Purvi K.,Shivam Pharmaceutical Studies and Research Center |
Saluja Ajay K.,Gh Patel Institute Of Pharmacy
International Journal of Pharma and Bio Sciences | Year: 2014
The plant Trichodesma indicum R.Br. is easily available and distinctly used in inflammation. Whole plant is used as diuretic, as an emollient poultice, as a cure for fever, in ear pain. This study was undertaken to carry out pharmacological studies on aerial parts of plant. Methanolic extract and aqueous extract of aerial parts of Trichodesma indicum were used for screening diuretic activity using Lipschitz model. Diuretic activity, in view of urine volume, the methanolic extract at dose of 300 mg/kg has significant diuretic activity with lipschitz value 1.25 as compared to standard. Urinary sodium concentration was found to be more in methanolic extract but potassium was found to be more in aqueous extract. It also shows that, methanolic extract has effect like K+ sparing diuretics. It has also saluretic activity. In conclusion, Trichodesma indicum R.Br. possess significant diuretic activity these actions of Trichodesma indicum R.Br. can be attributed to Phytoconstituents present in it.
Patel R.B.,Gh Patel Institute Of Pharmacy |
Naregalkar N.S.,Gh Patel Institute Of Pharmacy
Journal of Liquid Chromatography and Related Technologies | Year: 2015
New high-performance thin layer chromatography (HPTLC) has been successfully developed and validated for quantitative estimation of Asenapine maleate in both marketed tablets and in-house developed formulations (solution, microemulsion, nanoemulsion, and mucoadhesive nanoemulsion) as well as for equilibrium solubility study and ex vivo diffusion study of developed formulation through natural membrane. As suggested by the International Conference on Harmonization, different stress test conditions (alkali, acid, thermal, photolytic, and humidity) were used to degrade Asenapine maleate. The samples produced from this study were utilized to develop a stability indicating HPTLC method. The Asenapine maleate was separated well from degradation products using HPTLC plate; precoated with silica gel G 60 F254 on aluminum sheet as a stationary phase and methanol as a mobile phase. Using densitometric analysis, Asenapine maleate was quantified at 235 nm. The method produced compact band for the drug (Rf = 0.43 + 0.02). The HPTLC method was validated and statistical analysis of the data confirmed the method to be specific, linear, accurate, precise, reproducible, and selective for Asenapine maleates analysis. This method was successfully used for assay of Asenapine maleate in both marketed tablets and in-house developed formulations, determining equilibrium solubility in various excipients as well as its ex vivo diffusion study through sheep nasal mucosa from the formulations developed in-house. © 2015 Taylor & Francis Group, LLC.
Patel R.B.,Gh Patel Institute Of Pharmacy |
Chaudhari M.D.,Gh Patel Institute Of Pharmacy
Journal of Liquid Chromatography and Related Technologies | Year: 2014
Stability-indicating high performance liquid chromatographic (HPLC) method for estimation of Adapalene was developed and validated. Adapalene was separated and quantitated on Nucleosil C8 column (250 mm length, 4.6 mm internal diameter, 5 lm particle size) using a blend of methanol-ammonium acetate buffer pH 4.0 (80:20 v/v) as a mobile phase and at a flow rate of 1. 3 mL/min. Quantification was achieved with ultraviolet (UV) detector at 270 nm over the concentration range 10-60 lg/mL. The applied HPLC method allowed separation and quantification of Adapalene with good linearity (r2= 0.999) in the studied concentration range. Limit of detection and limit of quantification were found to be 1.43 lg/mL and 4.34 lg/mL, respectively. The method was validated as per the International Conference on Harmonization (ICH) guidelines. Adapalene stock solution was subjected to different stress conditions. The degraded product peaks were well-resolved from the pure drug peak with significant difference in their retention time values. Stressed samples were assayed using developed HPLC method. Statistical analysis of the data showed that the method is precise, accurate, reproducible, and selective for the analysis of Adapalene. The method was successfully applied to the estimation of Adapalene in tablet dosage forms. © Taylor & Francis Group, LLC.
