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Singh R.,Guru Nanak Institutions | Sathe V.,University Campus | Kaur S.,Government College for Girls | Saini G.S.S.,Panjab University
Journal of Molecular Structure | Year: 2016

Effect of solvation on coumarin6 dye has been studied with density functional theory (DFT). Optimized structure of the dye has been obtained in various solvents and frequencies of various vibrational bands have been calculated in these solvents. Calculations predict shift in the frequency of certain bands in the solvents. Similar shifts have been observed experimentally in the vibrational spectra of the dye in solvents. In order to ascertain the origin of these shifts, the interactions of solvent molecules with the coumarin6 molecule have been studied using various tools of DFT like donor-acceptor interactions, Molecular Electrostatic potential (MEP) and HOMO-LUMO analysis etc. © 2015 Elsevier B.V. All rights reserved. Source


Singh D.P.,Government of Punjab | Singh D.P.,Panjab University | Khare P.,Government of Punjab | Khare P.,Panjab University | And 7 more authors.
International Journal of Obesity | Year: 2016

Background:High-fat diets (HFDs) induce systemic inflammation, gut microbial derangements and disturb metabolic homeostasis, resulting in weight gain, insulin resistance and nonalcoholic fatty liver (NAFL). Numerous antioxidants and prebiotic/probiotics per se may prevent HFD-associated comorbidities, but there are no reports related to their combination.Objective:In the present study, we aim to evaluate a cobiotic combination of lycopene (antioxidant) and isomalto-oligosaccharides (IMOs, a prebiotic) for prevention of HFD-induced alterations.Design:Male Swiss albino mice were fed either normal pellet diet (NPD) or HFD and lycopene (5 and 10 mg kg -1), IMOs (0.5 and 1 g kg -1) or their combination for 12 weeks. Systemic adiposity, glucose tolerance, insulin sensitivity, feeding regulators in hypothalamus, hepatosteatosis and liver inflammation, cecal short chain fatty acids (SCFAs), serum inflammatory cytokines, gut morphology and alterations in selected gut microbes were studied.Results:Lycopene, IMOs and their combination prevented weight gain, adiposity, improved adipose tissue fat mobilization and reduced insulin resistance. Hypothalamic orexigenic and anorectic genes have also been modulated by these treatments. Dietary interventions prevented NAFL-like symptoms and improved glucose homeostasis. Improvement in selected gut microbial abundance and SCFA concentration along with reduced systemic inflammation, metabolic endotoxemia and improved ileal and colonic health were observed in mice supplemented with lycopene, IMOs and their combination. Interestingly, cobiotic combination synergistically improved many of the HFD-induced alterations.Conclusion:The present work provide evidence that new approach based on cobiotic combination (antioxidant plus prebiotic) can be employed to develop novel class of functional foods for their application against HFD-associated pathological complications. © 2016 Macmillan Publishers Limited. Source


Saini G.S.S.,Panjab University | Dogra S.D.,Panjab University | Singh G.,Panjab University | Tripathi S.K.,Panjab University | And 3 more authors.
Vibrational Spectroscopy | Year: 2012

We report the effects of methanol, pyridine, diethylamine, dichloromethane, acetonitrile, bromine and NO 2 vapours on the Raman and infrared absorption spectra of zinc tetraphenylporphine thin films by recording their spectra before and after exposure with chemical vapours. Positions of some vibrational bands show detectable change on exposure. Changes in the intensity of some vibrational bands of the thin films have also been observed on exposure. Coordination of vapours molecules at the zinc ion and subsequent charge transfer are responsible for the shift in the vibrational bands. Density functional theory calculations have been carried out to determine the probable geometric structures of the porphyrin-vapour complexes. Calculated geometric structures show in-plane and out-of-plane distortions in the porphyrin macrocycle. Calculations also result in charge transfer between vapour and porphyrin molecules. © 2012 Elsevier B.V. Source


Sharma R.,Government College for Girls | Kaur J.,PGIMER | Chauhan S.S.,Panjab University | Mahmood A.,Panjab University
Molecular and Cellular Biochemistry | Year: 2011

The effect of alloxan-induced gestational diabetes on the postnatal development of brush border disaccharidases and d-glucose transport in rat intestine was studied. Pups born to diabetic mothers showed 92-22% increase in blood sugar levels compared with the controls. Western blot and RT-PCR analyses revealed that the activities of brush border sucrase, lactase and Sodium Glucose Co-transporter 1 (SGLT1) correlates with protein and mRNA levels in intestine of pups born to diabetic rat mothers after 5-45 days of birth. Intestinal histology in pups born to diabetic mothers at day 10 and 45 after birth showed distorted cellular organization of mucosa with a decrease in the number of secretary goblet cells and regression of tubular mass. These findings suggest that the genetic switch in utero regulates the postnatal expression of enzyme and transport functions in intestine of pups born to diabetic rat mothers. This may influence the growth and development of offsprings later in life. © 2011 Springer Science+Business Media, LLC. Source


Singh S.,Government College for Girls | Saini G.S.S.,Panjab University | Tripathi S.K.,Panjab University
Sensors and Actuators, B: Chemical | Year: 2014

The vacuum evaporated thin films of zinc phthalocyanine (ZnPc) were prepared at room temperature and structurally investigated using X-ray diffraction and scanning electron microscope (SEM). The temperature dependence of dark and photoconductivity of as-deposited (i.e. unexposed) and exposed ZnPc films were measured in the temperature range 305-389 K. Some of the optical absorption such as molar extinction coefficient, the oscillator strength, the electrical dipole strength have been evaluated. The optical band gaps of the ZnPc thin films have been determined from α2 versus hν graph. These films have been studied as chemical sensors such as methanol and ammonia. © 2014 Elsevier B.V. Source

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