Igumnov S.,Belarusian State University |
Lapanov P.,Gomel State Medical University
Psychiatry, Psychotherapy and Clinical Psychology | Year: 2016
The review analyzes clinical trials of the early and long-term mental disorders of the liquidators of the Chernobyl accident. Risk factors, pathogenesis and nosology forms of mental disorders, the basic principles of treatment and rehabilitation were consistently investigated. Radiation, psychological and other non-radiaton risk factors were scrutinized. Pathogenesis and nosological forms of psychoorganic, neurotic, psychosomatic and addictive disorders were considered. The basic principles of pharmacotherapy, psychotherapy and rehabilitation of liquidators were researched.
Starodubtseva M.N.,Gomel State Medical University |
Starodubtsev I.E.,Gomel State Medical University |
Starodubtsev E.G.,Gomel State Technical University
Micron | Year: 2017
Fractal dimension (DF) is one of the important parameters in the description of object's properties in different fields including biology and medicine. The present paper is focused on the application of the fractal dimension (the box counting dimension) in the analysis of the properties of cell surface on the base of its images obtained by atomic force microscopy (AFM). Fractal dimension of digital 3D AFM images depends on interpoint distances determined by the scanning step in the XY-plane and Z-scale factor t. We have studied the dependence of DF of AFM images on the Z-scale factor (DF = φ(t)) with purpose to reveal the features of the dependence and its usefulness in the analysis of the maps of surface properties. Using the model digital surfaces such as the plane, sinusoidal surfaces and “hilly” surface, we revealed that the sizes and spatial frequency of surface structural elements determined the basic features of the dependence (the parameters of peaks on the curve DF = φ(t)) and the element of chance in the localization of the structural elements on the surface had no significant influence on the dependence. Our findings demonstrate that the dependence of the fractal dimension on the Z-scale factor characterizes the structure of the AFM images more comprehensively than the roughness Ra and fractal dimension DF evaluated at a certain t. The dependence DF = φ(t) can be considered as a novel characteristic of AFM images. On analyzing the AFM images (lateral force maps) of glutaraldehyde-fixed adhered human fibroblasts and A549 human lung epithelial cells we found the significant difference in the dependences DF = φ(t) for different cell types that could be related to the difference of structural and mechanical surface properties of the studied cells. © 2017 Elsevier Ltd
Yarets Y.I.,Republican Scientific and Practical Center for Radiation Medicine and Human Ecology |
Rubanov L.N.,Republican Scientific and Practical Center for Radiation Medicine and Human Ecology |
Dundarov Z.A.,Gomel State Medical University
Novosti Khirurgii | Year: 2017
Objectives. To estimate the possibility of using the laboratory tests for evaluating the functional activity of the blood polymorphonuclear leukocytes (PNL) to predict skin grafting (SG) results in patients with chronic wounds (CW). Methods. The analysis of the functional activity of peripheral blood neutrophils was carried out in patients with chronic wounds (n=148) requiring skin grafting (SG). Ingestion capacity of neutrophils was determined in the phagocytosis reaction with the evaluation of phagocytic index (PhI) and phagocytic number (PhN). Oxygenproducing activity of neutrophils was estimated in response of the spontaneous NBT-test (NBTsp) and S. aureusstimulated (NBTst) nitroblue tetrazolium restoration. Activity of neutrophil extracellular traps (NET) in the blood were quantified prior (spontaneous; NETsp) and after neutrophils incubation with the stimulator (stimulated; NETst). Taking into account the differences of autodermoplasty outcomes within the incisional period all the patients were divided into 2 groups: group 1 (n=126) - favorable results of skin grafting and group 2 (n=22) - SG was complicated by lysis of autodermograft. Results. With the usage of logistic regression and ROC-curves methods the informativeness of NBTsp, NETsp, NETst for flap viability prognosis was established in patients with chronic wounds. The optimal cut-off value for NBTsp is 15% (86,5% sensitivity and 93% specificity), NETsp - 7% (97% sensitivity and 95,2% specificity), NETst - 15% (86,4% sensitivity and 90% specificity). At preoperative values of NBT, NETsp, NETst below cutoff, rejection of the autodermotransplant is considered to be predicted, and at NBT, NETsp, NETst above cut-off values, analysis demonstrates engraftment rates with a probability up to 92%. Conclusion. The values of functional activity of neutrophils (NBTsp, NETsp, NETst) are considered to be the informative predictors of the final outcome in patients with chronic wounds and they are also recommended for monitoring the efficiency of additional preoperative preparation in SG planning.
