El Amri H.,Genetics Laboratory of Royal Gendarmery |
Squalli D.,GENOME Biothechnologie |
Gazzaz B.,Genetics Laboratory of Royal Gendarmery |
El Harrak Hajri A.,Genetics Laboratory of Royal Gendarmery |
El Ossmani H.,Genetics Laboratory of Royal Gendarmery
Forensic Science International: Genetics Supplement Series | Year: 2011
Polymerase chain reaction (PCR) amplification using the AmpFℓSTR® NGM™ Kit (" NGM," an acronym for "Next Generation Multiplex") was performed in random sample of 180 unrelated individuals from three Moroccan population groups (Arab Speaking, Berber Speaking, and Sahrawi). Allele frequency and other forensically relevant statistics data were generated for the NGM™ multiplex kit includes the original 10STR loci from the AmpFℓSTR SGM Plus® kit (D3S1358, vWA, D16S539, D2S1338, D8S1179, D19S433, TH01, FGA, D21S11, D18S51) together with five additional STRs (D10S1248, D22S1045, D2S441, D1S1656 and D12S391) and the Amelogenin sex-determination locus. Population study was conducted to evaluate usefulness of the loci (especially the five new microsatellite systems) in forensic genetic identification examinations. All Fifteen autosomal STR loci were found to be in Hardy-Weinberg equilibrium. Discrimination power was particularly high in case of D18S51 and D12S391 STR loci. © 2011 Elsevier Ireland Ltd.