Vladikavkaz, Russia

Gorsky State Agrarian University

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Kokaeva M.G.,North Caucasian Institute of Mining and Metallurgy | Temiraev R.B.,North Caucasian Institute of Mining and Metallurgy | Tsugkieva V.B.,Gorsky State Agrarian University | Dzhaboeva A.S.,Kabardino-Balkarian State University | And 2 more authors.
Journal of Pharmaceutical Sciences and Research | Year: 2017

The violation of the storage technology of feed crops results in the accumulation of nitrates, nitrites and mycotoxins. The purpose of research is to develop a method for improving the ecological and consumer properties of the milk of lactation cows and its derivative products by the rational use of the antioxidant etoxin and Mold-Zap in the diets containing the subtoxic dose of nitrates and aflatoxin B1. Methods. During the experiment using the method of pairs-analogues, we formed 4 groups of cows. The experiment material was processed by means of the Excel software from the Microsoft Office package. Results. Adding to the diet of the 3rd experimental group etoxin in a dose of 0.5 kg/ton and Mold-Zap in a dose of 0.5 kg/ton of compound animal feeding stuff showed a stimulating impact on the hematopoietic function of the cows of the 3rd experimental group due to which their blood contained more erythrocytes and hemoglobin in comparison with the control group with the simultaneous decrease in the concentration of methemoglobin. The ammonia content in the blood of the cows of the 3rd experimental group was 20.9% higher and the content of nitrates and nitrites, on the contrary, was 2.42 and 2.91 times lower. The enrichment of the cow diets with these preparations had a positive impact on the chemical composition of milk and that resulted in the decrease in the concentration of nitrates by 45.1%, nitrites by 55.3%, and aflatoxin M1 by 48.9%. The analogues of the 3rd experimental group showed the increase in the share of a-casein and the diameter of the casein micelles and this means the improvement of the milk suitability for cheese. The cheese sample made from the milk of the animals of the 3rd experimental group showed the lowest concentration of nitrates - 68.5% and nitrites - 70.0%. The content of aflatoxin M1 was 60.0% lower than in the control group. The level of nitrates, nitrites and aflatoxin in the cheese samples made from the milk of the cows of this group was lower than the maximum permissible concentrations. Field of application. The environment protection and rational natural resource management. © 2017, Pharmainfo Publications. All rights reserved.


Temiraev V.K.,Gorsky State Agrarian University | Kairov V.R.,Gorsky State Agrarian University | Temiraev R.B.,Gorsky State Agrarian University | Kubatieva Z.A.,Gorsky State Agrarian University | Gukezhev V.M.,Kabardino-Balkarian State University
Ecology, Environment and Conservation | Year: 2017

Modern poultry industry, striving to abandon the use of feed antibiotics, sees it especially urgent to apply natural stimulators of poultry growth to obtain environmentally safe products. Such poultry growth stimulators are probiotics. The methodology of the conducted studies is based on scientific concepts described in the papers of national researchers in this area, and the strict observation of tested zoocultural, physiological and biochemical study methods. In experiments, both common and special methods to process digital materials have been employed, based on statistical and mathematical analysis methods using the Microsoft Office software. Ross-308 cross broiler chickens were the target of the research. Each experiment included 4 groups, 1 control group and 3 test groups. The study results show that it is the first time in the Northern Caucasus that the practicability of feeding hulless barley grains after ozonation and the bifidumbacterinum probiotic is justified as applicable to the composition of broiler chickens of barley-wheat-sunflower type with a tolerant level of anatoxins A,. The studies revealed that feeding barley grains subjected to ozone along with the bifidumbacterinum probiotic to reduce the risk of aflatoxicosis in broiler chickens improved the survival rate and growth energy of poultry stock, and optimized the conversion of nutrients of the diet into the products. The combined additives of treated barley grains and probiotic had a higher effect on digestive exchange processes during the studies, which manifested itself in test group 3 chickens, as compared to control groups, in a higher digestibility index for dry and organic components of the diet, wet protein, crude fiber and nitrogen-free extractive substances. New data, describing the effect of these feed factors onto meat production performance, nutritional and biological value of meat, the level of digestive and intermediate exchange of broiler chickens, make a great contribution to zootechnics. The study results have been used to develop methodological recommendations for using fodders with a tolerant level of anatoxins to feed agricultural poultry, and are used for education purposes when training veterinarians. © EM International.


