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Moezzi M.,University of Pecs | Zapf I.,University of Pecs | Fekecs T.,Government Office of Baranya County | Nedvig K.,Zala County Hospital | And 2 more authors.
Journal of Thermal Analysis and Calorimetry | Year: 2016

Oxidative stress induced by oxygen free radicals (OFRs) is a casual factor in psoriasis. Our aim was to detect the oxidative stress parameters and blood plasma changes with differential scanning calorimetry (DSC) in psoriatic patients. The study included untreated (n = 39) and treated (retinoids, methotrexate, biologic response modifiers; n = 33) white adult patients from both sex. To monitor oxidative stress concentration of malondialdehyde (MDA), reduced glutathione and sulfhydryl groups, production of OFRs, and activity of myeloperoxidase (MPO), superoxide dismutase, and catalase were measured. Denaturation of plasma components was detected in SETARAM Micro DSC-II calorimeter. Total production of OFRs and MPO activity, and the concentration of MDA were significantly increased both in untreated patients with moderate and severe symptoms and in all drug-treated groups compared with controls (p < 0.001). All of the scavengers and antioxidants were significantly decreased in untreated patients and better preserved after retinoid and biological therapy. DSC scans of blood plasma showed melting temperature a characteristic parameter to follow the severity of disease. The calorimetric enthalpy is exhibiting a moderate decrease with the progression of the inflammation. These findings suggest that an imbalance exists between pro-oxidants and antioxidants in untreated severe psoriatic patients. All drug therapy reduced the changes, mainly the biologic response modifiers. Similarly, DSC showed differences between untreated and conventional systemic drug treatment. © 2015 Akadémiai Kiadó, Budapest, Hungary. Source


Zapf I.,University of Pecs | Moezzi M.,University of Pecs | Fekecs T.,Government Office of Baranya County | Nedvig K.,Zala County Hospital | And 2 more authors.
Journal of Thermal Analysis and Calorimetry | Year: 2016

Damage caused by oxidative stress is involved in many types of diseases, including breast cancer. Our aim was to detect the oxidative stress parameters and blood plasma changes with differential scanning calorimetry (DSC) in breast cancer patients. The study included 40 adult breast cancer women who were grouped according to tumor diameter, regional lymph node metastases, proliferative activity, receptor status and postoperative chemotherapy. To monitor oxidative stress, malondialdehyde, oxygen free radicals (OFRs), activity of myeloperoxidase (MPO), superoxide dismutase (SOD) and catalase (CAT) were measured. Denaturation of plasma components was detected in Setaram Micro DSC-II calorimeter. The total production of OFRs, the MPO activity and lipidperoxidation were significantly increased in each breast cancer patients considering the tumor size, the metastatic lymph nodes, the proliferation activity and receptor status compared with healthy controls (p < 0.05). These pro-oxidants were slightly elevated without chemotherapy, but their values were increased significantly in chemotherapy-receiving group. The activity of SOD and CAT was significantly decreased in all groups, and in regard to the chemotherapy, they were changed significantly parallel to the severity of disease. Regarding to both the increased tumor diameter and the increased number of affected lymph nodes, DSC measurements showed a strong relationship between the maximum excess heat capacity (C pmax) of the blood plasma and the severity of disease. The study demonstrated that oxidative stress is implicated in breast carcinoma and chemotherapy aggravates these changes which confirmed the plasma DSC measurements also. © 2015 Akadémiai Kiadó, Budapest, Hungary. Source

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