Hatfield, PA, United States
Hatfield, PA, United States

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Patent
Genisphere Inc | Date: 2013-07-12

The present invention relates to lateral flow devices and kits using DNA dendrimers, and to methods for detecting an analyte using the lateral flow devices and kits. More specifically, the DNA dendrimers may be dried on a location on the test device upstream from said test zone, before said test device is used.


Methods, reagents and kits are provided for the production and use in detection assays of labeled nucleic acid molecules wherein a labeling molecule is attached directly to the 3 end of the nucleic acid molecules.


Patent
Genisphere Inc and Affymetrix | Date: 2012-06-18

Methods for diagnosis and surveillance of complex multi-factorial disorders such as cancer by screening of easily accessible biomarkers are disclosed. Highly stable cell free Circulating Nucleic Acids (CNA) present as both RNA and DNA species have been discovered in the blood and plasma of humans. Correlations between tumor-associated genomic/epigenetic/transcriptional changes and alterations in CNA levels are strong predictors of the utility of this biomarker class as clinical indicators. Methods for using microRNAs (miRNAs) representing a class of naturally occurring small non-coding RNAs of 19-25 nt as markers that can associate their specific expression profiles with cancer development are disclosed. Methods for isolating plasma fractions for the study of miRNA biomarkers and for measurement of circulating miRNA levels are disclosed.


Patent
Genisphere Inc | Date: 2014-03-19

Compositions and methods for targeted delivery of active agents to cells are provided. The compositions comprise a wholly or partially double-stranded synthetic DNA carrier, and an active agent intercalated in double-stranded portions of the DNA carrier. The DNA carrier may also be linked to a targeting agent. The compositions are useful for delivering an active agent into a targeted cell type, for example a cytotoxic agent.


Patent
Genisphere Inc | Date: 2013-03-15

The present invention relates to novel lateral flow devices using DNA dendrimers, and the methods for detecting an analyte using the lateral flow devices.


The invention provides methods for protecting molecules from degradation in a bodily fluid like serum. In a first aspect, the method is for protecting a DNA dendrimer from degradation, and comprises the step of attaching the DNA dendrimer to a protein, digoxigenin, cholesterol, a primary amine, a hydrocarbon spacer of length of 3 to 120 carbons, a PEG molecule, biotin, a biotin derivative, fluorescein or a fluorescein derivative, or any combination thereof; thereby protecting the DNA dendrimer against nuclease degradation. In a second aspect, the method is for protecting a siRNA from degradation, comprising attaching the siRNA to a protected DNA dendrimer obtained in the first aspect; thereby protecting the siRNA against degradation.


Patent
Genisphere Inc and Lankenau Institute Of Medical Research | Date: 2014-01-31

Compositions are disclosed which comprise a DNA dendrimer having one or more DNA sequences linked thereto, the DNA sequences comprising DNA sequences encoding a polypeptide or regulatory RNA linked to DNA sequences that regulate expression of the DNA sequences encoding a polypeptide or regulatory RNA to produce an RNA coding for the polypeptide or to produce the regulatory RNA. Also disclosed are methods for treating diseases and conditions of cells by delivering the dendrimers to the cells, with subsequent expression of the encoded polypeptide or regulatory RNA.


Patent
Genisphere Inc | Date: 2013-11-08

Methods for preparing strand-specific sequencing libraries of oligonucleotides using an RNA polymerase promoter to re-transcribe antisense cDNA which has been reverse transcribed from mRNA are provided. The transcription step linearly amplifies sRNA prior to production of double-stranded cDNA to be sequenced and may be sufficient to eliminate the conventional PCR amplification step prior to sequencing. The methods incorporate anchor sequences, amplification sequences and other sequences required for a particular sequencing system or reaction by hybridization and extension of primers, and transcription of RNA, rather than ligation, thus reducing the number of steps and the time required for sample preparation for sequencing of RNA. Use of primer hybridization and transcription reactions in the methods also results in a library that exhibits reduced 3 sequence bias.


Patent
Genisphere Inc | Date: 2011-09-20

Methods for diagnosing inflammatory bowel disease and ulcerative colitis using miRNA biomarkers for these diseases are provided. Differential expression of the miRNA biomarkers in blood fractions, e.g., platelets, of diseased individuals as compared to expression levels in normal individuals indicates the presence of IBD or ulcerative colitis. Also provided are microarrays for use in the diagnostic methods, wherein the features of the microarray consist essentially of nucleic acid sequences that hybridize to the miRNA biomarkers and normalization controls.


Patent
Genisphere Inc | Date: 2013-11-06

DNA dendrimers for targeted delivery of radiation absorbing nanoparticles and thermal ablation of cells and tissues are provided. Also provided are methods of making and methods of using the DNA dendrimers.

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