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Bertin E.,Fondazione Instituto Of Ricerca Pediatrica Citta Della Speranza | Piccoli M.,Fondazione Instituto Of Ricerca Pediatrica Citta Della Speranza | Franzin C.,Fondazione Instituto Of Ricerca Pediatrica Citta Della Speranza | Nagy A.,Mount Sinai Hospital | And 3 more authors.
Journal of Visualized Experiments | Year: 2017

Induced pluripotent stem (iPS) cells are generated from mouse and human somatic cells by forced expression of defined transcription factors using different methods. Here, we produced iPS cells from mouse amniotic fluid cells, using a non-viral-based transposon system. All obtained iPS cell lines exhibited characteristics of pluripotent cells, including the ability to differentiate toward derivatives of all three germ layers in vitro and in vivo. This strategy opens up the possibility of using cells from diseased fetuses to develop new therapies for birth defects. © 2017 Journal of Visualized Experiments.

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