Geriatric Research Center Clinical Core

East Palestine, OH, United States

Geriatric Research Center Clinical Core

East Palestine, OH, United States

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Van Epps P.,Geriatric Research Center Clinical Core | Van Epps P.,Case Western Reserve University | Banks R.,Geriatric Research Center Clinical Core | Aung H.,Geriatric Research Center Clinical Core | And 4 more authors.
Immunity and Ageing | Year: 2014

Background: A reduced number of naïve T cells along with an accumulation of differentiated cell types in aging have been described but little is known about the polyfunctionality of the T cell responses. In this study we compared the individual and polyfunctional expression of IFN-γ, MIP-1α, TNF-α, perforin, and IL-2 by T cell subsets, including the newly described stem cell like memory T cells (TSCM), in response to stimulation with superantigen staphylococcal enterotoxin B (SEB) in older (median age 80, n = 23) versus younger (median age 27; n = 23) adults. Results: Older age was associated with a markedly lower frequency of CD8+ naïve T cells (11% vs. 47%; p < 0.0001) and an expansion in memory T cell subsets including central memory (p < 0.05), effector memory and effector T cells (p < 0.001 for both). There was also a decline in CD4+ naïve T cells in older subjects (33% vs. 45%; p = 0.02). There were no differences in frequencies or polyfunctional profiles of TSCM between groups. CD8+ naïve cells in the older group had increased expression of all functional parameters measured compared to the younger subjects and exhibited greater polyfunctionality (p = 0.04). CD4+ naïve T cells in the older group also showed greater polyfunctionality with a TNF-α and IL-2 predominance (p = 0.005). CD8+ effector memory and effector T cells exhibited increased polyfunctionality in the older group compared with younger (p = 0.01 and p = 0.003). Conclusions: These data suggest that aging does not have a negative effect on polyfunctionality and therefore this is likely not a major contributor to the immunesenescence described with aging. © Van Epps et al.; licensee BioMed Central Ltd.


PubMed | University of Pennsylvania, Case Western Reserve University and Geriatric Research Center Clinical Core
Type: | Journal: Immunity & ageing : I & A | Year: 2014

A reduced number of nave T cells along with an accumulation of differentiated cell types in aging have been described but little is known about the polyfunctionality of the T cell responses. In this study we compared the individual and polyfunctional expression of IFN-, MIP-1, TNF-, perforin, and IL-2 by T cell subsets, including the newly described stem cell like memory T cells (TSCM), in response to stimulation with superantigen staphylococcal enterotoxin B (SEB) in older (median age 80, n=23) versus younger (median age 27; n=23) adults.Older age was associated with a markedly lower frequency of CD8+ nave T cells (11% vs. 47%; p<0.0001) and an expansion in memory T cell subsets including central memory (p<0.05), effector memory and effector T cells (p<0.001 for both). There was also a decline in CD4+ nave T cells in older subjects (33% vs. 45%; p=0.02). There were no differences in frequencies or polyfunctional profiles of TSCM between groups. CD8+ nave cells in the older group had increased expression of all functional parameters measured compared to the younger subjects and exhibited greater polyfunctionality (p=0.04). CD4+ nave T cells in the older group also showed greater polyfunctionality with a TNF- and IL-2 predominance (p=0.005). CD8+ effector memory and effector T cells exhibited increased polyfunctionality in the older group compared with younger (p=0.01 and p=0.003).These data suggest that aging does not have a negative effect on polyfunctionality and therefore this is likely not a major contributor to the immunesenescence described with aging.

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