Mehta F.A.,P.A. College |
Patel B.G.,Gh Patel Institute Of Pharmacy |
Ahir K.B.,P.A. College
Journal of Planar Chromatography - Modern TLC | Year: 2011
Achyranthes aspera L. var aspera is much valued in the ayurvedic system of medicine. It is also used as a medicinal plant in other countries. Literature review and chemical investigations in our laboratory showed the presence of alkaloids and triterpenoid saponins. Two alcohol extracts of the plant were analyzed to determine amounts of the alkaloid betaine. Compounds were separated on silica gel 60 F254 plates with methanol-water 9:1 (ν/ν) as mobile phase. Detection by measurement of absorbance at 550 nm after derivatization with modified Dragendorff's reagent. The RF value was 0.4. The betaine content of the extracts was calculated statistically. Comparison was made with betaine standard. The method was accurate, precise, repeatable, and reproducible. The linear range of the method was 4-30 μg per band. The robustness and ruggedness of the method was also evaluated. The technique may be used for routine standardization of the betaine content of alcohol extracts of A. aspera. © Akadémiai Kiadó, Budapest.
Khandekar S.,Veer Narmad South Gujarat University |
Dewaliya Y.,Disha Life science Pvt. Ltd. |
Trivedi N.D.,Gh Patel Institute Of Pharmacy |
Trivedi U.N.,Shivam Pharmaceutical Studies and Research Center
American Journal of Biochemistry and Molecular Biology | Year: 2014
The present study reports on the isolation and characterization of new isolates from crude oil from petroleum contaminated soil. The isolates were then screened for their potential towards biodegradation of toluene by growing them in both solid as well as liquid mineral medium supplemented with toluene as a sole carbon source and further identified by GC-FAME analysis. The degradation potential of these isolates strongly suggests that the isolates have TOL like plasmid and carries xylM gene involved in the expression of the toluene monooxygenase. The primer sets were used in PCR to assess the presence of the catabolic gene in new toluene degrading isolates. The primer verification was done by using bioinformatics tools. From the results it is conclusive that isolates were able to degrade Toluene, and from PCR it is evident that the isolates contained gene coding for Toluene degradation. The test results indicate that the bacteria could contribute to bioremediation of aromatic hydrocarbon pollution. © Academic Journals Inc.
Galani V.,Gh Patel Institute Of Pharmacy |
Patel B.,Gh Patel Institute Of Pharmacy |
Patel N.,Gh Patel Institute Of Pharmacy
Pharmacognosy Reviews | Year: 2010
Argyreia speciosa (Linn. f.) Sweet is a popular Indian medicinal plant, which has long been used in traditional Ayurvedic Indian medicine for various diseases. This plant is pharmacologically studied for nootropic, aphrodisiac, immunomodulatory, hepatoprotective, antioxidant, antiinflammatory, antihyperglycemic, antidiarrheal, antimicrobial, antiviral, nematicidal, antiulcer, anticonvulsant, analgesic and central nervous depressant activities. A wide range of phytochemical constituents have been isolated from this plant. A comprehensive account of the morphology, phytochemical constituents and pharmacological activities reported are included in view of the many recent findings of importance on this plant.
Patel A.S.,Gh Patel Institute Of Pharmacy |
Saikat P.,Gh Patel Institute Of Pharmacy |
Pravinbhai P.R.,Gh Patel Institute Of Pharmacy
Current Drug Delivery | Year: 2014
The spherical microspheres consisting of Furosemide loaded sodium alginate and along with HPMC E50 or sodium CMC as mucoadhesive polymers in different ratios were prepared using ionic gelation technique. Calcium chloride was used as crosslinking, to retard the drug release from the mucoadhesive microspheres. The prepared mucoadhesive microspheres were subjected for evaluation of various parameters like production yield, particle size, encapsulation efficiency, mucoadhesion test and in vitro dissolution profile studies. Formulations were subjected to DSC study and SEM analysis. The in vitro release data were well fit into Higuchi and Korsmeyer-Peppas model and followed non-Fickian diffusion mechanism. © 2014 Bentham Science Publishers.
