HVABs). Our project will apply analytical methods for the extraction, purification and enrichment of targeted bioactive compounds. A detailed life cycle analysis of the production pathways developed in the project will be undertaken to fully evaluate the sustainability of production of biologically active products from marine organisms. BAMMBO will exploit knowledge and technologies developed during the project and effectively manage their transfer to relevant stakeholders in industry and the research community, as well as to policy-makers. We have brought together a multidisciplinary consortium of specialist Research and SME partners representing 8 countries including partners from ICPC countries Russia and Brazil, and from EU member states at Mediterranean, Adriatic and Atlantic coasts. In adhering to the European Strategy for Marine and Maritime Research this three year project will encourage capacity-building, integration and synergies across relevant marine sectors. Innovative technologies developed in the project will be demonstrated with the involvement of industry partners, and the results will be of interest not only to companies directly involved in the marine sector, but to other large scale industry players such as pharmaceutical companies with interest in added-value bioactive compounds.

Audoin C.,University of Nice Sophia Antipolis | Audoin C.,GREENSEA SAS | Sanchez J.A.,University of Santiago de Compostela | Genta-Jouve G.,University of Paris Descartes | And 6 more authors.
Journal of Natural Products | Year: 2014

Filamentous cyanobacteria of the genus Phormidium have been rarely studied for their chemical diversity. For the first time, the cultivable Phormidium autumnale was shown to produce a prenylated cyclic peptide named autumnalamide (1). The structure of this peptide was fully determined after a deep exploration of the spectroscopic data, including NMR and HRMS. Interestingly, a prenyl moiety was located on the guanidine end of the arginine amino acid. The absolute configurations of most amino acids were assessed using enantioselective GC/MS analysis, with 13C NMR modeling being used for the determination of d-arginine and d-proline. The effects of 1 on sodium and calcium fluxes were studied in SH-SY5Y and hNav 1.6 HEK cells. When the Ca2+ influx was stimulated by thapsigargin, strong inhibition was observed in the presence of 1. As a consequence, this compound may act by disrupting the normal calcium uptake of this organelle, inducing the opening of the mitochondrial permeability transition pore, which results in the indirect blockade of store-operated channels. © 2014 The American Chemical Society and American Society of Pharmacognosy.

Audoin C.,University of Nice Sophia Antipolis | Audoin C.,GREENSEA SAS | Holderith S.,Direction Recherche and Technology | Romari K.,GREENSEA SAS | And 2 more authors.
Journal of Planar Chromatography - Modern TLC | Year: 2014

Although the different practical steps of a high-performance thinlayer chromatography (HPTLC) experiment are now all automated, this technique is suffering from the lack of computational treatment of the data. A large number of qualitative and quantitative protocols have been developed using HPTLC for the study of plants extracts. While for quantitative analyses ultraviolet (UV) detection is mainly used, qualitative comparisons of different samples are often realized by direct visualization of the plates. In this context, we developed the first work-flow dedicated to the analysis of HPTLC data, including the steps commonly used in metabolomics analyses. This development resulted in the increase of features detected and, more importantly, in the reduction of the common diffusion drift observed during HPTLC plate development. The output is a peak list containing RF values, intensities, and areas that can be used for statistical analyses. © Akadémiai Kiadó, Budapest.

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