Entity

Time filter

Source Type


Favoretto S.R.,Instituto Pasteur Of Sao Paulo | Favoretto S.R.,University of Sao Paulo | Labruna M.B.,University of Sao Paulo | Campos A.C.A.,University of Sao Paulo | And 3 more authors.
Revista da Sociedade Brasileira de Medicina Tropical | Year: 2013

Introduction: This study assessed the viability of the rabies virus in the argasid tick Carios fonsecai following experimental infection. Methods: The mouse inoculation test (MIT), fluorescent antibody test (FAT) and polymerase chain reaction (PCR) were used. The rabies virus was administered to ticks via the intra-coelomic route, and the ticks were sacrificed at different time points. Results: The inoculated ticks were negative for rabies according to the MIT. Ticks macerated with rabies virus were positive according to the MIT and FAT. All of the tick lots tested by PCR were positive. Conclusions: The rabies virus became unviable shortly after its inoculation into tick bodies. Ticks are not likely to play an important role in the epidemiology of rabies. Source


Purps J.,Charite - Medical University of Berlin | Siegert S.,University of Cologne | Willuweit S.,Charite - Medical University of Berlin | Nagy M.,Charite - Medical University of Berlin | And 163 more authors.
Forensic Science International: Genetics | Year: 2014

In a worldwide collaborative effort, 19,630 Y-chromosomes were sampled from 129 different populations in 51 countries. These chromosomes were typed for 23 short-tandem repeat (STR) loci (DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS385ab, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS635, GATAH4, DYS481, DYS533, DYS549, DYS570, DYS576, and DYS643) and using the PowerPlex Y23 System (PPY23, Promega Corporation, Madison, WI). Locus-specific allelic spectra of these markers were determined and a consistently high level of allelic diversity was observed. A considerable number of null, duplicate and off-ladder alleles were revealed. Standard single-locus and haplotype-based parameters were calculated and compared between subsets of Y-STR markers established for forensic casework. The PPY23 marker set provides substantially stronger discriminatory power than other available kits but at the same time reveals the same general patterns of population structure as other marker sets. A strong correlation was observed between the number of Y-STRs included in a marker set and some of the forensic parameters under study. Interestingly a weak but consistent trend toward smaller genetic distances resulting from larger numbers of markers became apparent. © 2014 The Authors. Source


Favaro E.C.,Genomic Engineering Molecular Ltda | Sumita D.R.,Genomic Engineering Molecular Ltda | Whittle M.R.,Genomic Engineering Molecular Ltda
Forensic Science International: Genetics Supplement Series | Year: 2011

At present forensic identification and parentage testing is invariably carried out using multiplex PCR amplification kits in which many loci, whether they be STRs, SNPs or DIPs are analyzed simultaneously using primers labelled with at least three, but usually four, different fluorophores. Should existing loci need to be substituted by newer, for instance, more polymorphic loci, new fluorescent primers need to be synthesized; indeed, the dye labels of several loci may need to be switched to allow a re-accommodation of the allele sizes in the single multiplex, and this can be costly. We sought to facilitate these substitutions and reduce costs by using four universal primers each labelled with a different fluorophore and employ these in a test multiplex PCR amplification of 20 DIP loci. © 2011 Elsevier Ireland Ltd. Source


Francischini C.W.,Genomic Engineering Molecular Ltda | Sumita D.R.,Genomic Engineering Molecular Ltda | Whittle M.R.,Genomic Engineering Molecular Ltda
Forensic Science International: Genetics Supplement Series | Year: 2013

We describe the development and use of single tube multiplex PCR kit in which 21 commonly used autosomal STR loci, together with the amelogenin locus, are amplified. For relationship testing the amplification occurs directly from a single 1. mm filter paper disk containing dried blood or saliva, and the loci are amplified using primers with only four different fluorophores. The alleles are separated and visualized using standard capillary DNA sequencers. An allelic ladder comprising the most prevalent alleles of the 21 loci in the Brazilian population is also used to control for electrophoretic mobility. © 2013 Elsevier Ireland Ltd. Source


Whittle M.R.,Genomic Engineering Molecular Ltda | Favaro E.C.,Genomic Engineering Molecular Ltda | Francischini C.W.,Genomic Engineering Molecular Ltda | Sumita D.R.,Genomic Engineering Molecular Ltda
Forensic Science International: Genetics Supplement Series | Year: 2011

Most laboratories presently carrying out forensic identification and parentage testing use capillary DNA sequencers to separate and visualize the alleles that are produced by multiplex PCR amplification, whether they be of STR, SNP or DIP loci. Because at least four, but usually five, different fluorophores are used simultaneously, each instrument must undergo its own spectral calibration in which a matrix is created and used to correct the overlapping of fluorescence emission spectra of the mixture of dyes. For this users must acquire matrix standards in addition to the multiplex PCR kits and it is not clear what these standards consist of. In our efforts to develop new multiplex STR and DIP amplification kits using different fluorophores from those commonly used, we were obliged to produce new matrix standards to calibrate our multicapillary sequencers, and we describe how this was done. © 2011 Elsevier Ireland Ltd. Source

Discover hidden collaborations