Hiroshige Y.,Nagoya University |
Hara M.,Saitama University |
Nagai A.,Gifu UniversityGifu |
Hikitsuchi T.,Dainihon Jochugiku Co. |
And 7 more authors.
PLoS ONE | Year: 2017
Mosquitoes occur almost worldwide, and females of some species feed on blood from humans and other animals to support ovum maturation. In warm and hot seasons, such as the summer in Japan, fed mosquitoes are often observed at crime scenes. The current study attempted to estimate the time that elapsed since feeding from the degree of human DNA digestion in mosquito blood meals and also to identify the individual human sources of the DNA using genotyping in two species of mosquito: Culex pipiens pallens and Aedes albopictus. After stereomicroscopic observation, the extracted DNA samples were quantified using a human DNA quantification and quality control kit and were genotyped for 15 short tandem repeats using a commercial multiplexing kit. It took about 3 days for the complete digestion of a blood meal, and genotyping was possible until 2 days post-feeding. The relative peak heights of the 15 STRs and DNA concentrations were useful for estimating the post-feeding time to approximately half a day between 0 and 2 days. Furthermore, the quantitative ratios derived from STR peak heights and the quality control kit (Q129/Q41, Q305/Q41, and Q305/Q129) were reasonably effective for estimating the approximate post-feeding time after 2–3 days. We suggest that this study may be very useful for estimating the time since a mosquito fed from blood meal DNA, although further refinements are necessary to estimate the times more accurately. © 2017 Hiroshige et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Tanikawa K.,Yokohama National University |
Ohno K.,Yokohama National University |
Noda Y.,Japan National Institute of Materials Science |
Ono S.,Gifu UniversityGifu |
And 4 more authors.
Chemical Physics Letters | Year: 2017
We have performed first-principles density functional calculations of a molecular heterojunction of a zinc phthalocyanine (ZnPc) molecule and a peanut-shaped fullerene polymer (PSFP) made from several coalesced cross-linked C60 molecules. The PSFP has many isomers and all have both spatially localized (near ZnPc) and metallic conducting levels. Here we consider four typical isomers. From the resulting electronic structure, we discuss the applicability of these isomers to organic photovoltaics (OPV), electrodes, and light harvesting materials. If one of the isomers called T3, which has the largest energy gap, is used together with ZnPc for OPV, this system shows more than 20% energy conversion efficiency. © 2017
Pal B.,University of Cincinnati |
Schwartz A.J.,University of Cincinnati |
Abdesselam A.,University of Tabuk |
Adachi I.,Graduate University for Advanced Studies |
And 187 more authors.
Physical Review D - Particles, Fields, Gravitation and Cosmology | Year: 2015
We report a search for the charmless hadronic decay B0→ηπ0 with a data sample corresponding to an integrated luminosity of 694 fb-1 containing 753×106 BB¯ pairs. The data were collected by the Belle experiment running on the Y(4S) resonance at the KEKB e+e- collider. We measure a branching fraction B(B0→ηπ0)=(4.1-1.5-0.7+1.7+0.5)×10-7, where the first uncertainty is statistical and the second is systematic. Our measurement gives an upper limit of B(B0→ηπ0)<6.5×10-7 at 90% confidence level. The signal has a significance of 3.0 standard deviations and constitutes the first evidence for this decay mode. © 2015 American Physical Society. © 2015 American Physical Society.
Tada M.,Niigata UniversityNiigata |
Konno T.,Niigata UniversityNiigata |
Tezuka T.,Niigata UniversityNiigata |
Miura T.,Niigata UniversityNiigata |
And 17 more authors.
Annals of Neurology | Year: 2016
Objective: To clarify the histopathological alterations of microglia in the brains of patients with hereditary diffuse leukoencephalopathy with spheroids (HDLS) caused by mutations of the gene encoding the colony stimulating factor-1 receptor (CSF-1R). Methods: We examined 5 autopsied brains and 1 biopsy specimen from a total of 6 patients with CSF-1R mutations. Detailed immunohistochemical, biochemical, and ultrastructural features of microglia were examined, and quantitative analyses were performed. Results: In layers 3 to 4 of the frontal cortex in HDLS brains, microglia showed relatively uniform and delicate morphology, with thin and winding processes accompanying knotlike structures, and significantly smaller areas of Iba1 immunoreactivity and lower numbers of Iba1-positive cells were evident in comparison with control brains. On the other hand, in layers 5 to 6 and the underlying white matter, microglia were distributed unevenly; that is, in some areas they had accumulated densely, whereas in others they were scattered. Immunoblot analyses of microglia-associated proteins, including CD11b and DAP12, revealed that HDLS brains had significantly lower amounts of these proteins than diseased controls, although Ki-67–positive proliferative microglia were not reduced. Ultrastructurally, the microglial cytoplasm and processes in HDLS showed vesiculation of the rough endoplasmic reticulum and disaggregated polyribosomes, indicating depression of protein synthesis. On the other hand, macrophages were immunonegative for GLUT-5 or P2ry12, indicating that they were derived from bone marrow. Interpretation: The pathogenesis of HDLS seems to be associated with microglial vulnerability and morphological alterations. Ann Neurol 2016;80:554–565. © 2016 American Neurological Association
PubMed | Yangtze University, Gifu UniversityGifu and Yangtze UniversityJingzhou
Type: | Journal: Frontiers in plant science | Year: 2017
Predicted climate changes, in particular, the increased dimension and frequency of heat waves, are expected to affect crop growth in the future seriously. Hybrid rice relies on seed production from male sterile and restorer lines. Experiments were conducted over two consecutive years to compare the high temperature tolerance of parents of different hybrid rice combinations, in terms of fertility rate, flowering pattern, pollination and physiological parameters of the lodicule. Three male sterile lines and a broad compatibility restorer line (as pollen donor and conventional variety as well) were grown to heading stage and then treated with average daily temperatures of 26C (range 23-30C), 28C (25-32C), and 30C (26-34C), respectively, continued for 5-7 days each in a natural light phytotron which simulated the local typical high temperature weather in the field. The results indicated that male sterile lines were more sensitive to high temperature than the restorer line for fertility rate, and the sensitivity varied between varieties. The fertility rate of the restorer line was maintained at about 90% under the high temperature treatments, while it decreased in the male sterile lines by 23.3 and 48.1% at 28 and 30C, respectively. The fertility rate of the most sensitive line declined by 70%, and the tolerant line declined by 34% at 30C. Glume closure in the male sterile lines was a major reason for the reduced fertility rate under high temperature, which is closely correlated with carbohydrates content and the vascular bundle pattern in the lodicule. The present study identified a useful trait to select male sterile lines with high temperature tolerance for seed production.
