Gifu Prefectural Government

Gifu, Japan

Gifu Prefectural Government

Gifu, Japan
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Gato N.,Nakano BC Co. | Kadowaki A.,Nakano BC Co. | Hashimoto N.,Nakano BC Co. | Yokoyama S.-I.,Gifu Prefectural Government | Matsumoto K.,Ishikawa Prefectural University
Annals of Nutrition and Metabolism | Year: 2013

Bile acid-binding agents are known to lower blood cholesterol levels and have been clinically used for the treatment of hypercholesterolemia. We previously showed that tannin-rich fiber from young persimmon (Diospyros kaki) fruits had bile acid-binding properties. In this study, we performed a randomized, double-blind, placebo-controlled trial to investigate the hypocholesterolemic effects of tannin-rich fiber in humans. The subjects (n = 40, plasma total cholesterol levels 180-259 mg/dl) were divided into 3 groups and ingested cookie bars containing 0 g (placebo group, n = 14), 3 g (low-dose group, n = 13), or 5 g (high-dose group, n = 13) of tannin-rich fiber 3 times daily before meals for 12 weeks. Plasma total cholesterol levels decreased significantly in the low-dose (12 weeks, p < 0.005) and high-dose (6 weeks, p < 0.05; 12 weeks, p < 0.001) groups. In addition, plasma low-density lipoprotein cholesterol levels decreased significantly in the high-dose group (6 weeks, p < 0.05; 12 weeks, p < 0.001). These improvements were not accompanied by changes in plasma high-density lipoprotein cholesterol or plasma triglyceride levels. Our findings indicate that tannin-rich fiber from young persimmon fruits is a useful food material for treating hypercholesterolemia. Copyright © 2012 S. Karger AG, Basel.

News Article | February 23, 2017

Japan National Tourism Organization (JNTO) announces the 6th annual Japan Week, which will take place in Vanderbilt Hall at Grand Central Terminal from March 8-10, 2017. The exhibit, open to the public, showcases Japan as the ultimate travel destination while celebrating a unique fusion of traditional and modern Japanese culture, with the country’s high-tech futuristic products, local delicacies, and more. For Japan Week 2017, JNTO will feature a traditional Japanese Zen Garden as the centerpiece of the event to help promote Japanese tourism. The garden, complete with boulders, white gravels, moss and trees, will be directed by famous Japanese landscape designers. The Japan Week Zen Garden will showcase a large garden reminiscent of the Zen Garden from the Ryoan-ji Temple in Kyoto. Through the garden, the designers aim to tell the story of Oyashima, a Japanese origin story about ‘8 big islands’ to engage the public. JNTO hopes the garden will provide a peaceful backdrop at Grand Central Terminal Station to busy New Yorkers. The designers will create stone waves in the garden twice per day, which visitors are invited to watch. The 2017 Japan Week will promote a range of Japanese products and services where visitors are able to receive free Japanese tourism materials and travel tips from experts to learn about destinations throughout Japan, as well as experience the arts, crafts, food, and technology of Japanese culture. Exhibitors will showcase both the traditional and modern sides of Japan. Furthermore, Japan Week will introduce the story of Chiune Sugihara, the visa of life. The Japan Week 2017 exhibitors include: JR EAST / East Japan Railway Company, Kubara Honke USA,Inc.(KAYANOYA), FUJIFILM NORTH AMERICA CORPORATION, ANA (All Nippon Airways Co., Ltd.), JTB USA Inc., Gifu Prefectural Government, H.I.S. International Tours, Inc., Japan External Trade Organization (JETRO), Yamazaki USA Inc., ITO EN (North America) INC., Shiba Park Hotel Co., Ltd., Sugihara Route Promote Association, Tiger Corporation U.S.A., JAL Shopping Americas, Telecom Square USA, Inc., YAMASU, wuhao newyork Inc Tenugui Art, Amnet New York Travel, and IACE TRAVEL New York. Japan Week 2017 will also feature a Social Media Photo contest for the duration of the event. Attendees can participate by posting a photo at the event on their Instagram accounts with #JapanWeekNYC. Five winners will be selected and receive gift baskets of assorted products from some of the Japan Week exhibitors. Follow Japan Week on Facebook (, Twitter (, and Instagram (username@JapanWeekNY) for more details about the contest and join the conversation with #JapanWeekNYC. For more information about Japan Week’s exhibitors and activities, visit ABOUT JAPAN NATIONAL TOURISM ORGANIZATION (JNTO): As the official tourism board of Japan, JNTO is involved in a wide range of promotional activities encouraging international travelers to Japan. For more information on travel to Japan, go to

Matsumoto K.,Ishikawa Prefectural University | Yokoyama S.-I.,Gifu Prefectural Government
Food and Chemical Toxicology | Year: 2012