Gogaev O.K.,Gorsky State Agrarian University | Kessaev K.E.,Gorsky State Agrarian University | Kaloev B.S.,Gorsky State Agrarian University | Kebekov M.E.,Gorsky State Agrarian University | Tarchokov T.T.,Kabardino-Balkarian State University
Asian Journal of Microbiology, Biotechnology and Environmental Sciences | Year: 2016

Background of the problem/Goals: The study of the formation of the skin structure and hair coat that has not only a general biological but also a big practical value, especially for sheep of the Romanov breed, is a part of the cognition problem of the regularity of the individual development of animals. Method: The sampling of skin was carried out by means of a special tool (selecting knife) with a diameter of 2 cm, section area 3.14 cm2. The selecting knife allows selecting the skin samples with minimal harm for the animal and less labour input. The preparation of the skin drugs and their research was performed according to the method of Diomidovaet al. Results: It was revealed that in postembryonal period there is a further growth and differentiation of the skin structure, regularity and rate of which depends upon the age. The highest rate of the skin growth and formation of hair fibers from the embryo hair follicles of the Romanov sheep is during the first months of life and they stop by the age of 9 months. The other insignificant changes are related to the conditions of the season, feeding and growing. The formation of the hair fibers from the embryo hair follicles stops by the age of 9 months. The hair of the Romanov sheep consists of beard hair and fur hair. At birth, the beard hair exceeds the length of the fur hair 2.7-3.0 times and at the age of 6 months, on the contrary, the fur hair exceeds the beard hair 1.7-1.8 times. The average thickness of the main types of fibers increased with time and at the same time the fur hairs had the biggest thickening (growth factor 2.53), the average thickness of the beard hairs increased 1.7 times. As a result of the more intensive growth of length and thickness of the fur hairs, the sharp change of their percentage with age occurred. If the specific weight of the fur hairs of newborn lambs was 11.1%, at the age of 6 months it grew up to 73.2%. Field of application/Improvements: The conducted research allows to have a scientific grounding for the correct development of the measures directed to the improvement of the sheep breeding production of outputs. © Global Science Publications.


Tsugkiev B.G.,Gorsky State Agrarian University | Kabisov R.G.,Gorsky State Agrarian University | Tsugkieva V.B.,Gorsky State Agrarian University | Rekhviashvili E.I.,Gorsky State Agrarian University | Bittirov A.M.,Kabardino-Balkarian State University
International Journal of Pharmacy and Technology | Year: 2016

Background / Objectives: The search for new promising microbial strains is a topical and preferred direction for the development of various food industry branches. Thereby this article is aimed at finding domestic competitive starter cultures. Method: The main approaches to the study of this problem are to analyze the diversity of lactic microorganisms and select the best of them and that can be achieved by studying the complex of their cultural, physiological and biochemical and technological properties. Currently, when there is an intensive development of biotechnology in food production, including functional products, it is indisputably necessary to search for and develop scientific approaches to the production of probiotic food using physiologically active strains of lactic microorganisms that can inhibit the evolution in the body of pathogenic microorganisms and opportunistic pathogens as well as to improve the accessibility of ration ingredients and normalize micro flora of intestinal tract. Results: The article presents the results for selection of master seed microbial strains selected in various parts of the Republic of North Ossetia – Alania, including in the high mountain regions. It was found that lactic bacteria are widespread in the Republic of North Ossetia - Alania. It is conclusively established that the strains selected in the regions in which they are used are more likely to adapt to domestic raw materials and have a positive effect on the development of the microorganism; this became the basis for seeking master seed microbial strains in North Ossetia-Alania. The scientific value of the studies undertook in this area is that high active strains of lactobacillus were selected, deposited in the Russian Collection of Industrial Microorganisms of the State Research Institute of Genetics (GosNIIGenetika), and are used in practice in formulation of starter cultures in those regions of North Ossetia-Alania, where the microorganisms are not subjected to mutative changes as a result of human impact. A number of issues related to the identification and selection of starter cultures with high and standing potential, studying their biology and processing properties, the creation of functional products, which have an important role in human life, were considered. Motivation for the use of biologically-active probiotics in the diet of farm animals and poultry was scientifically established and proved. Application/Improvements: The information contained in this article serves a useful purpose for the dairy industry, poultry and livestock enterprises aimed at the expansion of the range of functional foods and the use of probiotic additives. © 2016, International Journal of Pharmacy and Technology. All rights reserved.


