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Bhubaneshwar, India

Tripathy S.K.,Government of Odisha
International Journal of Theoretical Physics | Year: 2013

Anisotropic Locally Rotationally Symmetric Bianchi-I (LRSBI) cosmological model is investigated with variable gravitational and cosmological constants in the framework of Einstein's general relativity. The shear scalar is considered to be proportional to the expansion scalar. The dynamics of the anisotropic universe with variable G and Λ are discussed. Our calculations for the Supernova constraints concerning the luminosity distance provide reasonable results. © 2013 Springer Science+Business Media New York.

In the present study, we developed a set of three chimeric/hybrid promoters namely FSgt-PFlt, PFlt-UAS-2X and MSgt-PFlt incorporating different important domains of Figwort Mosaic Virus sub-genomic transcript promoter (FSgt, -270 to -60), Mirabilis Mosaic Virus sub-genomic transcript promoter (MSgt, -306 to -125) and Peanut Chlorotic Streak Caulimovirus full-length transcript promoter (PFlt-, -353 to +24 and PFlt-UAS, -353 to -49). We demonstrated that these chimeric/hybrid promoters can drive the expression of reporter genes in different plant species including tobacco, Arabidopsis, petunia, tomato and spinach. FSgt-PFlt, PFlt-UAS-2X and MSgt-PFlt promoters showed 4.2, 1.5 and 1.2 times stronger GUS activities compared to the activity of the CaMV35S promoter, respectively, in tobacco protoplasts. Protoplast-derived recombinant promoter driven GFP showed enhanced accumulation compared to that obtained under the CaMV35S promoter. FSgt-PFlt, PFlt-UAS-2X and MSgt-PFlt promoters showed 3.0, 1.3 and 1.0 times stronger activities than the activity of the CaMV35S2 (a modified version of the CaMV35S promoter with double enhancer domain) promoter, respectively, in tobacco (Nicotiana tabacum, var. Samsun NN). Alongside, we observed a fair correlation between recombinant promoter-driven GUS accumulation with the corresponding uidA-mRNA level in transgenic tobacco. Histochemical (X-gluc) staining of whole transgenic seedlings and fluorescence images of ImaGene Green™ treated floral parts expressing the GUS under the control of recombinant promoters also support above findings. Furthermore, we confirmed that these chimeric promoters are inducible in the presence of 150 μM salicylic acid (SA) and abscisic acid (ABA). Taken altogether, we propose that SA/ABA inducible chimeric/recombinant promoters could be used for strong expression of gene(s) of interest in crop plants.

Tripathy S.K.,Government of Odisha
Astrophysics and Space Science | Year: 2014

We study cosmological models with anisotropy in expansion rates in the context of the recent observations predicting an accelerating universe. In the absence of any anisotropy in the cosmic fluid, it is shown that the role of skewness in directional Hubble rates is crucial in deciding the behavior of the model. We find that incorporation of skewness leads to a more evolving effective equation of state parameter. © 2013 Springer Science+Business Media Dordrecht.

Sahu B.K.,Government of Odisha
Indian Journal of Environmental Protection | Year: 2015

Intensive agriculture has been practiced in some pockets of Angul-Talcher industrial region of Odisha. Though pesticides are indispensible for intensive agriculture, their indiscriminate use invites water quality problems. Pesticides contamination of ground water is a matter of great concern as ground water is used for drinking by rural agricultural households. Residues of some organochlorine pesticides, namely BHC, lindane, DDT, endosulphan, heptachlor, aldrin and dialdrin were monitored in ground water samples collected from 5 dug wells located near the agricultural fields in the study area. In spite of ban on use of most of the organochlorine pesticides, it is found from the study that the residues of some of these pesticides in dug well water are still higher than their maximum residue limit (MRL) recommended for drinking water. © 2015 - Kalpana Corporation.

Patro S.,Government of Odisha | Maiti I.B.,University of Kentucky | Dey N.,Government of Odisha
Journal of Biotechnology | Year: 2013

We have developed a novel bi-directional promoter (FsFfCBD) by placing two heterogeneous core-promoters from the Figwort mosaic virus sub-genomic transcript promoter (FsCP, -69 to +31) and Cauliflower mosaic virus 35S promoter (CCP, -89 to +1) respectively on upstream (5') and downstream (3') ends of a tri-hybrid enhancer (FsEFfECE), in reverse orientation. The FsEFfECE domain encompasses three heterologous enhancer fragments from Figwort mosaic virus sub-genomic transcript promoter (FsE, 101. bp, -70 to -170), Figwort mosaic virus full-length transcript promoter (FfE, 196. bp, -249 to -54) and Cauliflower mosaic virus 35S promoter (CE, 254. bp, -343 to -90). The bi-directional nature of the FsFfCBD promoter (coupled to GFP and GUS) was established both in transient systems (onion epidermal cells and tobacco protoplasts) and transgenic plant (Nicotiana tabacum samsun NN) by monitoring the simultaneous expression of GFP and GUS employing fluorescence (for GFP) and biochemical (for GUS) based assays. In transgenic plants, the FsFfCBD promoter was found to be 6.8 and 2.5 times stronger than two parent promoters; Fs and FfC respectively. The bi-directional compound promoter FsFfCBD, composed of three heterologous enhancers with enhanced activity could become a valuable additional tool for efficient plant metabolic engineering and molecular pharming. © 2012 Elsevier B.V.

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