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Middleton, WI, United States

Reinitz C.A.,University of Wisconsin - Madison | Pleva A.E.,University of Wisconsin - Madison | Pleva A.E.,Gilson Inc. | Stretton A.O.W.,University of Wisconsin - Madison
Molecular and Biochemical Parasitology | Year: 2011

Recent technical advances have rapidly advanced the discovery of novel peptides, as well as the transcripts that encode them, in the parasitic nematode Ascaris suum. Here we report that many of these novel peptides produce profound and varied effects on locomotory behavior and levels of cyclic nucleotides in A. suum. We investigated the effects of 31 endogenous neuropeptides encoded by transcripts afp-1, afp-2, afp-4, afp-6, afp-7, and afp-9-14 (afp: Ascaris FMRFamide-like Precursor protein) on cyclic nucleotide levels, body length and locomotory behavior. Worms were induced to generate anteriorly propagating waveforms, peptides were injected into the pseudocoelomic cavity, and changes in the specific activity (nmol/mg protein) of second messengers cAMP (3′5′ cyclic adenosine monophosphate) and cGMP (3′5′ cyclic guanosine monophosphate) were determined. Many of these neuropeptides changed the levels of cAMP (both increases and decreases were found), whereas few neuropeptides changed the level of cGMP. A subset of the peptides that lowered cAMP was investigated for effects on the locomotory waveform and on body length. Injection of AF19, or AF34 (afp-13), AF9 (afp-14), AF26 or AF41 (afp-11) caused immediate paralysis and cessation of propagating body waveforms. These neuropeptides also significantly increased body length. In contrast, injection of AF15 (afp-9) reduced the body length, and decreased the amplitude of waves in the body waveform. AF30 (afp-10) produced worms with tight ventral coils. Although injection of neuropeptides encoded by afp-1 (AF3, AF4, AF10 or AF13) produced an increased number of exaggerated body waves, there were no effects on either cAMP or cGMP. By injecting peptides into behaving A. suum, we have provided an initial screen of the effects of novel peptides on several behavioral and biochemical parameters. © 2011 Elsevier B.V. All rights reserved. Source


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Trademark
Gilson Inc. | Date: 2013-08-05

laboratory apparatus, namely, instruments for solid phase extraction of compounds.; laboratory consumables, namely, solid phase extraction cartridges, solid phase extraction plates, and solid phase extraction columns.


Patent
Gilson Inc. | Date: 2013-10-03

A method of creating a protocol is provided. An interface window is presented on a display. A first indicator that identifies a sample and a second indicator that indicates selection of an analysis are received from the interface window. The analysis defines processing to be performed on the identified sample by a liquid handler. A bed layout that defines locations of a plurality of labware components and a type of labware component at each location on a work bed of the liquid handler is determined based on the first indicator and the second indicator. The sample is associated with a location of the locations. The determined bed layout is presented on the display. A protocol for execution by a controller of the liquid handler is created. The protocol comprises a second plurality of instructions configured to cause the liquid handler to perform the analysis on the identified sample.


Patent
Gilson Inc. | Date: 2013-03-14

A translational support system is provided that includes a first wall and a second wall, a first support bracket mounted to the first wall, a second support bracket mounted to the second wall, a bearing rod, a base, a bearing carriage, and a rigid support. The bearing rod is mounted between the brackets. The base is mounted between the walls and includes a base plate having a first side and a second side and a slot formed through the base plate. The bearing carriage is positioned on the first side of the base plate and mounted to translate along the bearing rod in a plane parallel to the first side. The bearing carriage includes a leg mounted to a device support structure through the slot. The rigid support is mounted to the second side of the base plate. The rigid support supports the device support structure above the base plate.

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