Genome Corp.

Providence, RI, United States

Genome Corp.

Providence, RI, United States
SEARCH FILTERS
Time filter
Source Type

Kim M.Y.,Chungbuk National University | Lee S.H.,Chungbuk National University | Jang G.Y.,Chungbuk National University | Park H.J.,Chungbuk National University | And 6 more authors.
Food Chemistry | Year: 2015

This study was performed to evaluate the enhancement of functional components of germinated rough rice. Rough rice was germinated at 37 °C for 6 days, and subjected to a high hydrostatic pressure treatment (HPT) at 30 MPa for 24 h (HP24) and 48 h (HP48). Germinated rough rice without HPT (HP0), HP24, and HP48 were analysed for their functional components. The highest γ-aminobutyric acid, total arabinoxylan, and tricin 4′-O-(threo- β-guaiacylglyceryl) ether contents were 121.21 mg/100 g, 10.6%, and 85.82 μg/g, respectively, after HP48 for 2 days. γ-Oryzanol contents increased from 23.19-36.20 mg/100 g (at HP0) to 31.80-40.32 mg/100 g (at HP48). The highest vitamin B (60.99 mg/100 g) and E (4.07 mg/100 g) contents were observed after HP24 for 5 and 2 days, respectively. These results suggest that a combination of HPT and germination efficiently enhances the functional characteristics of rough rice. © 2014 Elsevier Ltd. All rights reserved.


Saminathan T.,West Virginia State University | Nimmakayala P.,West Virginia State University | Manohar S.,West Virginia State University | Malkaram S.,West Virginia State University | And 12 more authors.
Journal of Experimental Botany | Year: 2015

The exploitation of synthetic polyploids for producing seedless fruits is well known in watermelon. Tetraploid progenitors of triploid watermelon plants, compared with their diploid counterparts, exhibit wide phenotypic differences. Although many factors modulate alternative splicing (AS) in plants, the effects of autopolyploidization on AS are still unknown. In this study, we used tissues of leaf, stem, and fruit of diploid and tetraploid sweet watermelon to understand changes in gene expression and the occurrence of AS. RNA-sequencing analysis was performed along with reverse transcription quantitative PCR and rapid amplification of cDNA ends (RACE)-PCR to demonstrate changes in expression and splicing. All vegetative tissues except fruit showed an increased level of AS in the tetraploid watermelon throughout the growth period. The ploidy levels of diploids and the tetraploid were confirmed using a ploidy analyser. We identified 5362 and 1288 genes that were up- and downregulated, respectively, in tetraploid as compared with diploid plants. We further confirmed that 22 genes underwent AS events across tissues, indicating possibilities of generating different protein isoforms with altered functions of important transcription factors and transporters. Arginine biosynthesis, chlorophyllide synthesis, GDP mannose biosynthesis, trehalose biosynthesis, and starch and sucrose degradation pathways were upregulated in autotetraploids. Phloem protein 2, chloroplastic PGR5-like protein, zinc-finger protein, fructokinase-like 2, MYB transcription factor, and nodulin MtN21 showed AS in fruit tissues. These results should help in developing high-quality seedless watermelon and provide additional transcriptomic information related to other cucurbits. © The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved.


Kim M.Y.,Chungbuk National University | Lee S.H.,Chungbuk National University | Jang G.Y.,Chungbuk National University | Park H.J.,Chungbuk National University | And 5 more authors.
Journal of the Korean Society of Food Science and Nutrition | Year: 2013

This study investigated the enzyme inhibitory effects of ethanol extracts from the different parts of rough rice (Oryza sativar L.) from its germination period. Rough rice was germinated at 37°C for 6 days, then separated into hull+sprout and brown rice. α-Glucosidase inhibitory activity had the highest value (39.38%) in hull+sprout extracts after 5 days of germination. α-Amylase and DPP-4 inhibitory activity had the highest values (75.32% and 47.77%, respectively) in hull+sprout extracts after germination for 5 days. ACE inhibitory activity of hull+ sprout extracts increased from 43.16% at the beginning to 58.60% at 5 days, while brown rice extracts increased this activity from 0.88% at the beginning to 14.50% at 4 days. The xanthine oxidase inhibitory activity of hull+ sprout extracts increased from 62.02% at the beginning to 64.49% at one day, and then decreased. Lipase inhibitory activity had its highest value (55.17%) in hull+sprout extracts after germination for 5 days. These results indicate that the optimal germination period for increasing enzyme inhibitory activities may be 5 days, and that hull+sprout extracts have a higher enzyme inhibition activity than brown rice.


Kim M.Y.,Chungbuk National University | Lee S.H.,Chungbuk National University | Jang G.Y.,Chungbuk National University | Park H.J.,Chungbuk National University | And 5 more authors.
Journal of the Korean Society of Food Science and Nutrition | Year: 2013

This study is investigated to evaluate the enhancement of antioxidant compound and activity of rough rice with different germination periods and high pressure treatment. The subject was germinated at 37°C for 6 days (HP0), and then the germinated rough rice were subjected to 30 MPa for 24 hr (HP24) and 48 hr (HP48), respectively. HP0, HP24 and HP48 samples were prepared and extracted with 80% ethanol. The highest total polyphenol contents (5.15 mg/g) occurred in treating at HP48 after germination for 5 days. The total phenolic acid contents including gallic acid, chlorogenic acid, catechin, ρ-coumaric acid, ferulic acid, salicylic acid, naringin, myricetin, trans-cinnamic acid, naringenin and kaempferol increased from 37.26~204.32 μg/g at HP0 to 77.29~283.05 μg/g at HP48. In anti-oxidant activity analyses, HP48 extracts showed higher values in ABTS and DPPH radical scavenging activity, reducing power, and Fe2+ iron chelating effect than those of the HP24 and HP0 extracts. These results suggest that the combined treatment of high pressure treatment and germination process efficiently enhanced antioxidant compound and activity of rough rice.


