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Tiwari A.K.,UP Council of Sugarcane Research | Kumari K.,UP Council of Sugarcane Research | Tiwari N.N.,UP Council of Sugarcane Research | Kumar P.,Ben - Gurion University of the Negev | And 2 more authors.
Archives of Phytopathology and Plant Protection | Year: 2016

During the survey of two successive years 2012–2013, in nearby places of Gorakhpur districts, Uttar Pradesh, India, Arundo donax plants were found to be exhibiting witches’ broom, excessive branching accompanied with little leaf symptoms with considerable disease incidence. Nested PCR carried out with universal primers pair R16F2n/R16R2 employing the PCR (P1/P7) product as a template DNA (1:20) resulted in expected size positive amplification ~1.2 kb in all symptom-bearing plants suggested the association of phytoplasma with witches’ broom disease of Narkat plants. BLASTn analysis of the 16S rRNA gene sequence showed the highest (99%) sequence identity with Candidatus phytoplasma asteris (16SrI group). In phylogenetic analysis, the sequence data showed close relationships with the members of 16SrI phytoplasma and clustered within a single clade of 16SrI group and closed to B subgroup representatives. This is a first report of 16Sr I-B group phytoplasma associated with witches’ broom accompanied with little leaf disease of Narkat in India. © 2016 Taylor & Francis Source


Kumar R.,Inter University Accelerator Center | Sharma V.R.,Am University | Yadav A.,Inter University Accelerator Center | Singh P.P.,IIT | And 7 more authors.
Acta Physica Polonica B | Year: 2015

Aiming to investigate the incomplete fusion processes at low projectile energies, experiments have been carried out for the 14N + 169Tm system at ≈ 4-7 MeV/A. Excitation functions for several residues likely to be populated via complete and incomplete fusion processes have been measured for the first time using heavy recoil residue catcher technique followed by -ray spectroscopy. The measured excitation functions are compared with the calculations based on the available statistical model codes. The incomplete fusion strength function is found to increase with the projectile energy. An attempt has been made to compare the present system with an semi-empirical code MARC for incomplete fusion. Source


A simple, precise, rapid, selective, (HPTLC) method has been established for simultaneous analysis of Domperidone (DMP) and paractamol (PAR) in tablet dosage forms. The Chromatographic separation was performed on per coated silica gel 60 GF254. plate with acetone-methanol-carbon tetrachloride 5:5:1 (V/V) as mobile phase. The plates were developed to a distance of 8.0 cm at ambient temperature. The developed plate were scanned and quantified at their single wavelength of maximum absorption at approximately 280 and 245nm for Domperidone and paractamol, respectively. Experimental conditions such as band size, chamber saturation time, migration of solvent front, slit width, etc. were critically studied and the optimum condition were selected. The drugs were satisfactorily resolved with Rf 0.53±0.02 for domperidone and 0.73±0.02 for paracetamol. The method was validated for linearity, accuracy, precision and specificity. The calibration plot was linear between 15-45 ng per band for DMP and 850-2500 ng per band for PAR. The limit of detection and quantification for DMP were 0.23 and 0.189 ng per band, respectively; for PAR they were 0.308 and 0.938 ng per band. This HPTLC procedure is economic, sensitive, and less time consuming than other Chromatographic procedures. It is a user-friendly and important tool for analysis of combined tablets forms. Source


Kumar P.,GF College | Agarwal A.,GF College | Tiwari A.K.,UP Council of Sugarcane Research | Lal M.,UP Council of Sugarcane Research | Jabri M.R.A.,GF College
Sugar Tech | Year: 2012

Red rot behavior of 50 somaclones of sugarcane variety CoS 88230, was studied by plug and nodal methods of inoculation using a mixture of four isolates of Colletotrichum falcatum. The results revealed that most of the somaclones had red rot susceptibility to varying degrees except four somaclones viz. SC-15, 20, 22 and 30, which were found moderately resistant (MR) by plug method of inoculation. Two somaclones viz. SC-22 and 30 were found resistant by nodal method of inoculation. Using plug method of inoculation, the aforesaid four somaclones viz. SC-15, 20, 22 and 30 were also found moderately resistant against all the four strains if tested individually, according to 0-9 scale except against isolate R 8411. These somaclones were better in red rot resistance than the donor variety CoS 88230. The wide variations observed in a number of traits along with red rot behavior in the somaclonal population was possibly due to complex genetic nature and existence of chromosomal mosaicism in commercial hybrid varieties of sugarcane. © 2012 Society for Sugar Research & Promotion. Source


The complexes of the type MLXn where M=Co(III),Fe(III), Mn(III), Ti(III),V(III), Ru(III),Ru(II) MoO(V),MoO2(VI) and UO 2(VI),X=CI/NO3, n=2/4, and L is the thiosemicarbazide ligand derived from reaction between benzofuran-2-carboxy hydrazide and phenylisothiocyanate(BCPT)were prepared. All the complexes isolated in solid state, are stable in air and characterized by the elemental analyses, IR, electronic spectral data, magnetic moment and conductance measurement studies. Thiosemicarbazide ligand behaves as bidentate by co-ordinating through oxygen of carbonyl group and nitrogen of hydrazine residue. The probable structure for all the metal complexes are also proposed here. The thiosemicarbazide ligand and its metal complexes have been screened for their antimicrobial activity. Source

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