Kaki-tannin, a highly polymerized-tannin from the young fruits of persimmon (Diospyros kaki '. Hachiya'), has been shown to have bile acid-binding activity. To verify the effect of kaki-tannin on the metabolism of lipid and glucose in type 2 diabetes, type 2 diabetic NSY/Hos mice were fed an AIN76-modified high fat diet supplemented with 1% (w/w) kaki-tannin for 8. weeks. Kaki-tannin induced a 2-fold increase in fecal bile acid excretion and was significantly effective in the prevention of a rise in plasma cholesterol, triglyceride, and insulin levels. Kaki-tannin treatment also prevented fatty liver. To identify the molecular mechanism underlying these effects, gene expression analysis was performed on liver, brown adipose tissue (BAT), and skeletal muscle. The genes related to cholesterol metabolism, including 3-hydroxy-3-methylglutaryl-coenzyme A reductase and sterol regulatory element-binding protein 2, were increased in the liver of the kaki-tannin group. Interestingly, the uncoupling protein-1 (UCP1) gene and the UCP3 gene were significantly increased in the BAT of the kaki-tannin group, which was also confirmed at the protein level. These findings indicated that induction of UCP1 and UCP3 in the BAT by kaki-tannin treatment might influence the energy metabolism, thus contributing beneficial effects to type 2 diabetic NSY/Hos mice. © 2011 Elsevier Ltd.

Niwa T.,Nagoya University | Niwa T.,Shokei Gakuin University | Yokoyama S.-I.,Gifu Prefectural Government | Osawa T.,Nagoya University
Food Chemistry | Year: 2013

Soy isoflavonoids have many useful properties. However, they are metabolized in vivo, including in humans. The effect of the metabolism of soy isoflavonoids on their properties is not fully understood. We have isolated the bacterial strain SY8519, which has been shown to metabolize daidzein to O-desmethylangolensin and to produce 2-(4-hydroxyphenyl)propionic acid from genistein. According to chiral HPLC analysis, the 2-(4-hydroxyphenyl)propionic acid obtained from the bacterium was optically active. To determine the absolute stereochemistry of the microbial product, we prepared (S)-2-(4-hydroxyphenyl) propionic acid from (S)-2-phenylpropionic and concluded that the microbial product had an R-configuration by chiral HPLC analysis. We also applied the metabolite to mouse adipocytes and found that 2-HPPA was less effective at reducing leptin secretion than the parent compound genistein. Our results suggested that 'O-desmethylangolensin-production' attenuates the effect of soy isoflavonoids by reducing not only the activity of daidzein but also that of genistein. © 2012 Elsevier Ltd. All rights reserved.

PubMed | Aichi Gakuin University, Shokei Gakuin University and Gifu Prefectural Government
Type: | Journal: Food chemistry | Year: 2014

We had isolated an O-desmethylangolensin (O-DMA)-producing bacterium, Clostridium rRNA cluster XIVa strain SY8519. According to chiral separation using HPLC, the SY8519-produced O-DMA exhibited high optical purity. To determine the absolute stereochemistry of O-DMA, we prepared 2-(4-hydroxyphenyl)propionic acid (2-HPPA) from the O-DMA using the Baeyer-Villiger reaction. From chiral analysis of the product, the major peak had the same stereochemistry to that of 2-HPPA produced from genistein by the same bacteria. As we have determined the stereochemistry of SY8519-produced 2-HPPA to have an R configuration, by the chemical synthesis of (S)-2-HPPA, the SY8519-produced O-DMA must also possess R stereochemistry at the 2-position. To study the stereoselective metabolism, we applied racemic dihydrodaidzein to SY8519. The O-DMA was isolated from the culture media and starting material was also recovered. The O-DMA produced was optically active in a similar manner to that produced from daidzein. However, the remaining dihydrodaidzein exhibited no difference between the enantiomers. These results suggested that SY8519 produces (R)-O-DMA from both enantiomers of dihydrodaidzein.

PubMed | Gifu University, Aichi University and Gifu Prefectural Government
Type: Journal Article | Journal: Bioscience of microbiota, food and health | Year: 2016

Daidzein (DZN) is converted to equol (EQL) by intestinal bacteria. We previously reported that Eggerthella sp. YY7918, which is found in human feces, is an EQL-producing bacterium and analyzed its whole genomic sequence. We found three coding sequences (CDSs) in this bacterium that showed 99% similarity to the EQL-producing enzymes of Lactococcus sp. 20-92. These identified CDSs were designated eqlA, eqlB, and eqlC and thought to encode daidzein reductase (DZNR), dihydrodaidzein reductase (DHDR), and tetrahydrodaidzein reductase (THDR), respectively. These genes were cloned into pColdII. Recombinant plasmids were then introduced into Escherichia coli BL21 (DE3) and DZNR, DHDR, and THDR were expressed and purified by 6His-Tag chromatography. We confirmed that these three enzymes were involved in the conversion of DZN to EQL. Purified DZNR converted DZN to dihydrodaizein (DHD) in the presence of NADPH. DHDR converted DHD to tetrahydrodaizein (THD) in the presence of NADPH. Neither enzyme showed activities with NADH. THDR converted THD in the absence of cofactors, NAD(P)H, and also produced DHD as a by-product. Thus, we propose that THDR is not a reductase but a new type of dismutase. The GC content of these clusters was 64%, similar to the overall genomic GC content for Eggerthella and Coriobacteriaceae (56-60%), and higher than that for Lactococcus garvieae (39%), even though the gene cluster showed 99% similarity to that in Lactococcus sp. 20-92. Taken together, our results indicate that the gene cluster associated with EQL production evolved in high-GC bacteria including Coriobacteriaceae and was then laterally transferred to Lactococcus sp. 20-92.

