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Naik M.I.,Government Motilal Vigyan Mahavidyalya | Tenguria R.K.,Government Motilal Vigyan Mahavidyalya | Haq E.,University Of Kashmir
Journal of Helminthology | Year: 2016

Human cystic echinococcosis (CE), caused by Echinococcus granulosus, is one of the most important and widespread parasitic zoonoses. One of the problems that can be encountered after treating CE patients is the risk of post-surgical relapses or treatment failure, thus a long-term clinical and serological follow-up is required to evaluate the success or failure of therapy. In the present study immunological markers have been identified to indicate the effectiveness of pharmacological and surgical treatments. The relationship between serum cytokine levels and the outcome of chemotherapy and surgery was evaluated in 50 patients with CE. Serum interleukin (IL)-4, IL-10 and interferon-gamma (IFN-γ) concentrations were determined by enzyme-linked immunosorbent assay (ELISA) before and after pharmacological and surgical treatment. Serum cytokine levels of IL-4, IL-10 and IFN-γ were elevated in a significant proportion of patients during the active stage of disease. IL-4, IL-10 and IFN-γ were measurable in 41 (82%), 37 (74%) and 25 (50%) patients before the treatment. Clinical and radiological assessment of patients 2 years after pharmacological treatment has shown that 48 of 50 patients responded to treatment. IL-4 and IL-10 levels were decreased significantly (P < 0.05) in these patients. Conversely, patients who did not respond showed high levels of IL-4 and IL-10 and undetectable levels of IFN-γ. Hence these results suggest that serum IL-4 and IL-10 detection may be useful in the follow-up of patients with CE. Copyright © Cambridge University Press 2015.

Naik M.I.,Government Motilal Vigyan Mahavidyalya | Tenguria R.K.,Government Motilal Vigyan Mahavidyalya | Haq E.,University Of Kashmir
Helminthologia (Poland) | Year: 2015

An enzyme linked immunosorbent assay (ELISA), based on sheep hydatid cyst fluid antigen was used for the detection of specific antibodies of IgG, IgM, IgE and IgG subclass in the serum samples of 62 clinically and radiologically diagnosed cystic echinococcosis (CE) patients, 8 clinically suspected cases of CE, 25 other parasitic disease controls and 25 healthy controls. The diagnostic sensitivity in the clinically and radiologically suggestive cases (n = 62) for IgG antibody detection was highest (93 %), followed by IgE, IgG4, IgG1, IgG2, IgM and IgG3 with 89 %, 87 %, 85 %, 76 %, 70 % and 55 % respectively. The detection of specific IgE, IgG1 and IgG4 antibody showed the higher diagnostic sensitivity and specificity to the extent that they can be safely used as better substitute to IgG. Even though, the diagnostic sensitivity of IgG was highest (93%) but was less specific (88 %) due to the frequent non-specific reactions in the sera of patients with other parasitic infections and healthy controls. None of the sera samples from healthy controls gave non-specific reaction with IgE, IgG1 and IgG4 and there was a considerably reduced cross-reaction with these antibodies. The most discriminatory and specific antibodies found in this study belonged to IgE, IgG1 and IgG4; therefore, these antibodies may serve as useful diagnostic markers for CE. © Institute of Parasitology, SAS, Košice 2015.

Kumar Tenguria R.,Government Motilal Vigyan Mahavidyalya | Naik M.I.,Government Motilal Vigyan Mahavidyalya | Fomda B.,Sher i Kashmir Institute of Medical science
Iranian Journal of Public Health | Year: 2013

Background: Cystic echinococcosis (CE) is one of the most important parasitic zoonosis worldwide. Due to the high recurrence rate of the disease after surgery, follow up of the patient is necessary. The aim of current research was to assess the performance of Western blotting (WB), using sheep hydatid fluid, for serological diagnosis and post-treatment monitoring of human CE. Methods: Serum samples obtained from 50 clinically/radiologically proven cases of CE along with serum samples from non-CE patients and healthy persons were tested by WB, using sheep hydatid fluid as antigen. Results: The WB test enabled the detection of antibodies in the pre-operative samples for proteins of 18-239 kDa in molecular weight. From 50 sera of CE patients, 31 cases (72.09%) detected 52 kDa subunit, 27 cases (62.79%) detected 24 kDa band, 26 cases (60.46%) recognised 39 kDa band and 21 cases (48.83%) identified 46 kDa component of sheep hydatid antigen. Sera from patients with other parasitic infections and malignancy showed cross-reactivity with the cluster of 54-59 kDa bands. The healthy control sera were not reactive to any antigenic fraction. The antigenic bands with molecular weight of 52, 24, 39 and 46 kDa were specific for CE, and may serve as useful diagnostic markers. The antibodies specific to proteins 24 and 39 kDa significantly decreased in the patients cured after surgery, while in patients with recurrent parasitism the bands present before surgery persisted. Conclusion: The WB with sheep hydatid antigen might be useful in the diagnosis and post-surgical monitoring of CE patients.

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