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Meguro-ku, Japan

Kasuya H.,Toho University | Hata E.,Toho University | Satou T.,Toho University | Yoshikawa M.,Hinoki Seikou Co. | And 3 more authors.
Natural Product Communications | Year: 2013

Various effects have been reported in the literature for the essential oil from Chamaecyparis obtusa (EOCO), such as antibacterial and antifungal activity. In this study, we examined the effect of EOCO on emotional behavior and stress-induced biomarkers. Male ICR mice, aged 5 weeks at the start of each experiment, were individually housed in cages for 1 week. After placing each mouse in a glass container and exposing it to EOCO for 90 min, we then investigated the influence on emotional behavior using the elevated-plus maze (EPM) test, which is one of the evaluation methods for anxiolytic-like behavior. Significant anxiolytic-like effects were observed for the 7.0 mg/L air EOCO (P<0.05). After the EPM test, mice were dissected and changes in the stressinduced biomarkers within the brain were investigated by examining the amounts of fast nerve growth factor receptor (NGFR) and activity regulated cytoskeletal-associated protein (Arc) gene expression, and brain-derived neurotrophic factor (BDNF) and galactokinase 1 (GLK1) protein expression. Significant increases were observed in the amount of NGFR after inhalation of 7.0 mg/L air EOCO (P<0.05). These results indicate that EOCO has both anxiolytic-like and stress mitigation effects. Source


Takahashi M.,Toho University | Satou T.,Toho University | Ohashi M.,Toho University | Hayashi S.,Green Flask Co. | And 2 more authors.
Natural Product Communications | Year: 2011

Essential oils have traditionally been used for decades to alleviate the symptoms of various mental problems. In terms of anxiolytic-like properties, lavender oil is probably the most commonly used and best-studied essential oil. Although there is compositional variance among the oils extracted from different origins, there have been few studies performed to date to investigate how these differences affect the expression of anxiolytic-like activity. This paper discusses the interspecies differences and contributions of the main constituents in the expression of anxiolytic-like effects upon inhalation, as assessed in mice by the elevated-plus maze test, by comparing the essential oils from six different species of Lavandula. The results showed qualitative and quantitative variations in the tested oils, which results in significant differences in their anxiolytic-like activities. Our findings also suggest that linalyl acetate (LA) works synergistically with linalool (LO) and that the presence of both LA and LO is essential for the whole oil to work as an inhaled anti-anxiety agent. Source


Satou T.,Toho University | Kasuya H.,Toho University | Takahashi M.,Toho University | Murakami S.,Toho University | And 3 more authors.
Flavour and Fragrance Journal | Year: 2011

Several reports have described the relationship between the quantity of an essential oil (EO), delivered by injection, and the effects on emotional behaviour. However, few studies have examined the relationship between the duration of EO inhalation and effects on emotional behaviour. It is necessary to clarify this relationship to accomplish effective utilization of an EO. Therefore, we aimed to clarify the relationship between EO inhalation time and anxiolytic-like effects. The anxiolytic-like effect of inhaled EO at various administration times was examined in normal healthy mice using the elevated-plus maze (EPM) test. The effect of a dose of 3.5mg/l air of Alpinia zerumbet EO was assessed using the EPM test. A time-dependent potentiation in the anxiolytic-like effect was observed when the inhalation time was altered from 0 to 120min. A significant (P<0.05 compared to time 0) anxiolytic-like effect was observed to peak at inhalation times of 90 and 120min. However, the anxiolytic-like effect returned to normal with a 150-min exposure. There was an optimal time of EO inhalation for expressing an anxiolytic-like effect. © 2011 John Wiley & Sons, Ltd. Source


Satou T.,Toho University | Matsuura M.,Toho University | Takahashi M.,Toho University | Umezu T.,Japan National Institute of Environmental Studies | And 3 more authors.
Flavour and Fragrance Journal | Year: 2011

It has been proposed that essential oil extracted from Abies sachalinensis (EOAS) has a relaxation effect, among others, in humans; however, the scientific evidence is currently insufficient. To clarify the influence of EOAS on emotional behaviour, we examined its anxiolytic-like effect using a mouse model. The elevated plus maze task was used to evaluate the anxiolytic-like effect. The results showed that intraperitoneal (i.p.) administration of EOAS at 0.6g/kg body weight did not produce an anxiolytic-like effect. However, a significant anxiolytic-like effect was observed after inhalation of EOAS at 3.6mg/l air (P<0.05). Next, the effect of the route of administration (i.p. vs. inhalation) on the distribution of the major components of EOAS was analysed. α-Pinene was observed in the brain at 5.7±2.0mg/kg (i.p.) and 19.7±0.9mg/kg (inhalation). The data showed that EOAS effectively produced an anxiolytic-like effect after administration by inhalation. Thus, the efficacy of EOAS in producing an anxiolytic-like effect is likely to be due to achieving adequate levels in the brain after EOAS had been inhaled. © 2011 John Wiley & Sons, Ltd. Source


Takahashi M.,Toho University | Yoshino A.,Toho University | Yamanaka A.,Toho University | Asanuma C.,Toho University | And 5 more authors.
Natural Product Communications | Year: 2012

Inhalation of various essential oils elicits behavioral changes as a consequence of a complex centrally coordinated response. To understand the molecular mechanisms of action of aromatic compounds on emotional responses, we evaluated the stress-induced changes in mouse brain and the efficacy of inhaled essential oil from Lavandula officinalis (LvEO) using two approaches: a behavioral test, and examining the expression levels of selected genes {fast nerve growth factor receptor (NGFR) mRNA, activity regulated cytoskeletal-associated protein (Arc) mRNA} and proteins {galactokinase 1 (GLK1) and brainderived neurotrophic factor (BDNF)}. Animals were randomly divided into 4 groups depending on the treatment given: stress (-)/H2O, stress (-)/LvEO, stress (+)/H2O, and stress (+)/LvEO group. For behavioral testing, using an elevated plus-maze test, significant anxiolytic-like effects were seen in both the stress (-)/LvEO and stress (+)/LvEO groups, indicating that LvEO exerts anxiolytic-like effects regardless of the administration of water immersion stress. On expression analysis, the levels of NGFR and Arc mRNA were significantly lower in animals subjected to stress. Inhalation of LvEO, however, reversed this change, thus suggesting that LvEO negates the impact of stress on gene expression levels. Meanwhile, significant decreases in expression levels were also observed in the stress (-)/LvEO group, which implies that LvEO, when given in a stress-free situation, may act as a stress stimulus. Taken together, our data suggest that inhalation of LvEO exerts bidirectional influences in the central nervous system (CNS) of animals, either attenuating the effects of stress or acting as a stressor, depending on the subject state. Source

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