Grandhope Biotech Co.

Guangzhou, China

Grandhope Biotech Co.

Guangzhou, China
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Xu B.,Jinan University | Huang X.,Jinan University | Wei X.,Grandhope Biotech Co. | Zeng Y.,Jinan University
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology | Year: 2016

Objective To explore the effect of acellular extracellular matrix (ECM) transplantation on the innate immunity (including macrophages and neutrophils) of mice, and establish a method evaluating the immunogenicity of acellular ECM material in mice as experimental animals. Methods Fresh membrane material and newly-processed acellular ECM material without DNA were separately intraperitoneally transplanted into mice. Seven days later, samples were collected to measure spleen index. The absolute numbers of neutrophils in peripheral blood and macrophages in peritoneal fluid were determined by BD Trucount tubes. The concentrations of interleukin 2 (IL-2), IL-4, IL-6, interferon-γ (IFN-γ), tumor necrosis factor α (TNF-α), IL-17A and IL-10 in the plasma and peritoneal fluid were detected by BD(TM) Cytometric Bead Array (CBA) mouse Th1/Th2/Th17 cytokine kit. Results Compared with sham group, the body mass is not increased in the mice transplanted with fresh membrane material, but the spleen index increased. The fresh material could dramatically increase the numbers of neutrophils in peripheral blood and macrophages in peritoneal fluid, and increase IL-6 and IFN-γ production. However, acellular ECM material prepared by improved technique only had minor or even no effect on those parameters. Conclusion Acellular ECM material with DNA removed has no obvious response to the inherent immunity in mice.


Hu X.,Jinling Institute of Technology | Li D.,Grandhope Biotech Co. | Tan H.,Nanjing University of Science and Technology | Pan C.,Jinling Institute of Technology | Chen X.,Jinling Institute of Technology
Journal of Macromolecular Science, Part A: Pure and Applied Chemistry | Year: 2014

Injectable hydrogels have attracted a lot of attention in drug delivery, however, their capacity to deliver water-insoluble or hydrophobic anti-cancer drugs is limited. Here, we developed injectable graphene oxide/graphene composite supramolecular hydrogels to deliver anti-cancer drugs. Pluronic F-127 was used to stabilize graphene oxide (GO) and reduced graphene oxide (RGO) in solution, which was mixed with α-cyclodextrin (α-CD) solution to form hydrogels. Native hydrogel was used as control. GO or RGO slightly shortened gelation time. The storage and loss moduli of the hydrogels were tracked by dynamic force measurement. The storage modulus of GO or RGO composite hydrogels was larger than that of the native hydrogel. Hydrogels were unstable in solution and eroded gradually. GO or RGO in Pluronic F-127 solution could potentially improve the solubility of the water-insoluble anti-cancer drug camptothecin (CPT), especially with large drug-loaded CPT amount. Drug release behaviors from solutions and hydrogels were characterized. The nanocomponents (GO or RGO) were able to bind more drug molecules either for CPT or for doxorubicin hydrochloride (DXR) in solution. Therefore, GO or RGO composite hydrogel could potentially enable better controlled and gentler drug release (for both CPT and DXR) than native hydrogel. © 2014 Copyright Taylor & Francis Group, LLC.


Hu X.H.,Jinling Institute of Technology | Li D.,Grandhope Biotech Co.
Materials Technology | Year: 2013

Hydrogel contact lenses are ideal carriers for ophthalmic drug delivery. Conventional poly(hydroxyethyl methacrylate) hydrogel contact lenses have restricted applications in ophthalmic drug delivery due to low oxygen transmissibility. Water and hydrophilic monomer N-vinyl-2-pyrrolidone are introduced in polymerisation to enhance the properties of hydrogel such as gelation time, transparency, structure, swelling and water retention properties in this work. Norfloxacin as a drug model could be loaded into hydrogels by soaking repeatedly. The sustained drug release in a given period is dependent on the characteristics of solution and loading time. © 2013 W. S. Maney & Son Ltd.


Hu X.H.,Jinling Institute of Technology | Tan H.P.,Nanjing University of Science and Technology | Li D.,Grandhope Biotech Co. | Gu M.Y.,Jinling Institute of Technology
Materials Technology | Year: 2014

Soft contact lenses are very popular for vision correction and cosmetic alterations since 1960. However, wearing soft contact lenses induced many healthy problems, which are often related to the surface properties of contact lenses such as low wettability and protein deposition. Chitosan (CS)/Hyaluronic acid (HA) multilayer was assembled on the surface of contact lenses by layer-bylayer (LbL) technique to improve the surface properties of low wettability and protein deposition in the research. Surface modification improved the hydrophilic, reduced protein adsorption, enhanced water retention and increased the surface roughness length. Since LbL self-assembled multilayer films were proved to deliver drug effectively, norfloxacin and timolol as model drugs were loaded into CS/HA multilayer during LbL process. The contact lens with CS/HA multilayer could control norfloxacin steadily release in 1 h, timolol steadily release in half an hour. The novel aspect of the research is the construction of CS/HA multilayer on the surface of contact lenses for improving their surface properties and drug delivery. The impact of the research concerns the applications of multilayer and LBL technique, which opens up a useful way to decrease the problem of wearing hydrogel contact lenses and increase the possibility of hydrogel contact lenses as drug carrier. © 2014 W. S. Maney & Son Ltd.


