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Safronova V.I.,All Russia Research Institute for Agricultural Microbiology ARRIAM | Kimeklis A.K.,All Russia Research Institute for Agricultural Microbiology ARRIAM | Chizhevskaya E.P.,All Russia Research Institute for Agricultural Microbiology ARRIAM | Belimov A.A.,All Russia Research Institute for Agricultural Microbiology ARRIAM | And 5 more authors.
Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology | Year: 2014

Sixteen bacterial strains were isolated from root nodules of Vavilovia formosa plants originated from the North Ossetian State Natural Reserve (Caucasus, Russia). Phylogenetic analysis of these strains was performed using partial 16S rRNA gene and internally transcribed spacer (ITS) sequences. The results showed that the isolates belong to three families of root nodule bacteria. Twelve of them were related to the genus Rhizobium (family Rhizobiaceae) but four strains can be most probably identified as Phyllobacterium-related (family Phyllobacteriaceae), Bosea- and Rhodopseudomonas-related (family Bradyrhizobiaceae). Amplified fragment length polymorphism clustering was congruent with ITS phylogeny but displayed more variability for Rhizobium isolates, which formed a single group at the level of 30 % similarity. We expect that the isolates obtained can belong to new taxa at genus, species or subspecies levels. The results of PCR amplification of the nodulation genes nodC and nodX showed their presence in all Rhizobium isolates and one Rhodopseudomonas-related isolate. The nodC gene sequences of V. formosa isolates were closely related to those of the species Rhizobium leguminosarum bv. viciae but formed separate clusters and did not intermingle with any reference strains. The presence of the nodX gene, which is necessary for nodulation of Afghan peas (Pisum sativum L.) originated from the Middle East, allows the speculation that these wild-type pea cultivars may be the closest existing relatives of V. formosa. Thus, the studies of genetic diversity and symbiotic genes of V. formosa microsymbionts provide the primary information about their phylogeny and contribute to the conservation of this relict leguminous species. © 2013 Springer Science+Business Media Dordrecht.


Temiraev V.K.,Gorsky State Agrarian University | Kairov V.R.,Gorsky State Agrarian University | Kalagova R.V.,North Ossetian State Medical Academy
Biology and Medicine | Year: 2014

In recent years, use of mycotoxins and antioxidants sorbents in cattle breeding is of considerable interest. Feeding these sorbents makes it possible to improve digestion process, metabolism, and productivity of animals, and improve product quality and economic performance. To this end, the aim of the research was to determine feasibility and advisability of treating fattening young cattle with a combination of biologically active preparations as a biological stimulant in order to improve their physiological and biochemical status and, consequently, to increase indicators of nutrients conversion into food products, fattening and meatiness, and profitability. Studies have shown that simultaneous presence of the ehpophen antioxidant and the toxisorb sorbent in rations of fattening young cattle stimulates physiological and biochemical processes in the organism, contributes to growth intensity, and reduces forage consumption per unit of output.


Safronova V.I.,All Russia Research Institute for Agricultural Microbiology ARRIAM | Kuznetsova I.G.,All Russia Research Institute for Agricultural Microbiology ARRIAM | Sazanova A.L.,All Russia Research Institute for Agricultural Microbiology ARRIAM | Kimeklis A.K.,All Russia Research Institute for Agricultural Microbiology ARRIAM | And 8 more authors.
Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology | Year: 2015

The Gram–negative, rod-shaped slow-growing strains Vaf-17, Vaf-18T and Vaf-43 were isolated from the nodules of Vavilovia formosa plants growing in the hard-to-reach mountainous region of the North Ossetian State Natural Reserve (north Caucasus, Russian Federation). The sequencing of 16S rDNA (rrs), ITS region and five housekeeping genes (atpD, dnaK, recA, gyrB and rpoB) showed that the isolated strains were most closely related to the species Bosea lathyri (class Alphaproteobacteria, family Bradyrhizobiaceae) which was described for isolates from root nodules of Lathyrus latifolius. However the sequence similarity between the isolated strains and the type strain B. lathyri LMG 26379T for the ITS region was 90 % and for the housekeeping genes it was ranged from 92 to 95 %. All phylogenetic trees, except for the rrs-dendrogram showed that the isolates from V. formosa formed well-separated clusters within the Bosea group. Differences in phenotypic properties of the B. lathyri type strain and the isolates from V. formosa were studied using the microassay system GENIII MicroPlate BioLog. Whole-cell fatty acid analysis showed that the strains Vaf-17, Vaf-18T and Vaf-43 had notable amounts of C16:0 (4.8–6.0 %), C16:0 3-OH (6.4–6.6 %), C16:1 ω5c (8.8–9.0 %), C17:0 cyclo (13.5–13.9 %), C18:1 ω7c (43.4–45.4 %), C19:0 cyclo ω8c (10.5–12.6 %) and Summed Feature (SF) 3 (6.4–8.0 %). The DNA–DNA relatedness between the strains Vaf-18T and B. lathyri LMG 26379T was 24.0 %. On the basis of genotypic and phenotypic analysis a new species Bosea vaviloviae sp. nov. (type strain RCAM 02129T = LMG 28367T = Vaf-18T) is proposed. © 2015, Springer International Publishing Switzerland.


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