Yokoyama S.-I.,Gifu Prefectural Government | Adachi Y.,Gifu Prefectural Government
Journal of Material Cycles and Waste Management | Year: 2015

We investigated the seasonal variations of organic materials and calorific values of the leaves of the Japanese cedar (Cryptomeria japonica) and the Japanese cypress (Chamaecyparis obtusa). While in April and May, the yields of alcohol–benzene extracts and Klason lignin of cedar and cypress leaves decreased; the yields of hemicellulose increased significantly compared with the annual average. The calorific values decreased simultaneously with the yields of alcohol–benzene extracts and Klason lignins of both types of leaves. The alcohol–benzene extracts yielded the highest calorific values (Japanese cedar, 33.4 ± 0.04 MJ/kg and Japanese cypress, 32.8 ± 0.37 MJ/kg), and hemicelluloses yielded the lowest calorific values of organic components (Japanese cedar, 12.8 ± 0.22 MJ/kg and Japanese cypress, 14.5 ± 1.02 MJ/kg). These results indicate that the changes of calorific values of these leaves are due to the changes of organic materials through the year. © 2015 Springer Japan

Si Q.,Mie University | Kawakubo M.,Mie University | Matsui M.,Mie University | Horiba T.,Mie University | And 4 more authors.
Journal of Power Sources | Year: 2014

Carbon coated silicon (Si/C) dispersed in a carbon paper (CP) was examined as the anode for solid polymer lithium-ion batteries. The CP was prepared by pyrolysis of poly(acrylonitrile) fiber and Manila hemp non-woven cloth at 2600 C under an inert atmosphere. The Si/C composite was formed by pyrolysis of a slurry consisting of Si power and a solution of polyvinyl chloride in tetrahydrofuran as the carbon source. Si/C:CP with a weight ratio of 20:100 had a high initial capacity of 980 mAh g-1 of Si/C and a high initial columbic efficiency of 77%, and also exhibited excellent capacity retention with a reversible capacity of 710 mAh g-1 of Si/C even after 250 cycles at a charge and discharge rate of 0.1 A g-1. The carbon fiber framework in the carbon paper could adsorb the volume change of Si during the lithium insertion and stripping processes. © 2013 Elsevier B.V. All rights reserved.

Si Q.,Mie University | Matsui M.,Mie University | Horiba T.,Mie University | Yamamoto O.,Mie University | And 3 more authors.
Journal of Power Sources | Year: 2013

We have developed a new Si-based anode for lithium secondary batteries consisting of a carbon paper substrate and Si/C composite deposited on it (Si/C/CP electrode). The Si/C composite was formed by pyrolysis of a slurry consisting of Si power and THF solution of PVC as the carbon precursor. The electrode contains no additional conductive carbon and binder, which supports the tight contact among the Si particle, pyrolytic carbon and carbon paper. The Si/C/CP electrode with the three-dimensional carbon fiber framework of the carbon paper was also advantageous to absorb severe volume changes of silicon particles. The electrode performance revealed that the Si/C/CP electrode prepared from the mixture of Si and PVC with the ratio of 10:90 by weight had a quite high initial capacity of 1720 mAh g-1 and initial Coulombic efficiency of 90%, and also exhibited excellent capacity retention with a reversible capacity of 1200 mAh g-1 even after 100 cycles. © 2013 The Authors. Published by Elsevier B.V. All rights reserved.

PubMed | Gifu Prefectural Government
Type: Journal Article | Journal: The Journal of general and applied microbiology | Year: 2013

Bacterial strain possessing both bacteriostatic and fungistatic activity (biocontrol activity) against pathogens of cyclamen (Cyclamen sp.) was isolated from the soil in Gifu Prefecture, Japan, and characterized with respect to its taxonomic and biocontrol properties. The sequence of its 16S rRNA gene, morphology, biochemistry, and fatty acid composition demonstrated that it is a strain most closely related to Alcaligenes faecalis subsp. faecalis LMG 1229(T). The isolate was named A. faecalis strain AD15. A. faecalis AD15 produced hydroxylamine at maximum yields of 33.31.7mg/L after 16 h cultivation in LB medium and 19.00.44mg/L after 19h cultivation in synthetic medium. Moreover, minimum inhibitory concentrations of hydroxylamine against the cyclamen pathogens Pantoea agglomerans and Colletotrichum gloeosporioides were 4.200.98 and 16.50.67mg/L. These results indicated that the biocontrol activity of strain AD15 might be attributed to hydroxylamine, a metabolite in the culture medium, and it had the potential for biopesticide application.

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