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Song H.-T.,Jiangsu University | Guo W.,2102 Hospital of PLA | Sun X.-Y.,Shanghai University | Zhang L.,Jiangsu University | And 6 more authors.
Medical Journal of Chinese People's Liberation Army | Year: 2014

Objective To explore the correlation between the microRNA expression in plasma and psychiatric symptoms in schizophrenia patients. Methods A total of 53 consecutive schizophrenia patients who had not received antipsychotic treatment from July 2012 to May 2013 were enrolled in the present study. The expressions of 9 schizophrenia-associated microRNAs (miR- 30e, miR-34a, miR-181b, miR-195, miR-346, miR-432, miR-7, miR-132 and miR-212) in plasma were determined by real-time fluorescence quantitative PCR, with the psychiatric symptoms evaluated by Positive and Negative Symptoms Scale (PANSS), and the results were analyzed statistically. Results The expression level of miRNA-34a was significantly negatively correlated with the active symptoms and aggressive symptoms (r=-0.345, r=-0.284, P<0.05), and the expression level of miRNA-346 was significantly negatively correlated with the general psychopathologic symptoms (r=-0.282, P<0.05). The scores of activity and aggressiveness in the higher expression subgroup of miRNA-34a were significantly lower than those in lower expression subgroup (P<0.05). The score of disturbance of thought in the higher expression subgroup of miRNA-346 was significantly lower than that in lower expression subgroup (P<0.05). When miRNA-34a was entered into the two regression equations with the activity and aggressiveness as the independent variables respectively, it explained 10.2% of the variance of activity and 6.3% of the variance of aggressiveness.

Fan H.-M.,Cadre Ward | Fan H.-M.,Shanghai University | Sun X.-Y.,Shanghai University | Sun X.-Y.,Prevention and Treatment Center for Psychological Diseases | And 8 more authors.
Journal of Molecular Neuroscience | Year: 2015

Schizophrenia (SZ) is a debilitating psychotic disorder of unknown etiology, and the diagnosis is essentially based on clinical symptoms. So it is urgent to find an objective and feasible clinical diagnostic index for SZ. MicroRNA array was performed in peripheral blood mononuclear cells (PBMCs) obtained from young SZ patients and gender-, age-, and ethnicity-matched healthy controls. Then, real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to verify the top 10 microRNAs (miRNAs) with the highest fold change values in 55 SZ patients and 28 healthy controls, and 9 miRNAs demonstrate significant differences in expression levels (P < 0.01). Receiver operating characteristic (ROC) curve analysis showed that the combining area under the ROC curve (AUC) of the nine miRNAs was 0.973 (95 % confidence interval (CI): 0.945–1.000). miRNA target gene prediction and functional annotation analysis showed that there were significant enrichments in several gene ontology (GO) biological process and Kyoto encyclopedia of genes and genomes (KEGG) pathways associated with nervous system and brain functions, suggesting that the differentially expressed miRNAs may be involved in mechanism of SZ. We conclude that altered expression of miRNAs in PMBCs might be involved in young SZ pathogenesis and may serve as noninvasive biomarker for SZ diagnosis. © 2015, Springer Science+Business Media New York.

Sun X.-Y.,Prevention and Treatment Center for Psychological Diseases | Song H.-T.,Bengbu Medical College | Zhao L.,Guangji Hospital | Niu W.,102 Hospital of PLA | And 3 more authors.
Medical Journal of Chinese People's Liberation Army | Year: 2014

