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Deb P.,Girijananda Chowdhury Institute of Pharmaceutical Science Guwahati | Dash S.,Girijananda Chowdhury Institute of Pharmaceutical Science Guwahati | Murthy P.N.,Girijananda Chowdhury Institute of Pharmaceutical Science Guwahati
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2014

Objective: The present study was undertaken with an objective to expound the physicochemical, and mucoadhesive properties of polysaccharide obtained from Hibiscus esculentus Linn (HEP). The extraction of polysaccharide was maximized employing design of experiment by using response surface methodology. Methods: The crude polysaccharide was purified by gel filtration chromatographic method. Elemental analysis, Scanning electron microscopy (SEM), X-ray diffraction crystallography (XRD), Differential scanning calorimetry (DSC), Fourier-transformed infrared spectroscopy (FT-IR) and Nuclear Magnetic Resonance (NMR) spectroscopy techniques were employed to characterize the polysaccharide. The mucoadhesive property of the polysaccharide was determined by Wilhelmy plate and falling liquid film technique. Results: Optimization of process variables leads to an experimental yield of 8.32% w/w with relative deviation of 0.56% between the experimental and the predicted values. Elemental analysis of the polysaccharide has shown the contents of carbon, hydrogen and nitrogen to be 29.5%, 5.67% and 1.7% (w/w) respectively. SEM micrograph analysis suggests that the polysaccharide has irregular particle size and mostly seen in aggregates. The XRD pattern of the polysaccharide indicates both crystalline and amorphous structure. The DSC and FT-IR data also confirmed the presence of polysaccharide structure. The ex-vivo mucoadhesive study by Wilhelmy plate Method showed detachment force of 1.7gm, 2.3gm and 3.1gm for 1%, 2% and 3% w/w HEP gel respectively. Conclusion: The experimental work provides enough evidence to exploit this natural biopolymer in pharmaceutical food and cosmetic industry. Ex-vivo mucoadhesion study demonstrated the usefulness of HEP as a bioadhesive agent for pharmaceutical dosage forms.

PubMed | Hong Kong Baptist University, Hayat Hospital Guwahati, Jawaharlal Nehru Centre for Advanced Scientific Research, Girijananda Chowdhury Institute of Pharmaceutical Science Guwahati and Institute of Advanced Study in Science and Technology Guwahati
Type: | Journal: Frontiers in pharmacology | Year: 2016

The tribal communities of North Eastern India rely on herbal medicine to cure various disease conditions. Ziziphus jujuba Mill. (Rhamnaceae) is one of such medicinal plants used for curing liver ailments, insomnia, anemia, diarrhea, diabetic complications, cancer, and loss of appetite. The present study was aimed to describe the protective ability of Z. jujuba root bark (ZJRB) against hepatic injury and chronic inflammation. Bioactivity guided fractionation of Z. jujuba methanol extract (ZJME) was performed using different solvents of increasing polarity viz. hexane (ZJHF), chloroform (ZJCF), ethyl acetate (ZJEAF), water (ZJWF), and residue (ZJMR). In vitro antioxidant results revealed that both ZJME and ZJWF possess strong antioxidant activity among all the fractions and mother extract tested. Further, ZJME and ZJWF showed significant protection against CCl4 intoxicated HepG2 cell lines by means of increased cell viability and decreased LDH levels compared to control group. ZJME at 200, 400 mg/kg and ZJWF at 50, 100 mg/kg inhibited the lipid peroxidation and significantly restored the liver function markers (AST, ALT, ALP, LDH, SOD, and CAT) and cytokine levels (TNF-, Il-1, and Il-10) in CCl4 induced acute liver damage in rats. All the results were comparable with standard drug silymarin which was further confirmed by histopathology analysis of liver. Similarly, inflammation and increase inflammatory cytokines levels of carrageenan induced paw edema in rats have been refurbished to normal levels on par with the standard drug indomethacin. ZJWF demonstrated potent response than ZJME in all the biological tests conducted. The results of the study signify the ability of ZJRB as good therapeutic agent for liver toxicity and chronic inflammation.

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