Poitevin Y.,NovImmune |
Pontini G.,NovImmune |
Fischer N.,NovImmune |
Kosco-Vilbois M.,NovImmune |
Elson G.,Glenmark Pharmaceuticals SA
Journal of Immunological Methods | Year: 2017
To establish a simple and widely accessible technique for rapidly selecting high producing Chinese hamster ovary (CHO) cells engineered to express a monoclonal antibody (mAb), we have exploited the transient display of recombinant protein on their cell surface. In combination with magnetic bead-based methods, we demonstrate the ability to select for cells of high productivity in the absence of any metabolic-based selection method. This technique is sufficient to obtain genetically stable engineered CHO cells via a single step of cell subcloning and yields sought-after stable, high IgG producing clonal cell lines. This technique may also be applied to other types of cells as well as polyclonal Ab cell pools. © 2017 Elsevier B.V.