Hamaliaka A.,Gomel State Medical University |
Novikova I.,Gomel State Medical University
Biomedical Papers | Year: 2010
Aim. The prop ensity for certain individuals to develop staphylococcal recurrent furunculosis (RF) is not fully understood. But among the reasons of its development the immune system dysfunctions are described. As in the staphylococcus elimination the main role is played by neutrophils, the objective of this study was to determine nitric oxide (NO) and reactive oxygen species (ROS) production by polymo rphonuclear leukocytes (PMNs) of patients with RF and to compare them with the response of normal cells to stimulation. & Materials and methods. The spontaneous and pyrogenal-stimulated nitric oxide production was evaluated in leukocyte cell cultures by Griess reaction, and ROS generation was determined in the stimulated and unstimulated NBT-test. & Results. In this study we have demonstrated that leukocytes of healthy subjects respond on stimulation by the increase of both NO and ROS production. In contrast, leukocytes of patients with RF react by depression of NO formation at stimulation, and are characterized by decrease of ROS production reserve with the increasing of spontaneous ROS generation. Described disorders are revealed in the remission period as well as at exacerbation of furunculosis. & Conclusion. The present study suggests that leukocytes of patients with RF have stable defect of stimulated NO production increase, which can be the reason for recurrent and severe course of furunculosis.© A. Hamaliaka, I. Novikova.
Kazlouskaya V.,Gomel State Medical University |
Wittmann C.,New Hill |
Tsikhanouskaya I.,Vitebsk State Medical University
International Journal of Dermatology | Year: 2014
Background: Pustular syphilis is an extremely rare manifestation of secondary syphilis. Failure to recognize the disease can have devastating consequences. Methods: We present three patients with pustular syphilis as the primary manifestation of secondary syphilis. Results: Patient 1 was initially diagnosed by Venereal Disease Research Laboratory (VDRL) test (titers 1 : 32) and confirmed by enzyme immunoassay (EIA) for Treponema pallidum. Patient 2 was screened for syphilis by VDRL (titers 1 : 64), yielding a positive result, and tested negative for HIV. Secondary syphilis was confirmed by EIA. In Patient 3, a diagnosis of secondary syphilis was established by VDRL (titers 1 : 128) and EIA. Treponema pallidum was detected by dark field microscopy in three patients. Testing for HIV infection was negative in all patients. Conclusions: Diagnosis in secondary syphilis remains challenging because of the diversity of clinical presentations. The most commonly observed cutaneous presentation is a generalized, non-pruritic, papulosquamous eruption varying from pink to violaceous to brown, with mucous membrane involvement. The diagnostic methods used to identify secondary syphilis are the same as those used to diagnose other stages of syphilitic infection. The persistence of syphilis in both developed and underdeveloped regions highlights the importance of considering syphilitic infection in the setting of a cutaneous pustular eruption, especially one that fails to respond to standard therapy. Testing for other sexually transmitted diseases, including HIV, should be performed in all patients diagnosed with syphilis. © 2014 The International Society of Dermatology.
Edelstein M.V.,Smolensk State Medical Academy |
Skleenova E.N.,Smolensk State Medical Academy |
Shevchenko O.V.,Smolensk State Medical Academy |
D'souza J.W.,Smolensk State Medical Academy |
And 7 more authors.
The Lancet Infectious Diseases | Year: 2013
Background: Multidrug-resistant and extensively-drug-resistant Pseudomonas aeruginosa are increasing therapeutic challenges worldwide. We did a longitudinal epidemiological and clinical study of extensively-drug-resistant P aeruginosa in Belarus, Kazakhstan, and Russia. Methods: The study was done in three prospectively defined phases: Jan 1, 2002-Dec 31, 2004; Jan 1, 2006-Dec 31, 2007; and Jan 1, 2008-Dec 31, 2010. The first two phases were in Russia only. All consecutive, non-duplicate, nosocomial isolates and case report forms were sent to the coordinating centre (Institute of Antimicrobial Chemotherapy, Smolensk, Russia), where species reidentification, susceptibility testing, and molecular typing of isolates were done. We did susceptibility testing by agar dilution. The presence of metallo-β-lactamase (MBL) genes was established by PCR and sequencing, and class 1 integrons containing MBL gene cassettes were analysed by the PCR restriction fragment length polymorphism approach. Strain relatedness was analysed by multiple loci variable-number tandem-repeat (VNTR) analysis (at six VNTR loci) and multilocus sequence typing. Results: In 2002-04, 628 of 1053 P aeruginosa isolates were insusceptible to carbapenems and 47 (4·5%) possessed MBLs. In 2006-07, 584 of 787 isolates were insusceptible to carbapenems and 160 (20·3%) possessed MBLs. In 2008-10, 1238 of 1643 Russian P aeruginosa isolates were insusceptible to carbapenems and 471 (28·7%) possessed MBLs. Additionally, the 32 P aeruginosa isolates from Belarus and Kazakhstan were all carbapenem insusceptible and all possessed MBLs. More than 96% of MBL-positive P aeruginosa isolates were resistant to all antibiotics except colistin (ie, extensively drug resistant), and, in 2010, 5·9% were resistant to colistin. 685 (96·5%) of 710 MBL-positive P aeruginosa belonged to ST235. blaVIM-2 genes were detected in 707 (99·6%) of 710 MBL-positive isolates. Interpretation: Extensively-drug-resistant ST235 P aeruginosa has rapidly spread throughout Russia and into Belarus and Kazakhstan via clonal dissemination. Increases in the use of colistin will probably result in further spread of ST235 P aeruginosa resistant to all drugs. Funding: HEFC, Ministry of Health of the Russian Federation, Government of the Republic of Belarus, Government of the Republic of Kazakhstan, European Union, Medical Research Council UK-Canada partnership. © 2013 Elsevier Ltd.
