Nagy J.,MetroHealth Rehabilitation Institute of Ohio |
Winslow A.,MetroHealth Rehabilitation Institute of Ohio |
Brown J.,MetroHealth Rehabilitation Institute of Ohio |
Brown J.,University of Kansas |
And 7 more authors.
Topics in Spinal Cord Injury Rehabilitation | Year: 2012
Objective: To assess the peak force during wheelchair propulsion of individuals with spinal cord injury propelling over obstacles from the Wheelchair Skills Test. Participants/Methods: Twenty-three individuals with spinal cord injury (SCI) who are full-time manual wheelchair users were included in this prospective study. A SmartWheel (Three Rivers Holdings, LLC) was used to analyze each push while subjects negotiated standardized obstacles used in the Wheelchair Skills Test, including tile, carpet, soft surface, 5°and 10°ramps, 2 cm, 5 cm, and 15 cm curbs. Results: When the peak forces of the advanced skills were compared to level 10 m tile/10 m carpet, there was a statistically significant increase in all peak forces (P value ranged from .0001 to .0268). Discussion: It is well documented that a large number of individuals with SCI develop upper limb pain. One of the recommendations to preserve the upper limb is to minimize force during repetitive tasks. Conclusion: Advanced wheelchair skills require an increase in force to accomplish. The increase in forces ranged from 18% to 130% over that required for level 10 m tile/10 m carnet. © 2012 Thomas Land Publishers, Inc.
Gilmour Academy | Date: 2011-10-18
clothing, namely, shirts, jackets, and caps. Educational services, namely, providing courses of instruction at the primary, secondary, and pre-school levels, and distributing course materials therewith; entertainment services in the nature of arranging and conducting athletic competitions, live performances by musical bands, live performances by musical choral groups, presentation of dramatic performances in the nature of plays, and art exhibitions.
Gilmour Academy | Date: 2012-02-28
Turk E.M.,Case Western Reserve University |
Turk E.M.,Gilmour Academy |
Das V.,Case Western Reserve University |
Seibert R.D.,Gilmour Academy |
Andrulis E.D.,Case Western Reserve University
PLoS ONE | Year: 2013
Mitochondria are essential organelles that harbor a reduced genome, and expression of that genome requires regulated metabolism of its transcriptome by nuclear-encoded proteins. Despite extensive investigation, a comprehensive map of the yeast mitochondrial transcriptome has not been developed and all of the RNA-metabolizing proteins have not been identified, both of which are prerequisites to elucidating the basic RNA biology of mitochondria. Here, we present a mitochondrial transcriptome map of the yeast S288C reference strain. Using RNAseq and bioinformatics, we show the expression level of all transcripts, revise all promoter, origin of replication, and tRNA annotations, and demonstrate for the first time the existence of alternative splicing, mirror RNAs, and a novel RNA processing site in yeast mitochondria. The transcriptome map has revealed new aspects of mitochondrial RNA biology and we expect it will serve as a valuable resource. As a complement to the map, we present our compilation of all known yeast nuclear-encoded ribonucleases (RNases), and a screen of this dataset for those that are imported into mitochondria. We sought to identify RNases that are refractory to recovery in traditional mitochondrial screens due to an essential function or eclipsed accumulation in another cellular compartment. Using this in silico approach, the essential RNase of the nuclear and cytoplasmic exosome, Dis3p, emerges as a strong candidate. Bioinformatics and in vivo analyses show that Dis3p has a conserved and functional mitochondrial-targeting signal (MTS). A clean and marker-less chromosomal deletion of the Dis3p MTS results in a defect in the decay of intron and mirror RNAs, thus revealing a role for Dis3p in mitochondrial RNA decay. © 2013 Turk et al.
Mansour J.M.,Case Western Reserve University |
Gu D.-W.M.,Case Western Reserve University |
Chung C.-Y.,Case Western Reserve University |
Heebner J.,Case Western Reserve University |
And 5 more authors.
Annals of Biomedical Engineering | Year: 2014
Our ultimate goal is to non-destructively evaluate mechanical properties of tissue-engineered (TE) cartilage using ultrasound (US). We used agarose gels as surrogates for TE cartilage. Previously, we showed that mechanical properties measured using conventional methods were related to those measured using US, which suggested a way to non-destructively predict mechanical properties of samples with known volume fractions. In this study, we sought to determine whether the mechanical properties of samples, with unknown volume fractions could be predicted by US. Aggregate moduli were calculated for hydrogels as a function of SOS, based on concentration and density using a poroelastic model. The data were used to train a statistical model, which we then used to predict volume fractions and mechanical properties of unknown samples. Young’s and storage moduli were measured mechanically. The statistical model generally predicted the Young’s moduli in compression to within <10% of their mechanically measured value. We defined positive linear correlations between the aggregate modulus predicted from US and both the storage and Young’s moduli determined from mechanical tests. Mechanical properties of hydrogels with unknown volume fractions can be predicted successfully from US measurements. This method has the potential to predict mechanical properties of TE cartilage non-destructively in a bioreactor. © 2014, Biomedical Engineering Society.