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Fang D.,Jiaotong University | Bao Y.,Jiaotong University | Li X.,Gongli Hospital of Pudong New Area | Liu F.,Jiaotong University | And 3 more authors.
Chemotherapy | Year: 2010

Background and Aim: Multidrug resistance (MDR) compromises the efficacy of chemotherapy. Many approaches have been used to reduce MDR; however, the results are poor. It has been reported that iron deprivation downregulates MDR genes. To investigate the relationship of iron with MDR and early growth response gene-1 (EGR1), we investigated the effect of iron deprivation on expression and/or function of multidrug resistance-1 (MDR1), early growth response gene-1 (EGR1), ferritin heavy chain gene (H-Fn) and MDR1-encoded P-glycoprotein (P-gp) in the K562 leukemic cell line. Methods: The cells were stimulated with 12-O-tetradecanoylphorbol-13-acetate (TPA) and incubated with either FeCl 3 or the iron-chelating drug DFO. The mRNA levels of MDR1, EGR1 and H-Fn were detected by RT-PCR. The protein expression and function of P-gp were measured by immunohistochemical staining and flow cytometry, respectively. Results: DFO significantly reduced the intracellular iron level, and led to ∼70% reduction of MDR1 mRNA, ∼50% of reduction of H-Fn mRNA and ∼30% reduction of P-gp protein in TPA-differentiated K562 cells. The P-gp pump function, measured by daunorubicin exclusion, was also reduced by DFO treatment. Conclusions: These results suggest a close relationship between iron deprivation and reduced MDR1/P-gp expression and function. DFO may be used together with chemotherapeutic drugs to achieve better clinical efficacy. © 2010 S. Karger AG, Basel. Source


Wang Y.,Gongli Hospital of Pudong New Area | Zhao X.,Gongli Hospital of Pudong New Area | Huojia M.,Peoples Hospital of the Xinjiang Uygur Autonomous Region | Xu H.,Peoples Hospital of the Xinjiang Uygur Autonomous Region | Zhuang Y.,Peoples Hospital of the Xinjiang Uygur Autonomous Region
Experimental and Therapeutic Medicine | Year: 2016

The present study investigated the effects of transforming growth factor (TGF)-β3 on the regeneration of facial nerves in rabbits. A total of 20 adult rabbits were randomly divided into three equal groups: Normal control (n=10), surgical control (n=10) and TGF-β3 treatment (n=10). The total number and diameter of the regenerated nerve fibers was significantly increased in the TGF-β3 treatment group, as compared with in the surgical control group (P<0.01). Furthermore, in the TGF-β3 treatment group, the epineurial repair of the facial nerves was intact and the nerve fibers, which were arranged in neat rows, were morphologically intact with visible myelin swelling. However, in the surgical control group, the epineurial repair was incomplete, as demonstrated by: Atrophic nerve fibers, partially disappeared axons and myelin of uneven thickness with fuzzy borders. Electron microscopy demonstrated that the regenerated fibers in the TGF-β3 treatment group were predominantly myelinated, with clear-layered myelin sheath structures and axoplasms rich in organelles. Although typical layered myelin sheath structures were observed in the surgical control group, the myelin sheaths of the myelinated nerve fibers were poorly developed and few organelles were detected in the axoplasms. Neuro-electrophysiological examination demonstrated that, as compared with the surgical control group, the latency period of the action potentials in the TGF-β3 treatment group were shorter, whereas the stimulus amplitudes of the action potentials were significantly increased (P<0.01). The results of the present study suggest that TGF-β3 may improve the regeneration of facial nerves following trauma or injury. © 2016, Spandidos Publications. All Rights reserved. Source


Li J.,Hebei General Hospital | Wang X.,Hebei General Hospital | Zhang Y.,Hebei General Hospital | Zhang Y.,Gongli Hospital of Pudong New Area
Experimental and Therapeutic Medicine | Year: 2016

