Ikeda S.S.,Red Cross |
Kobayashi M.,Health Sciences University of Hokkaido |
Osaki M.,Tottori University |
Shikanai M.,Genmai Koso Co. |
And 2 more authors.
Nutrients | Year: 2015
We have established an inflammation-related carcinogenesis model in mouse, in which regressive QR-32 cells subcutaneously co-implanted with a foreign body—gelatin sponge—convert themselves into lethal tumors due to massive infiltration of inflammatory cells into the sponge. Animals were fed with a diet containing 5% or 10% fermented brown rice and rice bran with Aspergillus oryzae (FBRA). In 5% and 10% FBRA diet groups, tumor incidences were lower (35% and 20%, respectively) than in the non-treated group (70%). We found that FBRA reduced the number of inflammatory cells infiltrating into the sponge. FBRA administration did not cause myelosuppression, which indicated that the anti-inflammatory effects of FBRA took place at the inflammatory lesion. FBRA did not have antitumor effects on the implanted QRsP-11 tumor cells, which is a tumorigenic cell line established from a tumor arisen after co-implantation of QR-32 cells with sponge. FBRA did not reduce formation of 8-hydroxy-2′-deoxyguanine adducts, a marker of oxidative DNA damage in the inflammatory lesion; however, it reduced expression of inflammation-related genes such as TNF-α, Mac-1, CCL3 and CXCL2. These results suggest that FBRA will be an effective chemopreventive agent against inflammation-related carcinogenesis that acts by inhibiting inflammatory cell infiltration into inflammatory lesions. © 2015 by the authors; licensee MDPI, Basel, Switzerland.
Nemoto H.,Tokushima University |
Nemoto H.,Genmai Koso Co. |
Kataoka K.,Tokushima University |
Ishikawa H.,Tokushima University |
And 7 more authors.
Digestive Diseases and Sciences | Year: 2012
Background: Clinical observations and experimental colitis models have indicated the importance of intestinal bacteria in the etiology of ulcerative colitis (UC), but a causative bacterial agent has not been identified. Aim: To determine how intestinal bacteria are associated with UC, fecal microbiota and other components were compared for UC patients and healthy adults. Methods: Fresh feces were collected from 48 UC patients. Fecal microbiota were analyzed by use of terminalrestriction fragment length polymorphism (T-RFLP), realtime PCR, and culture. The concentrations of organic acids, indole, and ammonia, and pH and moisture, which are indicators of the intestinal environment, were measured and compared with healthy control data. Results: T-RFLP data divided the UC patients into four clusters; one cluster was obtained for healthy subjects. The diversity of fecal microbiota was significantly lower in UC patients. There were significantly fewer Bacteroides and Clostridium subcluster XIVab, and the amount of Enterococcus was higher in UC patients than in healthy subjects. The fecal concentration of organic acids was significantly lower in UC patients who were in remission. Conclusion: UC patients have imbalances in the intestinal environment-less diversity of fecal microbiota, lower levels of major anaerobic bacteria (Bacteroides and Clostridium subcluster XIVab), and a lower concentration of organic acids. © Springer Science+Business Media, LLC 2012.
Phutthaphadoong S.,Gifu University |
Phutthaphadoong S.,Chiang Mai University |
Yamada Y.,Gifu University |
Hirata A.,Gifu University |
And 6 more authors.
Oncology Reports | Year: 2010
Our previous study revealed that fermented brown rice and rice bran (FBRA) suppresses rat colorectal carcinogenesis induced by azoxymethane, the colon-specific carcinogen. In the present study, we examined the suppressive effect of FBRA on colon carcinogenesis in ApcMin/+ mouse, a mouse model for human familial adenomatous polyposis. In contrast to previous findings with the carcinogen-induced model, administration of 5 and 10% FBRA had no effect on the tumor development in the colon of ApcMin/+ mice, suggesting that the modifying effects of FBRA on colorectal carcinogenesis are different depending on rodent models for colorectal carcinogenesis. However, when FBRA is administrated in dextran sodium sulfate (DSS)-exposed Apc Min/+ mouse, a mouse model for the inflammation-related colorectal carcinogenesis, FBRA significantly suppressed the multiplicity of colon tumors in comparison with control diet group. FBRA administration suppressed the cell proliferative index, which is accompanied by the significantly decreased mRNA expressions of Cox2 and iNos in colonic mucosa exposed to DSS (p<0.04 and 0.02, respectively). These findings indicate that FBRA has chemopreventive effects specifically against inflammation-related tumorigenesis in the colon. Our findings also suggest that anti-inflammatory activity is one of the underlying mechanisms by which FBRA suppresses tumorigenesis in the colon.
Nemoto H.,Tokushima University |
Nemoto H.,Genmai Koso Co. |
Ikata K.,Tokushima University |
Arimochi H.,Tokushima University |
And 4 more authors.
Journal of Medical Investigation | Year: 2011
Purpose: The aim of this study is to investigate the prebiotic effects of brown rice fermented by Aspergillus oryzae (FBRA) on the intestinal environment in vitro and in healthy adults. Methods: Fresh fecal slurries from six healthy adults were incubated with FBRA to confirm prebiotic potentials of FBRA. Another thirty-six healthy adults were randomly allocated to 2 groups for the clinical study. Subjects consumed 21.0 g/day of either FBRA or control food for 2 weeks, followed by a 12-week intermission and then 2-week ingestion vice versa. Main outcome measures were bifidobacterial numbers and organic acid concentration in feces. Sub outcome measures were fecal microbiota, fecal environments and bowel function. Results: Incubation of fecal slurries with FBRA in vitro resulted in increased organic acids with individual-specific patterns. Bifidobacterial numbers were increased during incubation. In the clinical study, all participants safely completed this study. FBRA had little effect on fecal number of bifidobacteria, concentrations of organic acids or putrefactive metabolites, fecal pH, or fecal microbiota. Conclusion: FBRA has the potentials as a prebiotic, however, we could not detect its effects on the intestinal environment in vivo. The results in a clinical study indicated that FBRA could be safely used for healthy adults.