Objective To observe the changes in microRNA (miRNA) expression levels in peripheral blood of schizophrenia patients before and after treatment with antipsychotics. Methods Sixty-one consecutive patients with schizophrenia (case group) and 62 normal controls (control group) hospitalized to the 102nd Hospital of PLA from July 2012 to May 2013 were involved in this study. The relative expression levels of 9 miRNAs (miR-181b, miR-195, miR-132, miR-212, miR-30e, miR-346, miR-34a, miR-432, miR-7) in the peripheral blood plasma of patients in two groups were determined by real-time fluorescence quantitative PCR. Twenty-five schizophrenia patients with total score of Positive and Negative Syndrome Scale (PANSS) >70 were selected to determine the miRNA expression levels before and 3 and 6 weeks after antipsychotics (including olanzapine, quetiapine, ziprasidone and risperidone) treatment, and the clinical symptoms and treatment effect in different stages of therapy were assessed by PANSS, Global Assessment Scale (GAS), and Clinical Global Impression scale (CGI). Results The expression levels of miR-181b, miR-30e, miR-346, miR-34a and miR-7 in case group were significantly higher than those in control group (P<0.05, P<0.01). In the 25 patients with PANSS >70, the expression level of miR-132 lowered 3 weeks after treatment (P<0.05), the expression levels of miR-132, miR-181b, miR-30e and miR-432 lowered 6 weeks after treatment (P<0.05, P<0.01) compared with those before treatment. After treatment for 6 weeks, all the expression levels of the 9 \miRNAs in 25 patients were similar to those in control group (P>0.05). The expression of miR-132, miR-195, miR-30e and miR-432 were significantly correlated with the PANSS total score and GAS score along with the treatment course (P<0.05). Conclusion The miR-181b, miR-132, miR-30e and miR-432 maybe used as biological markers for the prediction of the prognosis of patients with schizophrenia. © 2014, People's Military Medical Press. All rights reserved.

Chen S.-D.,Shanghai University | Chen S.-D.,Chinese Peoples Liberation Army | Sun X.-Y.,PingAn Health Cloud Co. of China | Niu W.,Chinese Peoples Liberation Army | And 8 more authors.
Comprehensive Psychiatry | Year: 2016

This study investigated the correlation between the level of microRNA expression in peripheral blood mononuclear cells (PBMCs) and symptomatology in patients with generalized anxiety disorder (GAD). MicroRNA array was performed in peripheral blood mononuclear cells (PBMCs) obtained from GAD patients with gender, age, ethnicity-matched healthy controls. Then real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to verify the top 7 miRNAs with the highest fold-change values in 76 GAD patients and 39 healthy controls. It demonstrated that 5 miRNAs showed significantly differences in expression levels (P < 0.01). These 5 GAD-associated miRNAs were finally selected into our study to analyze the association between the plasma level of miRNAs expression and symptomatology scores in Hamilton Anxiety Scale (HAMA). Results showed that the level of miR-4505 and miR-663 was negatively correlated with the total HAMA scores in GAD patients (r = 0.2228, r = 0.264 P < 0.05). MiR-663 was selected into the regression equation of HAMA total scores and psychic anxiety symptomatology scores, and it could explain 5.3% of the HAMA total scores and 15.3% of the anxiety symptomatology scores. This study analyzed preliminarily possible circulating miRNAs expression changes in GAD patients, and the expression level of miR-663 highly correlated with psychic anxiety symptoms, further molecular mechanism of which needs to be explored. © 2016 Elsevier Inc.

Zhang Q.-L.,Xuzhou Medical College | Lu J.,GoPath Laboratories LLC | Sun X.-Y.,Prevention and Treatment Center for Psychological Diseases | Sun X.-Y.,Shanghai University | And 8 more authors.
European Journal of Psychiatry | Year: 2014

Background and Objectives: Currently, there is a serious need to find practical biomarker(s) for Major Depressive Disorder (MDD) therapeutic target(s). This study aimed to investigate the association between microRNA (miRNA, miR) expression level in Peripheral Blood Mononuclear Cells (PBMCs) and symptomatology improvement in MDD patients before and after six-week antidepressant treatment. Methods: By using an Affymetrix array that covers 723 human miRNAs, 26 miRNAs were identified with significantly altered expression in PBMCs in MDD patients, of which 10 miRNAs were selected for quantitative real-time Reverse Transcription Polymerase Chain Reaction (RT-PCR) study. Twenty out of all the 81 MDD patients were selected for miRNA expression levels testing and symptomatology assessments before and after sixweek treatment. Results: Compared with the control group, the expression levels of miR-26b, miR- 4743, miR-4498, miR-4485 and miR-1972 of the MDD group were significantly higher (P < 0.05); the changes of expression levels of miR-4743, miR-4498, miR-4485 and miR- 1972 were positively related to retardation improvement (P < 0.05), and the change of expression level of miR-26b negatively to the improvement of day and night change (P < 0.05); regression analysis result demonstrated that the alteration of miR-4485 expression accounted for 28.8% of retardation improvement (P < 0.05). Conclusions: These five miRNAs (miR-4743, miR-4498, miR-4485, miR-1972 and miR-26b) may serve as biomarker for MDD diagnosis and therapeutic targets for MDD treatment. © 2014, University of Zaragoza. All rights reserved.

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