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Dasari S.,Dravidian University | Shouri R.N.D.,Government General Hospital | Rajendra W.,Sri Venkateswara University | Valluru L.,Dravidian University
Biomedicine and Pharmacotherapy | Year: 2014

Objectives: To evaluate the effectiveness of radiochemotherapy and chemotherapy on human papilloma virus induced cervical cancer patients by the estimation of serum proteins and magnetic resonance imaging. Methods: HPV 16/18 viral DNA was detected in the plasma of cervical cancer patients (n= 50) by PCR using HPV consensus primers. Of the 50cervical cancer patients, 25cases undergoing radiation with chemotherapy and another 25cases undergoing chemotherapy. Levels of pre- and post-treated serum squamous cell carcinoma antigen, soluble CD44, cancer antigen-125 were measured and evaluated the tumour size at pre- and post-radiation based on magnetic resonance images. The effectiveness of treatment was evaluated in terms of protein levels and represented as whisker line graphs. Results: Of the amplified 50samples, HPV 16 and 18 strains were identified as 48 and 44%, respectively. Serum protein levels were significantly increased in both pre-treated groups when compared to healthy group. Post-treated (radiotherapy) cervical cancer patients' shows decreased tumour size when compared to pre-treated groups. Taking consideration of proteins, squamous cell carcinoma antigen, soluble CD44, cancer antigen-125 levels are more decreased in patients treated with radiochemotherapy than chemotherapy alone. The decreased levels of proteins were significantly higher in early stage of the cervical cancer than the advanced stage of cancer patients. Conclusion: Serum levels of protein markers are more improved in patients treated with radiochemotherapy than chemotherapy hence, radiochemotherapy may be the best choice of treatment with reference to proteins at early stage of cervical cancer when compared to chemotherapy alone. © 2014 Elsevier Masson SAS.

Dasari S.,Dravidian University | Shouri R.N.D.,Government General Hospital | Wudayagiri R.,Sri Venkateswara University | Valluru L.,Dravidian University
Asian Pacific Journal of Tropical Disease | Year: 2014

Objective: To assess the probiotic nature of Lactobacillus in preventing cervical pathogens by studying the effectiveness of antimicrobial activity against vaginal pathogens. Methods: Lactobacilli were isolated from healthy vaginal swabs on selective media and different pathogenic bacteria were isolated by using different selective media. The Lactobacillus strains were tested for the production of hydrogen peroxide and antimicrobial compounds along with probiotic properties. Results: Of the 10 isolated Lactobacillus strains, strain 1, 3 and 6 are high hydrogen peroxide producers and the rest were low producers. Results of pH and amines tests indicated that pH increased with fishy odour in the vaginal fluids of cervicovaginal infection patients when compared with vaginal fluids of healthy persons. The isolates were found to be facultative anaerobic, Gram-positive, non-spore-forming, non-capsule forming and catalase-negative bacilli. The results of antimicrobial activity of compounds indicated that 280 and 140 μg/mL was the minimum concentration to inhibit the growth of both pathogens and test organisms respectively. Conclusions: The results demonstrated that Lactobacillus producing antimicrobial compounds inhibits the growth of cervical pathogens, revealing that the hypothesis of preventing vaginal infection by administering probiotic organisms has a great appeal to patients, which colonize the vagina to help, restore and maintain healthy vagina. © 2014 Asian Pacific Tropical Medicine Press.

Supriya Simon A.,Pushpagiri Institute of Medical science | Dinesh Roy D.,Center for Advanced Genetic Studies | Jayapal V.,Government General Hospital | Vijayakumar T.,Kannur University
Indian Journal of Clinical Biochemistry | Year: 2011

Cardiovascular autonomic neuropathy (CAN) is one of the most clinically significant complications of diabetes mellitus. Even though many ethological factors have been attributed for the pathogenesis of this disease no attempts were made to correlate DNA damage as a causative factor. Hence the present study was undertaken to asses the extent of somatic DNA damages by cytokinesis-block micronuclei assay (CBMN). An attempt is also being made to correlate the habits and/or risk factors and socioeconomic status with CAN. The CBMN frequency of 46 patients suffering from autonomic neuropathy was compared with that of 25 healthy age and sex matched controls. All the subjects were suffering from type 2 diabetes for at least 8 years and have varying degrees of coronary artery diseases. The mean CBMN frequency of the patients was statistically higher than that of the healthy control subjects (P < 0.05). The CBMN frequency was found to be significantly altered in CAN patients who where physical inactivity and smoking. A significant correlation could also be observed between CAN and smoking, diabetes mellitus, hypertension, dyslipidemia, abdominal obesity, and physical activity. © 2010 Association of Clinical Biochemists of India.

Venugopal M.,Guntur Medical College | Renuka I.V.,Guntur Medical College | Saila Bala G.,Guntur Medical College | Seshaiah N.,Government General Hospital
Journal of Oral and Maxillofacial Pathology | Year: 2013

Malignant melanoma of the oral cavity is a rare lesion, with an incidence of about 0.2% to 0.8% of all melanomas. Melanoma of tongue is still rarer and represents less than 2% of oro-nasal melanoma cases. We report a rare case of amelanotic melanoma of the tongue in a young man. The importance of consideration of melanoma in the differential diagnosis of oral cavity lesions is discussed since mucosal melanoma carries a bad prognosis and early diagnosis is vital.

Ravikumar B.,Government General Hospital | Lakshminarayana S.,Andhra University
Journal of Medical Physics | Year: 2012

In Brachytherapy treatment planning, the effects of tissue heterogeneities are commonly neglected due to lack of accurate, general and fast three-dimensional (3D) dose-computational algorithms. In performing dose calculations, it is assumed that the tumor and surrounding tissues constitute a uniform, homogeneous medium equivalent to water. In the recent past, three-dimensional computed tomography (3D-CT) based treatment planning for Brachytherapy applications has been popularly adopted. However, most of the current commercially available planning systems do not provide the heterogeneity corrections for Brachytherapy dosimetry. In the present study, we have measured and quantified the impact of inhomogeneity caused by different tissues with a 0.015 cc ion chamber. Measurements were carried out in wax phantom which was employed to measure the heterogeneity. Iridium-192 ( 192Ir) source from high dose rate (HDR) Brachytherapy machine was used as the radiation source. The reduction of dose due to tissue inhomogeneity was measured as the ratio of dose measured with different types of inhomogeneity (bone, spleen, liver, muscle and lung) to dose measured with homogeneous medium for different distances. It was observed that different tissues attenuate differently, with bone tissue showing maximum attenuation value and lung tissue resulting minimum value and rest of the tissues giving values lying in between those of bone and lung. It was also found that inhomogeneity at short distance is considerably more than that at larger distances.

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