Hu X.,Jinling Institute of Technology | Ma X.,Jinling Institute of Technology | Tan H.,Nanjing University of Science and Technology | Li D.,Grandhope Biotech Co.
Micro and Nano Letters | Year: 2013

Graphene as a two-dimensional material is particularly attractive because of its excellent electrical conductivity, mechanical properties, large surface area, low coefficient of thermal expansion and very high aspect ratio. However, the water insoluble property of graphene restricts its application in biomedical fields. Therefore the objective of this reported work is to find an efficient way to synthesise water-soluble and biocompatible graphene for biomedical applications. A stable aqueous graphene oxide (GO) solution was first obtained in the presence of non-ionic Pluronic copolymer. The GO-tripolymer showed good solubility in phosphate buffered saline (PBS) and the same UV absorption peaks as GO. Since a traditional reducing agent such as hydrazine had large toxicity, reduced GO (RGO) obtained by hydrazine reduction has some toxicity. In this work, a non-toxic reducing agent of ascorbic acid, galactose or bovine serum albumin was used as a RGO-tripolymer solution. The RGO-tripolymer exhibited good solubility in PBS. Finally, the cytotoxicity of RGO-tripolymers was investigated. Any RGO-tripolymer showed low cytotoxicity. © The Institution of Engineering and Technology 2013.


Patent
Grandhope Biotech Co. | Date: 2012-01-30

A nasal bridge implant is made according to a method that includes the steps of collecting animal material from a bovine or porcine source, the animal material being either a tendon or a ligament, removing cells from the animal material, shaping the animal material to provide a desired shape for the nasal bridge implant, crosslinking the animal material, removing antigens from the animal material, subjecting the animal material to an alkaline treatment, coupling into the animal material active substances which are capable of adhering growth factor and stem cell, and packing the animal material in a container that contains a sterilization solution.


Patent
Grandhope Biotech Co. | Date: 2012-01-27

A biological jawbone prosthesis is made according to a method that includes the steps of collecting animal material from a bovine or porcine source, the animal material being a jawbone, shaping the animal material to provide a desired shape for the jawbone implant, removing cells from the animal material, crosslinking the animal material, removing antigens from the animal material, subjecting the animal material to an alkaline treatment, coupling into the animal material active substances which are capable of adhering growth factor and stem cell, and packing the animal material in a container that contains a sterilization solution.


Patent
Grandhope Biotech Co. | Date: 2012-01-31

A cartilage prosthesis is made according to a method that includes the steps of collecting animal material from a bovine, ovine or porcine source, the animal material being a cartilage, shaping the animal material to provide a desired shape for the cartilage implant, removing cells from the animal material, crosslinking the animal material, removing antigens from the animal material, subjecting the animal material to an alkaline treatment, coupling into the animal material active substances which are capable of adhering growth factor and stem cell, and packing the animal material in a container that contains a sterilization solution.


PubMed | Grandhope Biotech Co., Jinan University and University of Science and Technology of China
Type: Journal Article | Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology | Year: 2016

Objective To explore the effect of acellular extracellular matrix (ECM) transplantation on the innate immunity (including macrophages and neutrophils) of mice, and establish a method evaluating the immunogenicity of acellular ECM material in mice as experimental animals. Methods Fresh membrane material and newly-processed acellular ECM material without DNA were separately intraperitoneally transplanted into mice. Seven days later, samples were collected to measure spleen index. The absolute numbers of neutrophils in peripheral blood and macrophages in peritoneal fluid were determined by BD Trucount tubes. The concentrations of interleukin 2 (IL-2), IL-4, IL-6, interferon- (IFN-), tumor necrosis factor (TNF-), IL-17A and IL-10 in the plasma and peritoneal fluid were detected by BD(TM) Cytometric Bead Array (CBA) mouse Th1/Th2/Th17 cytokine kit. Results Compared with sham group, the body mass is not increased in the mice transplanted with fresh membrane material, but the spleen index increased. The fresh material could dramatically increase the numbers of neutrophils in peripheral blood and macrophages in peritoneal fluid, and increase IL-6 and IFN- production. However, acellular ECM material prepared by improved technique only had minor or even no effect on those parameters. Conclusion Acellular ECM material with DNA removed has no obvious response to the inherent immunity in mice.


PubMed | Jinan University and Grandhope Biotech Co.
Type: | Journal: Materials science & engineering. C, Materials for biological applications | Year: 2014

Current therapy for skin wound healing still relies on skin transplantation. Many studies were done to try to find out ways to replace skin transplantation, but there is still no effective alternative therapy. In this study, decellularized scaffolds were prepared from pig peritoneum by a series of physical and chemical treatments, and scaffolds loaded with hyaluronic acid (HA) and epidermal growth factor (EGF) were tested for their effect on wound healing. MTT assay showed that EGF increased NIH3T3 cell viability and confirmed that EGF used in this study was biologically active in vitro. Scanning electron microscope (SEM) showed that HA stably attached to scaffolds even after soaking in PBS for 48 h. ELISA assay showed that HA increased the adsorption of EGF to scaffolds and sustained the release of EGF from scaffolds. Animal study showed that the wounds covered with scaffolds containing HA and EGF recovered best among all 4 groups and had wound healing rates of 49.86%, 70.94% and 87.41% respectively for days 10, 15 and 20 post-surgery compared to scaffolds alone with wound healing rates of 29.26%, 42.80% and 70.14%. In addition, the wounds covered with scaffolds containing EGF alone were smaller than no EGF scaffolds on days 10, 15 and 20 post-surgery. Hematoxylin-Eosin (HE) staining confirmed these results by showing that on days 10, 15 and 20 post-surgery, the thicker epidermis and dermis layers were observed in the wounds covered with scaffolds containing HA and EGF than scaffolds alone. In addition, the thicker epidermis and dermis layers were also observed in the wounds covered with scaffolds containing EGF than scaffolds alone. Skin appendages were observed on day 20 only in the wound covered with scaffolds containing HA and EGF. These results demonstrate that the scaffolds containing HA and EGF can enhance wound healing.

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