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PubMed | Germany German Rheumatism Research Center, Bielefeld University, Chinese PLA General Hospital, Dokuz Eylül University and 21 more.
Type: Journal Article | Journal: Annals of the rheumatic diseases | Year: 2016

To establish the predictive validity of the Assessment of SpondyloArthritis international Society (ASAS) spondyloarthritis (SpA) classification criteria.22 centres (N=909 patients) from the initial 29 ASAS centres (N=975) participated in the ASAS-cohort follow-up study. Patients had either chronic (>3months) back pain of unknown origin and age of onset below 45years (N=658) or peripheral arthritis and/or enthesitis and/or dactylitis (N=251). At follow-up, information was obtained at a clinic visit or by telephone. The positive predictive value (PPV) of the baseline classification by the ASAS criteria was calculated using rheumatologists diagnosis at follow-up as external standard.In total, 564 patients were assessed at follow-up (345 visits; 219 telephone) with a mean follow-up of 4.4years (range: 1.9; 6.8) and 70.2% received a SpA diagnosis by the rheumatologist. 335 patients fulfilled the axial SpA (axSpA) or peripheral SpA (pSpA) criteria at baseline and of these, 309 were diagnosed SpA after follow-up (PPV SpA criteria: 92.2%). The PPV of the axSpA and pSpA criteria was 93.3% and 89.5%, respectively. The PPV for the clinical arm only was 88.0% and for the clinical armimaging arm 96.0%, for the imaging arm only 86.2% and for the imaging arm+/-clinical arm 94.7%. A series of sensitivity analyses yielded similar results (range: 85.1-98.2%).The PPV of the axSpA and pSpA criteria to forecast an experts diagnosis of SpA after more than 4years is excellent. The imaging arm and clinical arm of the axSpA criteria have similar predictive validity and are truly complementary.


Radbruch H.,Charite University of Medicine | Bremer D.,Germany German Rheumatism Research Center | Mothes R.,Charite University of Medicine | Mothes R.,Germany German Rheumatism Research Center | And 9 more authors.
International Journal of Molecular Sciences | Year: 2015

The development of intravital Förster Resonance Energy Transfer (FRET) is required to probe cellular and tissue function in the natural context: the living organism. Only in this way can biomedicine truly comprehend pathogenesis and develop effective therapeutic strategies. Here we demonstrate and discuss the advantages and pitfalls of two strategies to quantify FRET in vivo—ratiometrically and time-resolved by fluorescence lifetime imaging—and show their concrete application in the context of neuroinflammation in adult mice. © 2015 by the authors; licensee MDPI, Basel, Switzerland.


PubMed | Charite University of Medicine and Germany German Rheumatism Research Center
Type: Journal Article | Journal: International journal of molecular sciences | Year: 2015

The development of intravital Frster Resonance Energy Transfer (FRET) is required to probe cellular and tissue function in the natural context: the living organism. Only in this way can biomedicine truly comprehend pathogenesis and develop effective therapeutic strategies. Here we demonstrate and discuss the advantages and pitfalls of two strategies to quantify FRET in vivo-ratiometrically and time-resolved by fluorescence lifetime imaging-and show their concrete application in the context of neuroinflammation in adult mice.

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