Prajapati P.,Gujarat Forensic Sciences University |
Vaghela V.,Gh Patel Institute Of Pharmacy |
Rawtani D.,Gujarat Forensic Sciences University |
Patel H.,Gujarat Forensic Sciences University |
And 2 more authors.
Journal of Pharmaceutical Analysis | Year: 2012
A novel, safe, economic and sensitive method of spectrophotometric estimation has been developed using Azeoptropic mixture (water:methanol: 60:40, v/v) for the quantitative determination of Lornoxicam, a practically water-insoluble drug. Hence, Lornoxicam stock solution was prepared in Azeoptropic mixture. Lornoxicam showed maximum absorbance at 383 nm. Beers law was obeyed in the concentration range 4-24 μg/mL with regression coefficient of 0.999. The method was validated in terms of linearity (R2=0.999), precision (CV for intra-day and inter-day was 0.28-0.68 and 0.12-0.92, respectively), accuracy (98.03-100.59% w/w) and specificity. This method is simple, precise, accurate, sensitive and reproducible and can be used for the routine quality control testing of the marketed formulations. © 2012 Xi'an Jiaotong University.
Prajesh P.,Gujarat Forensic Sciences University |
Vipul V.,Gh Patel Institute Of Pharmacy
Journal of Liquid Chromatography and Related Technologies | Year: 2013
Ciclesonide, 2-[(1S,2S,4 R,8S,9S,11S,12S,13 R)-6-cyclohexyl-11-hydroxy-9, 13-dimethyl-16-oxo-5,7-dioxapentacyclo[10.8.0.02,9.04,8.013,18]icosa-14, 17-dien-8-yl]-2-oxoethyl 2-methyl propanoate, persists as a newer anti-asthmatic drug of the glucocorticoid class. A fast, safe, and sensitive method for estimation of Ciclesonide was developed as it is officially in IP-2007 and acquires a longer time for detection. The chromatographic separation employs isocratic elution by utilizing an inertsil ODS-C18, 250 mm × 4.6 mm, 5 μm columns. A mobile phase consisting of solvent [solution containing Methanol:Water (95:05%, v/v)] endowed at a flow rate of 1.0 mL min-1. The analyte was detected and quantified at λmax 242 nm using UV detector. The method was validated according to ICH guidelines, illustrating to be accurate (98.91%-100.59%) and precise (CV = 0.21-1.11 for intra-day and CV = 0.21-1.51 for inter-day) within the corresponding linear range. © 2013 Taylor and Francis Group, LLC.
Prajapati P.,Gujarat Forensic Sciences University |
Vaghela V.,Gh Patel Institute Of Pharmacy
Journal of Planar Chromatography - Modern TLC | Year: 2013
A sensitive method based on high-performance thin-layer chromatography (HPTLC) by using principle of densitometry with UV detection at λmax 242 nm for quantitative estimation of major pharmaceutically active compound, ciclesonide, 2-[(1S,2S,4R,8S,9S, 11S,12S,13R)-6-cyclohexyl-11-hydroxy-9,13-dimethyl-16-oxo-5,7- dioxapentacyclo[10.8.0.02,9.04,8.013,18]icosa-14,17-dien-8-yl]-2-oxoethyl-2-methylpropanoate, which persists as a newer antiasthmatic drug of glucocorticoid class, is described here. Chromatographic separation was performed by semiautomatic CAMAG HPTLC instrument. The method was validated according to the International Conference on Harmonization (ICH) guidelines, illustrating to be accurate (98.92-100.26%) and precise (CV = 0.43-0.67 for intra-day and CV = 0.58-1.45 for inter-day) within the corresponding linear range. Limit of detection (LOD) and limit of quantification (LOQ) of sample were found to be 100 ng/spot and 303 ng/spot, respectively. © Akadémiai Kiadó, Budapest.