Englewood, CO, United States
Englewood, CO, United States

Gevo, Inc. is a renewable chemicals and advanced biofuels company headquartered in unincorporated Douglas County, Colorado in the Denver-Aurora metropolitan area. The company develops bio-based alternatives to petroleum-based products using a combination of biotechnology and classical chemistry. Gevo converts renewable raw materials into isobutanol and renewable hydrocarbons that Gevo believes can be directly integrated on a “drop in” basis into existing fuel and chemical products. Gevo’s investors include Burrill & Company, Khosla Ventures, Lanxess, Osage University Partners, Total, and Virgin Green Fund, among others. Wikipedia.


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Patent
Toray Industries Inc and Gevo | Date: 2014-04-23

[Problem] To provide a method of producing a biomass resource-derived polyester capable of greatly reducing fossil resource usage amounts and carbon dioxide increases that is in way inferior in color or thermal stability to a conventional fossil resource-derived product while also having superior dye affinity. [Solution] The biomass resource-derived dicarboxylic acid and/or ester forming derivative thereof used as the raw material fulfills at least one of the following conditions:(A) The amount of potassium hydroxide needed to neutralize an acid component extracted using an organic solvent from 1 g of the dicarboxylic acid and/or ester forming derivative thereof is 0.1 mg KOH/g or less(B) The amount of potassium hydroxide needed to neutralize an acid component extracted using water from 1 g of the dicarboxylic acid and/or ester forming derivative thereof is 0.05 mg KOH/g or less(C) Sulfuric acid ion content is 40 ppm or less


This invention is directed to methods for recovery of C3-C6 alcohols from dilute aqueous solutions, such as fermentation broths. Such methods provide improved volumetric productivity for the fermentation and allows recovery of the alcohol. Such methods also allow for reduced energy use in the production and drying of spent fermentation broth due to increased effective concentration of the alcohol product by the simultaneous fermentation and recovery process which increases the quantity of alcohol produced and recovered per quantity of fermentation broth dried. Thus, the invention allows for production and recovery of C3-C6 alcohols at low capital and reduced operating costs.


The present invention provides recombinant microorganisms comprising isobutanol producing metabolic pathway with at least one isobutanol pathway enzyme localized in the cytosol, wherein said recombinant microorganism is selected to produce isobutanol from a carbon source. Methods of using said recombinant microorganisms to produce isobutanol are also provided. In various aspects of the invention, the recombinant microorganisms may comprise a cytosolically active isobutanol pathway enzymes. In some embodiments, the invention provides mutated, modified, and/or chimeric isobutanol pathway enzymes with cytosolic activity. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.


Patent
Gevo | Date: 2014-11-03

The present invention is directed to renewable compositions derived from fermentation of biomass, and integrated methods of preparing such compositions.


This invention is directed to methods for recovery of C3-C6 alcohols from dilute aqueous solutions, such as fermentation broths. Such methods provide improved volumetric productivity for the fermentation and allow recovery of the alcohol. Such methods also allow for reduced energy use in the production and drying of spent fermentation broth due to increased effective concentration of the alcohol product by the simultaneous fermentation and recovery process which increases the quantity of alcohol produced and recovered per quantity of fermentation broth dried. Thus, the invention allows for production and recovery of C3-C6 alcohols at low capital and reduced operating costs.


The present invention provides recombinant microorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms may comprise a modification resulting in the reduction of pyruvate decarboxylase and/or glycerol-3-phosphate dehydrogenase activity. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.


Isobutene, isoprene, and butadiene are obtained from mixtures of C_(4 )and/or C_(5 )olefins by dehydrogenation. The C_(4 )and/or C_(5 )olefins can be obtained by dehydration of C_(4 )and C_(5 )alcohols, for example, renewable C_(4 )and C_(5 )alcohols prepared from biomass by thermochemical or fermentation processes. Isoprene or butadiene can be polymerized to form polymers such as polyisoprene, polybutadiene, synthetic rubbers such as butyl rubber, etc. in addition, butadiene can be converted to monomers such as methyl methacrylate, adipic acid, adiponitrile, 1,4-butadiene, etc. which can then be polymerized to form nylons, polyesters, polymethylmethacrylate etc.


The present invention is directed to recombinant microorganisms comprising one or more dihydroxyacid dehydratase (DHAD)-requiring biosynthetic pathways and methods of using said recombinant microorganisms to produce beneficial metabolites derived from said DHAD-requiring biosynthetic pathways. In various aspects of the invention, the recombinant microorganisms may be engineered to overexpress one or more polynucleotides encoding one or more Aft proteins or homologs thereof. In some embodiments, the recombinant microorganisms may comprise a cytosolically localized DHAD enzyme. In additional embodiments, the recombinant microorganisms may comprise a mitochondrially localized DHAD enzyme. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.


Methods for producing a biofuel are provided. Also provided are biocatalysts that convert a feedstock to a biofuel.


The present invention relates to recombinant microorganisms comprising biosynthetic pathways and methods of using said recombinant microorganisms to produce various beneficial metabolites. In various aspects of the invention, the recombinant microorganisms may further comprise one or more modifications resulting in the reduction or elimination of 3 keto-acid (e.g., acetolactate and 2-aceto-2-hydroxybutyrate) and/or aldehyde-derived by-products. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces Glade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.

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