PubMed | University of Minnesota, Federal University of Santa Maria, Santa Catarina State University and Graduate Program in Animal Science
Type: | Journal: Experimental parasitology | Year: 2016
The aim of this study was to evaluate the effect of copper edetate on biochemical parameters, oxidative profile, cholinesterases activities, as well as its capacity to control gastrointestinal parasites in infected sheep. Thus, Lacaune sheep (n=18) infected by Haemonchus contortus were used and divided into three groups of six animal each: the group A was composed of untreated animals (the control group), the group B was formed by animals treated with 0.3mg/kg of copper edetate, and the group C was composed of animals treated with 0.5mg/kg of copper edetate. Blood collection was performed on days 0, 10, 20 and 30 after mineral supplementation and different variables were measured. Cholinergic system was evaluated to determine the acetylcholinesterase (AChE) activity in total blood and butyrylcholinesterase (BChE) activity in serum. Eggs per gram of feces (EPG) were evaluated. There were no significant differences (P>0.05) between groups regarding total protein, albumin, globulin and urea levels, GGT activity, as well as the hematocrit, and EPG. ALT activity decreased (P<0.05) on groups B and C on day 30 compared to the control group (the group A). AChE activity decreased (P<0.05) in the group C on days 10 and 30 compared to the control group, such decrease (P<0.05) was also observed for BChE activity in the group C on day 10. ROS levels increased in the group C compared to groups A and B on day 10, while the SOD activity increased in the group C on days 20 and 30 compared to the control group (P<0.05). CAT activity did not differ between groups (P>0.05). In summary, the copper edetate was not efficient to control gastrointestinal parasites, but efficiently activated SOD, an important antioxidant enzyme. In addition, copper edetate was able to partially inhibit cholinesterases activities when supplementated at its highest dose.
Abreu M.L.C.,Graduate Program in Animal Science |
Vieira R.A.M.,State University of Norte Fluminense |
Rocha N.S.,Graduate Program in Animal Science |
Araujo R.P.,Graduate Program in Animal Science |
And 4 more authors.
Asian-Australasian Journal of Animal Sciences | Year: 2014
Samples of Clitoria ternatea L. (Cunhã) were harvested at 35, 50, 70, and 90 d after a uniformity harvest in a field study designed as a completely randomized design with a total of 18 experimental plots. The dry matter yield of the whole plant was separated quantitatively into leaves, stems, and pods at each harvesting age. Chemical analyses and in vitro gas production kinetics were performed to assess the quality of the plant parts. Yields, chemical composition, and estimates of gas production parameters were analyzed by fitting a mixed statistical model with two types of covariance structures as follows: variance components and an unrestricted structure with heterogeneous variances. Fast and slow gas yielding pools were detected for both leaves and stems, but only a single pool was detected for pods. The homoscedasticity assumption was more likely for all variables, except for some parameters of the gas production kinetics of leaves and stems. There was no presence of typical pods at 35 and 50 d. In the leaves, the fibrous fractions were affected, whereas the non-fibrous fractions were unaffected by the harvesting age. The harvesting age affected the majority of the chemical constituents and gas kinetic parameters related to the stems. The leaves of this legume were the least affected part by the aging process. © 2014 by Asian-Australasian Journal of Animal Sciences.