Cuyacot A.R.,Sahmyook University |
Won S.Y.,South Korean National Institute of Animal Science |
Park S.K.,South Korean National Institute of Animal Science |
Sohn S.-H.,South Korean National Institute of Animal Science |
And 5 more authors.
Scientia Horticulturae | Year: 2016
The sequencing of Chrysanthemum boreale genome is now in progress. However, the repetitive DNA sequences that dominates greatly in the genome have not yet been elucidated in Chrysanthemum. Therefore, we performed fluorescence in situ hybridization (FISH) by using the mitotic metaphase chromosome of C. boreale with well-identified repeats and C0t DNA as probes. The most conserved repeats, rDNAs, as well as telomeric repeats were first observed. Three pairs of 45S rDNA sites were located at the termini of the short arms of chromosomes 3, 7, and 8, while one pair of 5S rDNA signals was observed in the middle of the long arm of chromosome 4. Arabidopsis -type telomeric repeats colocalizing with 45S rDNA sites, were detected at the ends of both chromosome arms. Next, on the basis of DNA reassociation kinetics, three types of C0t DNA that exhibited different degrees of repetitive nature were fractionated and used as FISH probes to map the repetitive sequences. Signals from all C0t DNAs were successfully observed, but their coverage on the chromosomes was different for C0t-1, C0t-10, and C0t-100. C0t-1 FISH signals showed intensity in the telomeric region and were dispersed on both chromosome arms, except for distal regions. C0t-10 signals were observed almost in all parts of the chromosome, with greater intensity around pericentromeric regions. Bright C0t-100 signals were observed throughout the chromosome excluding some distal parts. C0t FISH signals covered the rDNAs and telomeric repeats, thus signifying their repetitive attributes. The relative proportions of repetitive DNA sequences in C. boreale genome is discussed. © 2015 Elsevier B.V.
Kim S.H.,South Korean National Institute of Animal Science |
Choi Y.I.,Korea forest Research Institute |
Jin H.,Korea University |
Shin S.-J.,Chungbuk National University |
And 2 more authors.
Journal of Plant Biotechnology | Year: 2015
The isolation, cloning, and characterization of an R2R3-MYB transcription factor gene (MtMYB70) from the model legume Medicago truncatula is reported. MtMYB70 consists of a 768-bp coding sequence corresponding to 255 amino acids. Sequence alignment revealed that MtMYB70 cDNA contains conserved R2R3-type MYB domains with highly divergent C terminal regions. MtMYB70 was found to have relatively low sequence homology with known R2R3-MYB genes. Phylogenetic analysis placed the R2R3-MYB domain of MtMYB70 closest to PtMYB1, a known activator of lignin biosynthesis. Overexpression of MtMYB70 under the control of the 35S promoter in transgenic poplar did not cause a significant difference in total lignin content relative to the control, but glucan content was significantly increased in transgenic poplar. Therefore, MtMYB70 might have regulatory role in the biosynthesis of cell wall structural carbohydrates. © Korean Society for Plant Biotechnology.
Kim K.,Seoul National University |
Lee J.,Seoul National University |
Lee S.-C.,Seoul National University |
Kim N.-H.,Seoul National University |
And 5 more authors.
Mitochondrial DNA | Year: 2015
Eleutherococcus gracilistylus is a plant species that is close to E. senticosus, a famous medicinal plant called Siberian ginseng. The complete chloroplast genome sequence of the E. gracilistylus was determined by de novo assembly using whole genome next generation sequences. The chloroplast genome of E. gracilistylus was 156 770 bp long and showed distinct four partite structures such as a large single copy region of 86 729 bp, a small single copy region of 18 175 bp, and a pair of inverted repeat regions of 25 933 bp. The overall GC contents of the genome sequence were 36.8%. The chloroplast genome of E. gracilistylus contains 79 protein-coding sequences, 30 tRNA genes, and four rRNA genes. The phylogenetic analysis with the reported chloroplast genomes confirmed close taxonomical relationship of E. gracilistylus with E. senticosus. © 2015 Taylor & Francis.
Lee M.,Green Plant Institute |
Park J.,Green Plant Institute |
Lee H.,Green Plant Institute |
Sohn S.-H.,National Academy of Agricultural Science NAAS |
Lee J.,Green Plant Institute
Horticulture Environment and Biotechnology | Year: 2015
Citrus platymamma (Byeonggyul) of the family Rutacease is one of the most important genetic resources of Korean Citrus. To characterize the chloroplast (cp) genetic makeup of this species, the cp genome was sequenced by both Sanger and next generation sequencing (NGS) methods. The characterized cp genome is 160,121 bp in size: the large single copy (LSC) region is 87,732 bp, the inverted repeat (IR) is 26,998 bp, and the small single copy (SSC) region is 18,393 bp. The cp genome contains 114 genes and 21 introns, including 80 protein-coding genes, four RNA genes, 30 tRNA genes, 19 group II introns, and one group I intron. Based on phylogenetic analysis with six known cp genomes from the Sapindales order, to which the Rutaceae belongs, the cp genome of C. platymamma is closest to that of Citrus sinensis. When directly comparing the cp genomes of C. platymamma and C. sinenesis, we detected size variation in the psbC and rps4 genes, four SNPs, and eight 1-bp simple sequence repeats (SSRs). Additionally, we found that the cp genomic structures of C. platymamma and C. sinensis are identical in regards to the junction sequences of LSC-IRs and SSC-IRs, and provide evidence that the junction sequences of the SSC-IRs originated from the common ancestor of C. platymamma, C. sinensis, and C. aurantifolia. Further study of other species will provide enhanced understanding of cp genome evolution in the Citrus genus. © 2015, Korean Society for Horticultural Science and Springer-Verlag GmbH.