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Port-Saint-Louis-du-Rhône, France

Audoin C.,University of Nice Sophia Antipolis | Audoin C.,GREENSEA SAS | Holderith S.,Direction Recherche and Technology | Romari K.,GREENSEA SAS | And 2 more authors.
Journal of Planar Chromatography - Modern TLC | Year: 2014

Although the different practical steps of a high-performance thinlayer chromatography (HPTLC) experiment are now all automated, this technique is suffering from the lack of computational treatment of the data. A large number of qualitative and quantitative protocols have been developed using HPTLC for the study of plants extracts. While for quantitative analyses ultraviolet (UV) detection is mainly used, qualitative comparisons of different samples are often realized by direct visualization of the plates. In this context, we developed the first work-flow dedicated to the analysis of HPTLC data, including the steps commonly used in metabolomics analyses. This development resulted in the increase of features detected and, more importantly, in the reduction of the common diffusion drift observed during HPTLC plate development. The output is a peak list containing RF values, intensities, and areas that can be used for statistical analyses. © Akadémiai Kiadó, Budapest.

Audoin C.,University of Nice Sophia Antipolis | Audoin C.,GREENSEA SAS | Sanchez J.A.,University of Santiago de Compostela | Genta-Jouve G.,University of Paris Descartes | And 6 more authors.
Journal of Natural Products | Year: 2014

Filamentous cyanobacteria of the genus Phormidium have been rarely studied for their chemical diversity. For the first time, the cultivable Phormidium autumnale was shown to produce a prenylated cyclic peptide named autumnalamide (1). The structure of this peptide was fully determined after a deep exploration of the spectroscopic data, including NMR and HRMS. Interestingly, a prenyl moiety was located on the guanidine end of the arginine amino acid. The absolute configurations of most amino acids were assessed using enantioselective GC/MS analysis, with 13C NMR modeling being used for the determination of d-arginine and d-proline. The effects of 1 on sodium and calcium fluxes were studied in SH-SY5Y and hNav 1.6 HEK cells. When the Ca2+ influx was stimulated by thapsigargin, strong inhibition was observed in the presence of 1. As a consequence, this compound may act by disrupting the normal calcium uptake of this organelle, inducing the opening of the mitochondrial permeability transition pore, which results in the indirect blockade of store-operated channels. © 2014 The American Chemical Society and American Society of Pharmacognosy.

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