Radbruch H.,Charite University of Medicine |
Bremer D.,Germany German Rheumatism Research Center |
Mothes R.,Charite University of Medicine |
Mothes R.,Germany German Rheumatism Research Center |
And 9 more authors.
International Journal of Molecular Sciences | Year: 2015
The development of intravital Förster Resonance Energy Transfer (FRET) is required to probe cellular and tissue function in the natural context: the living organism. Only in this way can biomedicine truly comprehend pathogenesis and develop effective therapeutic strategies. Here we demonstrate and discuss the advantages and pitfalls of two strategies to quantify FRET in vivo—ratiometrically and time-resolved by fluorescence lifetime imaging—and show their concrete application in the context of neuroinflammation in adult mice. © 2015 by the authors; licensee MDPI, Basel, Switzerland. Source