Sergeenko M.N.,Gomel State Medical University
Nonlinear Phenomena in Complex Systems | Year: 2014
Complex masses of resonances in the quark potential model are obtained. Two exact asymptotic solutions for the QCD motivated potential are used to derive the resonance complex-mass formula. The centered masses and total widths of mesonic resonances are calculated. A possible origin of the dark matter is discussed. © 2014 Education and Upbringing Publishing. All Rights reserved.
Starodubtseva M.N.,Gomel State Medical University
Ageing Research Reviews | Year: 2011
Mechanical properties are fundamental properties of the cells and tissues of living organisms. The mechanical properties of a single cell as a biocomposite are determined by the interdependent combination of cellular components mechanical properties. Quantitative estimate of the cell mechanical properties depends on a cell state, method of measurement, and used theoretical model. Predominant tendency for the majority of cells with ageing is an increase of cell stiffness and a decrease of cell ability to undergo reversible large deformations. The mechanical signal transduction in old cells becomes less effective than that in young cells, and with ageing, the cells lose the ability of the rapid functional rearrangements of cellular skeleton. The article reviews the theoretical and experimental facts touching the age-related changes of the mechanical properties of cellular components and cells in the certain systems of an organism (the blood, the vascular system, the musculoskeletal system, the lens, and the epithelium). In fact, the cell mechanical parameters (including elastic modulii) can be useful as specific markers of cell ageing. © 2009 Elsevier Ireland Ltd.
Novikova I.,Gomel State Medical University |
Zlotnikova M.,Gomel State Medical University
Biomedical Papers | Year: 2011
Background. Research interest in ceruloplasmin (CP) has significantly increased in recent years owing to new discoveries of its properties including anti-inflammatory and anti-oxidant effects. Data on CP blood plasma level in patients with herpetic infection caused by herpes simplex virus are scarce and often contradictory. Most point to a reduction of CP in the blood plasma of patients during the exacerbation of the disease with gradually return to normal values after treatment. There is evidence of an increase in CP levels during the acute period of CRHI (chronic recurrent herpes infection) and decrease in remission. Materials and methods. The content of ceruloplasmin in blood plasma was determined by immunoturbidimetry using the test-systems "Spinreact" (Spain) and biochemical analyzer "Architect C8000". Results. We found that in patients with severe forms of chronic recurrent herpes infection in the exacerbation period, CP levels were increased by approximately 35% relative to the control values. However in the remission period in 80% of patients, CP concentrations remained elevated and in some patients the CP level was even increased in comparison with values in the exacerbation. Such dynamics of CP were not caused by the exacerbation of concomitant diseases. Conclusion. The increased CP levels in the remission period in patients with severe forms of chronic recurrent herpes infection may have been caused by its effect as an endogenous antioxidant. No correlations of CP levels with other laboratory signs of acute inflammation were found but identified was a relation to free radical activity. © I. Novikova, M. Zlotnikova.
Novikova I.,Gomel State Medical University
Biomedical papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia | Year: 2011
Research interest in ceruloplasmin (CP) has significantly increased in recent years owing to new discoveries of its properties including anti-inflammatory and anti-oxidant effects. Data on CP blood plasma level in patients with herpetic infection caused by herpes simplex virus are scarce and often contradictory. Most point to a reduction of CP in the blood plasma of patients during the exacerbation of the disease with gradually return to normal values after treatment. There is evidence of an increase in CP levels during the acute period of CRHI (chronic recurrent herpes infection) and decrease in remission. The content of ceruloplasmin in blood plasma was determined by immunoturbidimetry using the test-systems "Spinreact" (Spain) and biochemical analyzer "Architect C8000". We found that in patients with severe forms of chronic recurrent herpes infection in the exacerbation period, CP levels were increased by approximately 35% relative to the control values. However in the remission period in 80% of patients, CP concentrations remained elevated and in some patients the CP level was even increased in comparison with values in the exacerbation. Such dynamics of CP were not caused by the exacerbation of concomitant diseases. The increased CP levels in the remission period in patients with severe forms of chronic recurrent herpes infection may have been caused by its effect as an endogenous antioxidant. No correlations of CP levels with other laboratory signs of acute inflammation were found but identified was a relation to free radical activity.