Goesan Agricultural Technology and Extension Center

Cheong ju, South Korea

Goesan Agricultural Technology and Extension Center

Cheong ju, South Korea

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Kamal A.H.M.,Chungbuk National University | Jang I.-D.,Chungbuk National University | Kim D.-E.,Chungbuk National University | Suzuki T.,Japan National Agriculture and Food Research Organization | And 9 more authors.
Journal of Plant Biology | Year: 2011

We used proteomics analysis to generate the profiles of proteins in the endosperm and embryo of common buckwheat grains. These differentially expressed proteins are potentially involved in seed metabolism. Extractions were done by trichloroacetic acid (TCA) precipitation. The resulting proteins were separated using SDS-PAGE coupled to LC-ESI-Q/TOF-MS/MS. This allowed us to detect and identify 67 proteins with isoforms, making this the most inclusive protein profile. The proteins were determined to be functionally involved in the central metabolic pathway of the seed, with metabolic interest being reflected in the occurrence of a tissue-specific enzyme balance. For a case in point, we found a tissue-specific and subcellular compartment-specific isoform of granule-bound starch synthase 1 in the chloroplast/amyloplast. This provided proteomic verification of the presence of a distinct regulatory mechanism for the biosynthesis of glycan and starch, which produce amylase and amylopectin. Furthermore, several previously characterized allergenic proteins such as 11S and 13S globulin seed storage protein were acknowledged in our seed samples, thus representing the potential for proteomics techniques that survey food sources for any incidence of allergens. This protein profile of common buckwheat grain is a new avenue for understanding its seed physiology in dormant stage as well as suggesting commercial applications for the buckwheat industry as buckwheat flour. © 2011 The Botanical Society of Korea.


Lee S.H.,Chungbuk National University | Hwang I.G.,Chungbuk National University | Kim H.Y.,Chungbuk National University | Lee H.K.,Chungbuk National University | And 4 more authors.
Journal of the Korean Society of Food Science and Nutrition | Year: 2010

This study investigated starch properties of 'Daehak waxy corn (DWC)' with different harvest times. The DWCs were harvested at 4 days before suitable time (BST), suitable time (ST) and 4 days after suitable time (AST). As harvest time was delayed, starch yield and amylopectin content of DWC starch increased from 43.21 to 52.73%, and from 90.79 to 92.83% based on dry weight, respectively. As harvest time was postponed, enzymatic digestibility and water solubility of DWC starch decreased from 81.43 to 80.58%, and from 10.23 to 9.23%. However, water binding capacity and swelling power of DWC starch increased from 227.94 to 244.88%, and from 24.75 to 29.74%, respectively. Retrogradation viscosity of starch was the lowest in DWC harvested at AST. There was a high correlation coefficient among starch properties of DWC, such as starch yield, enzymatic digestibility, water binding capacity, swelling power, water solubility and retrogradation viscosity (p<0.01). As harvest time was deferred, enzymatic digestibility, water solubility and retrogradation viscosity decreased; however, amylopectin content increased, and water binding capacity and swelling power significantly increased with increasing amylopectin content.


Lee S.H.,Chungbuk National University | Hwang I.G.,Chungbuk National University | Kim H.Y.,Chungbuk National University | Lee H.K.,Chungbuk National University | And 4 more authors.
Journal of the Korean Society of Food Science and Nutrition | Year: 2010

This study investigated the physicochemical characteristics and antioxidant activities of Daehak waxy corns harvested at different times. Daehak waxy corns were harvested at 4 days before suitable time (BST), suitable time (ST) and 4 days after suitable time (AST). As harvest time was delayed, weight, length and width of kernel, the crude ash, lipid and protein contents significantly increased. Fructose, glucose and sucrose contents of BST were higher than those of ST and AST. Copper, sodium, calcium and potassium contents of BST in Daehak waxy corns increased on different times, and contents of ferrous and zinc did not show any significantly difference. The major fatty acids were oleic acid (48.58~49.96%), palmitic acid (19.50~20.64%) and palmitoleic acid (18.70~23.77%) in Daehak waxy corns with different harvest times. The highest total polyphenol content and total antioxidant capacity of EtOH extracts from AST were 104.45 mg/100 g and 20.53 mg AA eq/100 g, respectively. There was a high correlation between total polyphenol content and IC50 value of electron donating ability of EtOH extracts from Daehak waxy corns harvested at different times, or total antioxidant capacity (p<0.01).

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