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Vallabh Vidyanagar, India

Nakade D.B.,Government Rajaram College
Journal of Pure and Applied Microbiology

Water samples from different sites in Rankala lake of Kolhapur city, were analysed over a period of one year for actinomycetes population. Microscopic, cultural, biochemical characters,pigmentation,cell wall analysis and spore morphology and other criteria were used to identify the actinomycetes isolates.Members of genus Streptomyces were identified by MICRO-IS software Portyrata and Krichevsky (1992). Isolates other than Streptomyces genus were identified using Bergeys manual of systematic bacteriology by Williams et.,al.(1989). The actinomycetes species identified are Streptomyces (32),Streptoverticillum (28), kineosporia (23), Sporichthya (18), Streptosporangium (16),Micromonospora (17), Nocardia (14), Microbispora (11),Spirillospora (9), Microbispora (7), Dactylosporangium (6),Actinoplanes (5), Planomonospora(3), Kitasatosporia (1). Isolates were screened for antibacterial and antifungal activity by Agar overlay technique using two bacterial and four fungal species. Results indicated that the water from Rankala lake is rich in biodiversity of actinomycetes, 3 species showed antifungal activity and 13 showed antibacterial activity. It is the first study on microbiological aspect of Rankala lake. Present study indicated that the actinomycetes in Rankala Lake have a great potential to be an antibiotic producer and can be explored for antibiotic production. Source

Gokhale M.V.,P.A. College | Gokhale M.V.,Abhivyakti Academy for Research Education and Awareness AAREA | Shaikh S.S.,Shivaji University | Shaikh S.S.,Abhivyakti Academy for Research Education and Awareness AAREA | Toro S.V.,Government Rajaram College
International Journal of Botany

Pollen fertility status of 30 species including mangroves, mangrove associates as well as other coastal species from Maharashtra and Goa States of India is studied by using Alexander's Differential staining technique. Three different groups of plants are considered on the basis of the population size viz. large sized, medium sized and small sized populations including narrowly distributed species. The results obtained show there is slight decrease in the pollen fertility from large sized populations to small sized populations. Overall, pollen fertility of the studied species ranges from 53 to 98%. © 2012 Asian Network for Scientific Information. Source

Hake S.L.,JSM College | Chate P.A.,JSM College | Sathe D.J.,KITs Engineering College | Hankare P.P.,Shivaji University | Bhuse V.M.,Government Rajaram College
Journal of Materials Science: Materials in Electronics

We have successfully deposited cadmium selenide (CdSe) thin films by simple dip method using ascorbic acid as complexing agent. Variation of thickness with time and temperature were studied. Deposited samples were characterized by X-ray diffraction, scanning electron microscopy. The absorption, electrical and photoelectrochemical properties are also studied. The X-ray diffraction analysis shows that the film samples are in hexagonal structure. The optical band gap energy was found to be 1.70 eV. Activation energy was found to be 0.447 and 0.034 eV for higher temperature and lower temperature respectively. For CdSe photoelectrode, the open circuit voltage and short circuit current are found to be 267 mV and 175 mA respectively. The calculation shows the fill factor is 28.67 %. The power conversion efficiency is found to be 1.01 %. © 2013 Springer Science+Business Media New York. Source

Nakade D.B.,Government Rajaram College
Journal of Pure and Applied Microbiology

In the present study halotolerent indigenous strain of Azospirillum lipoferum N-29 was isolated on modified Congored Nitrogen free agar with 6% NaCl concentration from the hyper saline soils of Kolhapur district of Maharashtra, India. It was identified as by using Bergey's manual of systematic bacteriology Williams et, al.,(1989). To study its nitrogen fixing ability acetylene reduction assay was performed as per Dobereiner (1997), as well as a pot experiment was carried out on sugarcane 8071 variety, which is cultivated on large scale in western Maharashtra region of India. Nine pots divided in to three sets. Set-1 labelled as control set seeded with sugarcane setts without any treatment. Set-2 seeded with sugar cane setts soaked in Azospirillum lipoferum N-29 in sterile soil. Set-3 seeded with sugar cane setts soaked in Azospirillum lipoferum N-29 one set each pot in normal hyper saline soil. For production of biofertilizers a biomass production experiment was carried using fully automatic bioreactor, using a modified Nitrogen free broth medium at 30°C temperature,pH7.0, 140rpm agitation. Results indicated that Azospirillum lipoferum N-29 strain grows up to 8% NaCl concentration with optimum 4 % NaCl concentration, pH 7.0, temperature 30°C and at 140 rpm. It tolerates about 12% NaCl salt concentration for 1 hour. Nitrogen fixing ability Results indicated that there was maximum nitrogen fixation (22%) observed at 30°C temperature , 4% NaCl salt concentration, at 140 rpm agitation and at pH 7.0. Pot experiment showed that there was 58% increase in height of plant, diameter of stem, number of setts, and number of leaves as compared to control set and 20 % increase in sterile soil with Azospirillum lipoferum N-29, this indicated the combine effect of other microorganisms with that of Azospirillum lipoferum N-29.Biofertilizer production experiment indicated that maximum biomass (4*109 cells ml-1) can be produced within 48 hours at 30°C and at 140 rpm using modified Nitrogen free broth medium with 20% sucrose.My study indicated the suitability of Azospirillum lipoferum N-29 strain as a candidate for biofertilizer production for saline soils as well as for reclamation of saline soils. Source

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