Itaya Y.,Gifu UniversityGifu |
Kobayashi N.,Gifu UniversityGifu |
Li L.,Gifu UniversityGifu |
Suami A.,Gifu UniversityGifu |
And 2 more authors.
Drying Technology | Year: 2015
An innovative technology for sewage treatment systems is proposed. A mixture of sewage sludge and char particle is subjected to drying in the progress of composting in an updraft column. Exothermic heat generated during composting is utilized for energy of sludge drying. The char particle in the mixture contributes to enhancement of composting and drying rate. This study presents drying and composting behaviors of sludge to examine the effect of char addition. A fundamental drying experiment was carried out by hot-air heating of the sample in a small vessel. The drying characteristic curve for a mixture of sludge and char was greater than the sample of only sludge over a whole period if the drying rates were compared with the same moisture content. The drying behavior could be analyzed with a reasonable agreement by the water front receding model. The enhancement effect of drying and composting of sludge by char addition was confirmed by examination using a pilot scale of the updraft column whose volume was 50 m3. Sludge with 400% dry base (80% wet base) in moisture content could be dried successfully to lower moisture content than 67% dry base (40% wet base) only by exothermic heat during composting without supplying any auxiliary energy from the outside, and the treatment time was reduced by mixing char in sludge. The behavior was analyzed with a satisfactory agreement by a theoretical model employing moisture diffusion parameters determined from the drying characteristic curve. © 2015, Copyright © Taylor & Francis Group, LLC.
Sarker U.,Bangabandhu Sheikh Mujibur Rahman Agricultural University |
Islam M.T.,Bangabandhu Sheikh Mujibur Rahman Agricultural University |
Rabbani M.G.,Bangladesh Agricultural University |
Oba S.,Gifu UniversityGifu
Journal of Food, Agriculture and Environment | Year: 2014
This study aimed to evaluate genotypic variability in 30 vegetable amaranth genotypes for nutrient composition, antioxidant content, and 12 yield contributing traits. The trials were in the field of Bangabandhu Sheikh Mujibur Rahman Agricultural University in Bangladesh for consecutive two years (2012 and 2013) implementing Randomized Complete Block Design (RCBD) with three replications. High mean value, high range of variability and high genotypic variance were observed for all the traits except content of Ca, protein and betacarotenoid. Close differences between genotypic and phenotypic variances and genotypic and phenotypic coefficient of variations were observed for all the traits. Considering all genetic parameters, selection based on contents of potassium, manganese, and ascorbic acid, plant height, leaves/plant, diameter of stem base, fiber content, leaf area and foliage yield/plot seemed to be effective for the improvement of vegetable amaranth. Foliage yield had significant positive correlation with plant height, leaves per plant, diameter of stem base, fiber content and leaf area. Nutrient content and antioxidant traits exhibited interesting results, i.e., had insignificant genotypic correlations with foliage yield and most of the studied traits indicating that selection with these traits might be possible without compromising any yield loss. Therefore, concomitant selection for high nutrient, antioxidant and high foliage yield would be effective for improvement of the vegetable amaranth. Based on mean, range, genetic parameters, correlation coefficient and path coefficient values, direct selection through three traits, i.e., fiber content, leaf area and diameter of stem base would significantly improve the foliage yield of vegetable amaranth. On the other hand, concomitant selection based on high nutrient and antioxidant content and high foliage yield would be effective selection method for improvement of vegetable amaranth. © 2014, World Food Ltd. and WFL Publishers. All rights reserved.
PubMed | Gifu UniversityGifu, Gifu University, Iwate University and Awaji Agricultural Technology Center Minami Awaji
Type: | Journal: Frontiers in microbiology | Year: 2016
We investigated the relationship between ruminal pH and bacteria in calves fed calf starter with and without forage during weaning transition. First, 16 Holstein bull calves were obtained from dairy farms and equipped with rumen cannulas by cannulation surgery. Then, calves (73.5 4.2 kg; mean SE) were assigned to groups fed calf starter either with forage (HAY,