Cervical cancer is one of the most common gynecological cancers worldwide. Aberrant expression of E3 ubiquitin ligase isolated by differential display (EDD) has been detected in various types of tumor and has been demonstrated to have an important role in carcinogenesis, tumor growth and drug resistance. However, the role of EDD in cervical cancer and its underlying molecular mechanisms remains unknown. The present study aimed to investigate the role of EDD in the tumorigenicity of cervical cancer. EDD expression levels were measured using reverse transcription-quantitative polymerase chain reaction and western blotting in SiHa, HeLa, CaSki, c-41 and c-33A cervical cancer cell lines and cervical cancer tissue specimens. A functional study was performed using cell proliferation, colony formation, cell apoptosis assays in vitro and tumor growth assays in vivo with EDD either overexpressed or silenced. In the present study, EDD expression levels were significantly upregulated in cervical cancer cell lines and tissue samples. EDD knockdown significantly inhibited colony formation, cell proliferation and tumor growth and accelerated cell apoptosis in the cervical cancer cell lines and tissue samples. Furthermore, microRNA (miR)-143 expression levels were low in cervical cancer tissue samples and were negatively correlated with EDD expression. miR-143 silencing eliminated the effect of EDD on cell proliferation, colony formation and cell apoptosis in the cervical cancer cells, which suggested that miR-143 is critical for EDD-mediated regulation of cervical cancer cell growth. The results of the present study indicated that EDD may promote cervical cancer growth in vivo and in vitro by targeting miR-143. In conclusion, EDD may have an oncogenic role in cervical cancer and may serve as a potential therapeutic target for the treatment of patients with cervical cancer. © 2016, Spandidos Publications. All rights reserved. Source


Xia B.,Gongli Hospital of Pudong New Area | Wang X.,Gongli Hospital of Pudong New Area | Sun D.,Gongli Hospital of Pudong New Area
Chinese Journal of Rehabilitation Medicine | Year: 2011

Objective: To explore the feasibility of simple bladder capacity and pressure measurement in patients with neurogenic bladder after spinal cord injury(SCI). Method: Sixty SCI patients with neurogenic bladder were measured by simple bladder capacity and pressure measurement and urodynamic monitor for the bladder safe capacity measurement from October 2008 to May 2010 in Department of Rehabilitation and Orthopaedics. A comparison was made between the values obtained through the two lest means and the correlationship and test-retest reliability of simple bladder capacity and pressure measurement were analysed. Result: The bladder pressure values under different bladder capacities by the two test means had no statistically significant difference (P>0.05), and as suggested by the correlation analysis, r was 0.78-0.94 (P<0.05), It was shown that the two results were better correlated; and under different bladder capacities the intraclass correlation coefficients (ICC, model 2.1) of test-retest on simple bladder pressure measurement were all above 0.8. Conclusion: During lacking of bedside B - ultrasonicimage equipment, simple bladder capacity and pressure measurement is feasible as a replacement of urodynamic monitor testing in determining the bladder capacity of SCI patients. Source


Bi X.,Gongli Hospital of Pudong New Area | Wang X.,Gongli Hospital of Pudong New Area | Sun D.,Gongli Hospital of Pudong New Area
Chinese Journal of Rehabilitation Medicine | Year: 2011

Objective: To explore the effects of pelvic floor electrical stimulation (PES) on neurogenic bladder in patients with spinal cord injury(SCI). Method: Sixty patients with SCI were randomly divided into two groups:PES group and comentional therapy group, thirty patients in each group. Conventional therapy group was treated with intermittent catheterization, pro-vocative urination maneuver or Crede maneuver, Valsalva maneuver PES group was treated with PES combined with conventional therapy. The effects of two groups were assessed with urination frequency, average bladder ca-pacity.maximum urinary output,residual urine,quality of life, international scores of lower urinary tract symptom (LUTS) before and after treatment. Result: The urination frequency, the average bladder capacity,maximum urinary output.residual urine quality of life, and international scores of LUTS improved significantly in patients two months after lreatment(P<0.05),Curative effect of PES group was significantly better than that of conventional therapy group(P<0.05). Conclusion: Curative effect of PES combined with conventional therapy was significantly better than that of conventional therapy for the neurogenic bladder in patients with SCI. Source

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