Lee S.H.,Chungbuk National University | Jang G.Y.,Chungbuk National University | Kim M.Y.,Chungbuk National University | Kim S.,Genome Corp. | And 3 more authors.
Journal of the Korean Society of Food Science and Nutrition | Year: 2015

This study was performed to investigate the changes in monacolin K content and antioxidant activities of Monascus-fermented brown rice with different germination temperatures and periods. Brown rice was germinated at 32, 35 and 37°C for 1∼4 days, after white rice (WB), brown rice (BR), and germinated brown rice (GBR) were fermented with M. pilosus 305-9 at 30°C for 20 days. The redness, yellowness and Monascus pigments increased after germination. Total monacolin K content increased from 215.85 mg/kg of BR to 1,263.04 mg/kg of GBR (32°C/1 day), whereas monacolin K content decreased with increase in germination period. Citrinin was not detected in any of the samples. Total polyphenol (TPC) and flavonoid contents (TFC) increased with increase in germination temperature and period, whereas electron donating ability (EDA) and total antioxidant activities (TAA) decreased due to reduction of Monascus pigment content. The TPC and TFC showed the highest values (13.80 mg/g and 1.30 mg/g, respectively) in GBR (37°C/4 day), whereas EDA and TAA showed the highest values (22.16 mg Trolox equivalent/g and 62.27 mg ascorbic acid equivalent/g, respectively) in GBR (32°C/1 day). These results indicated that the optimal germination temperature and period for increasing monacolin K content and antioxidant activities was found to be at 32°C for 1 day. In addition, it was found that M. pilosus 305-9 was a useful strain for increasing monacolin K content without producing citrinin in functional foods and pharmaceutical industrial regions. © 2015, Korean Society of Food Science and Nutrition. All rights reserved.


Kim M.Y.,Chungbuk National University | Lee S.H.,Chungbuk National University | Jang G.Y.,Chungbuk National University | Park H.J.,Chungbuk National University | And 5 more authors.
Korean Journal of Food Science and Technology | Year: 2014

We evaluated the enzyme inhibitory activity of germinated rough rice (Oryza sativar L.) treated by high pressure (30MPa) for 24 h (HP24) and 48 h (HP48). In rice germinated for 1 day, the a-glucosidase inhibitory activity reached its highest level, 68.32%, at HP48. The a-amylase inhibitory activity increased from 32.66-57.00% at HP0, to 43.67-74.82% at HP48. On the other hand, the inhibitory activity of angiotensin-converting enzyme increased from 27.98% to 49.42% over the course of the second day of HP48. The inhibitory activity of xanthine oxidase peaked of 67.51% at HP48 and subsequently decreased. Lipase inhibitory activity increased from 24.04-47.91% at HP0, to 29.62-64.63% at HP48. These results provide useful information for the use of germinated rough rice as a functional food material and demonstrate that high-pressure treatment during the germination process efficiently increase enzyme inhibitory activity.©The Korean Society of Food Science and Technology 2014.


PubMed | Genome Corp.
Type: | Journal: BMC plant biology | Year: 2010

Root and bulb vegetables (RBV) include carrots, celeriac (root celery), parsnips (Apiaceae), onions, garlic, and leek (Alliaceae)--food crops grown globally and consumed worldwide. Few data analysis platforms are currently available where data collection, annotation and integration initiatives are focused on RBV plant groups. Scientists working on RBV include breeders, geneticists, taxonomists, plant pathologists, and plant physiologists who use genomic data for a wide range of activities including the development of molecular genetic maps, delineation of taxonomic relationships, and investigation of molecular aspects of gene expression in biochemical pathways and disease responses. With genomic data coming from such diverse areas of plant science, availability of a community resource focused on these RBV data types would be of great interest to this scientific community.The RoBuST database has been developed to initiate a platform for collecting and organizing genomic information useful for RBV researchers. The current release of RoBuST contains genomics data for 294 Alliaceae and 816 Apiaceae plant species and has the following features: (1) comprehensive sequence annotations of 3663 genes 5959 RNAs, 22,723 ESTs and 11,438 regulatory sequence elements from Apiaceae and Alliaceae plant families; (2) graphical tools for visualization and analysis of sequence data; (3) access to traits, biosynthetic pathways, genetic linkage maps and molecular taxonomy data associated with Alliaceae and Apiaceae plants; and (4) comprehensive plant splice signal repository of 659,369 splice signals collected from 6015 plant species for comparative analysis of plant splicing patterns.RoBuST, available at http://robust.genome.com, provides an integrated platform for researchers to effortlessly explore and analyze genomic data associated with root and bulb vegetables.

Loading Genome Corp. collaborators
Loading Genome Corp. collaborators