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Farooq S.,Sher-e-Kashmir University of Agricultural Sciences and Technology | Wani S.A.,SKUAST of Kashmir | Hassan M.N.,Government of Jammu and Kashmir | Nazir N.,SKUAST of Kashmir | Nyrah Q.J.,SKUAST of Kashmir
Anaerobe | Year: 2015

In a study conducted, a total of 450 swab samples from footrot lesions of naturally infected sheep were collected in all the ten districts of the Kashmir valley and were examined for the presence of Dichelobacter nodosus (D. nodosus) and Fusobacterium necrophorum (F. necrophorum), in order to determine if F. necrophorum was associated with ovine footrot. The detection of F. necrophorum and D. nodosus was carried out by polymerase chain reaction targeting the leukotoxin (lktA) and 16S rRNA genes, respectively. In this study, only less than 50% of positive samples contained both the bacteria, so it is not possible to conclude with certainty that both bacteria are together required for the disease manifestation. © 2015 Elsevier Ltd.

Hussain M.I.,Sher-e-Kashmir University of Agricultural Sciences and Technology | Wani S.A.,Sher-e-Kashmir University of Agricultural Sciences and Technology | Nagamani K.,Gandhi Medical College and Hospital | Fayaz I.,Sher-e-Kashmir University of Agricultural Sciences and Technology | And 5 more authors.
World Journal of Microbiology and Biotechnology | Year: 2010

Seven hundred and thirty-five diarrhoeic faecal samples from children were investigated for presence of enteroaggregative E. coli (EAEC), enterotoxigenic E. coli (ETEC), diffusely adherent E. coli (DAEC) and Salmonella spp. by polymerase chain reaction (PCR) and bacterial culture. Out of 675 samples from Kashmir, 55 isolates were obtained, which carried at least one virulence gene studied. Out of the 55 isolates, 36 (65.45%) were EAEC, 18 (32.72%) were ETEC while only one isolate (1.81%) was DAEC. All the EAEC isolates were found to be typical as they possessed aggR gene. Six (16.66%) EAEC isolates carried the astA gene. Out of the 18 ETEC isolates, 13 carried the elt gene alone, four possessed both the elt and est genes and the remaining one harboured the est gene alone. Five ETEC isolates also possessed astA gene. Nineteen EAEC isolates belonged to 10 different serogroups. Serogroup O153 was most frequent. The ETEC isolates also belonged to 10 different serogroups of which O159 was most predominant. Out of 224 E. coli isolates from 60 samples of Secunderabad, 27 isolates carried at least one virulence gene. Out of 27 isolates 22 (81.48%) were typical EAEC, three (11.11%) were ETEC and two (7.4%) were DAEC. Fifteen EAEC isolates belonged to seven different serogroups with O86 as most frequent. Four EAEC isolates also possessed the astA gene. All the three ETEC isolates harboured elt gene only and belonged to three different serogroups. Two isolates of Salmonella Worthington were obtained from only two samples in Kashmir. © 2010 Springer Science+Business Media B.V.

Bhat S.A.,Barkatullah University | Chalkoo S.R.,Government of Jammu and Kashmir | Shammi Q.J.,Barkatullah University
Turkish Journal of Fisheries and Aquatic Sciences | Year: 2011

Fish were offered either restricted feeding (5% of body weight or 1% body weight as maintenance requirement) or appetite feeding subdivided into three equal feeds. The best (P<0.05) growth response in terms of final body weight, percent weight gain and specific growth rate (SGR) was observed for feeding schedule I (control), for fish fed to appetite throughout. Feeding schedule II showed significantly the highest (P<0.05) growth response in terms of final body weight, percent weight gain and specific growth rate (SGR) in comparison with other feeding schedules. Growth rate and food conversion efficiency (FCE) in feeding schedule II were markedly higher during phase II in comparison with phase I. Phase I (0-4 weeks) growth rate slightly increased in fish fed restricted ration throughout (T-II). In contrast, in phase II (4 - 8 weeks) fish were fed to appetite throughout (T-II) showed a rapid increase in fortnightly growth response and higher growth rates, feed consumption and FCE. © Published by Central Fisheries Research Institute (CFRI) Trabzon, Turkey.

Khan A.M.,Guru Angad Dev Veterinary and Animal Sciences University | Sultana M.,Sher-e-Kashmir University of Agricultural Sciences and Technology | Raina R.,Sher-e-Kashmir University of Agricultural Sciences and Technology | Dubey N.,Government of Jammu and Kashmir | Dar S.A.,Sher-e-Kashmir University of Agricultural Sciences and Technology
Proceedings of the National Academy of Sciences India Section B - Biological Sciences | Year: 2013

The current study was undertaken to investigate the potential oxidative stress and hematological alterations induced by sub-acute oral exposure of bifenthrin in goats, after its repeated oral administration, at the dose rate of 5 mg/kg body weight daily for 28 days. The oxidative stress parameters such as malondialdehyde, blood glutathione, superoxide dismutase, catalase, glutathione peroxidase and glutathione s-transferase were studied on days 0, 3, 7, 14, 21 and 28 of bifenthrin exposure and days 7 and 14 of post-exposure period. To explore the relationship of oxidative stress with the alterations in hematological parameters like hemoglobin, packed cell volume and total erythrocyte count were also studied, simultaneously. The bifenthrin-exposed animals showed a significant increase in malondialdehyde level, whereas, the significant decrease in the levels of antioxidant parameters was noticed. A significant decrease in hemoglobin, packed cell volume and total erythrocyte count was reported, indicating a close relationship of oxidative stress and hematological alterations. After bifenthrin withdrawal, there was an improvement in the condition, as indicated by reversal in both oxidative stress and hematological parameters to the normal range. © 2013 The National Academy of Sciences, India.

Muzaffar S.,University Of Kashmir | Maqbool K.,University Of Kashmir | Wani I.A.,University Of Kashmir | Masoodi F.A.,University Of Kashmir | Bhat M.M.,Government of Jammu and Kashmir
Acta Alimentaria | Year: 2016

Studies were conducted to characterize the chestnut starch for physico-chemical properties. Chemical composition of chestnut starch showed low levels of protein and ash indicating purity of starch. The results revealed low water and oil absorption capacity of chestnut starch. Starch showed high swelling power and low solubility index. Swelling power and solubility index of chestnut starch increased with increase in temperature (50-90 °C). The results revealed high initial, peak, setback, breakdown, and fi nal viscosity but low paste development temperature. Transmittance (%) of the starch gel was low and decreased with increasing storage period. The chestnut starch gel showed increase in % water release (syneresis) with increase in time of storage but was less susceptible to repeated cycles of freezing and thawing. Starch was also characterized for granule morphology. Starch granules were of round and oval shapes, some granules showed irregular shape. © 2016 Akadémiai Kiadó, Budapest.

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