Safronova V.I.,All Russia Research Institute for Agricultural Microbiology ARRIAM | Kimeklis A.K.,All Russia Research Institute for Agricultural Microbiology ARRIAM | Chizhevskaya E.P.,All Russia Research Institute for Agricultural Microbiology ARRIAM | Belimov A.A.,All Russia Research Institute for Agricultural Microbiology ARRIAM | And 5 more authors.
Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology | Year: 2014

Sixteen bacterial strains were isolated from root nodules of Vavilovia formosa plants originated from the North Ossetian State Natural Reserve (Caucasus, Russia). Phylogenetic analysis of these strains was performed using partial 16S rRNA gene and internally transcribed spacer (ITS) sequences. The results showed that the isolates belong to three families of root nodule bacteria. Twelve of them were related to the genus Rhizobium (family Rhizobiaceae) but four strains can be most probably identified as Phyllobacterium-related (family Phyllobacteriaceae), Bosea- and Rhodopseudomonas-related (family Bradyrhizobiaceae). Amplified fragment length polymorphism clustering was congruent with ITS phylogeny but displayed more variability for Rhizobium isolates, which formed a single group at the level of 30 % similarity. We expect that the isolates obtained can belong to new taxa at genus, species or subspecies levels. The results of PCR amplification of the nodulation genes nodC and nodX showed their presence in all Rhizobium isolates and one Rhodopseudomonas-related isolate. The nodC gene sequences of V. formosa isolates were closely related to those of the species Rhizobium leguminosarum bv. viciae but formed separate clusters and did not intermingle with any reference strains. The presence of the nodX gene, which is necessary for nodulation of Afghan peas (Pisum sativum L.) originated from the Middle East, allows the speculation that these wild-type pea cultivars may be the closest existing relatives of V. formosa. Thus, the studies of genetic diversity and symbiotic genes of V. formosa microsymbionts provide the primary information about their phylogeny and contribute to the conservation of this relict leguminous species. © 2013 Springer Science+Business Media Dordrecht.


Temiraev V.K.,Gorsky State Agrarian University | Kairov V.R.,Gorsky State Agrarian University | Kalagova R.V.,North Ossetian State Medical Academy
Biology and Medicine | Year: 2014

In recent years, use of mycotoxins and antioxidants sorbents in cattle breeding is of considerable interest. Feeding these sorbents makes it possible to improve digestion process, metabolism, and productivity of animals, and improve product quality and economic performance. To this end, the aim of the research was to determine feasibility and advisability of treating fattening young cattle with a combination of biologically active preparations as a biological stimulant in order to improve their physiological and biochemical status and, consequently, to increase indicators of nutrients conversion into food products, fattening and meatiness, and profitability. Studies have shown that simultaneous presence of the ehpophen antioxidant and the toxisorb sorbent in rations of fattening young cattle stimulates physiological and biochemical processes in the organism, contributes to growth intensity, and reduces forage consumption per unit of output.


Safronova V.I.,All Russia Research Institute for Agricultural Microbiology ARRIAM | Kuznetsova I.G.,All Russia Research Institute for Agricultural Microbiology ARRIAM | Sazanova A.L.,All Russia Research Institute for Agricultural Microbiology ARRIAM | Kimeklis A.K.,All Russia Research Institute for Agricultural Microbiology ARRIAM | And 8 more authors.
Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology | Year: 2015

The Gram–negative, rod-shaped slow-growing strains Vaf-17, Vaf-18T and Vaf-43 were isolated from the nodules of Vavilovia formosa plants growing in the hard-to-reach mountainous region of the North Ossetian State Natural Reserve (north Caucasus, Russian Federation). The sequencing of 16S rDNA (rrs), ITS region and five housekeeping genes (atpD, dnaK, recA, gyrB and rpoB) showed that the isolated strains were most closely related to the species Bosea lathyri (class Alphaproteobacteria, family Bradyrhizobiaceae) which was described for isolates from root nodules of Lathyrus latifolius. However the sequence similarity between the isolated strains and the type strain B. lathyri LMG 26379T for the ITS region was 90 % and for the housekeeping genes it was ranged from 92 to 95 %. All phylogenetic trees, except for the rrs-dendrogram showed that the isolates from V. formosa formed well-separated clusters within the Bosea group. Differences in phenotypic properties of the B. lathyri type strain and the isolates from V. formosa were studied using the microassay system GENIII MicroPlate BioLog. Whole-cell fatty acid analysis showed that the strains Vaf-17, Vaf-18T and Vaf-43 had notable amounts of C16:0 (4.8–6.0 %), C16:0 3-OH (6.4–6.6 %), C16:1 ω5c (8.8–9.0 %), C17:0 cyclo (13.5–13.9 %), C18:1 ω7c (43.4–45.4 %), C19:0 cyclo ω8c (10.5–12.6 %) and Summed Feature (SF) 3 (6.4–8.0 %). The DNA–DNA relatedness between the strains Vaf-18T and B. lathyri LMG 26379T was 24.0 %. On the basis of genotypic and phenotypic analysis a new species Bosea vaviloviae sp. nov. (type strain RCAM 02129T = LMG 28367T = Vaf-18T) is proposed. © 2015, Springer International Publishing Switzerland.


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