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Lelu-Walter M.-A.,French National Institute for Agricultural Research | Thompson D.,Coillte Teoranta The Irish Forestry Board | Harvengt L.,Genetics and Biotechnology Laboratory | Sanchez L.,French National Institute for Agricultural Research | And 2 more authors.
Tree Genetics and Genomes | Year: 2013

Vegetative propagation of forest trees offers advantages to both tree breeders and the forest industry. This review will describe benefits, type of vegetative propagation, and its integration into breeding programmes. Of all of the different methods for vegetative propagation, only rooted cuttings and somatic embryogenesis (and the combined use of both) offer any practical methods for large-scale commercial use. However, it is very difficult to fully appreciate the overall level of activity of the research and application of somatic embryogenesis of forest trees. Publications and reports only highlight a small fraction of the ongoing work. To this end, a survey was conducted across Europe (under EU Research Infrastructure Concerted Action "Treebreedex") to document the species involved, the state-of-the-art of somatic embryogenesis, its stage of development and its application in tree improvement programmes and to commercial forestry. The results of this survey are presented and discussed. In addition, this review presents the challenges (biological, economic, public acceptance and regulatory) and their relationships to European forestry. Finally, a strategy to promote the use of this technology is proposed. © 2013 Springer-Verlag Berlin Heidelberg. Source

Rodrigues F.A.,Brazilian Agricultural Research Corporation Embrapa Soybean | Fuganti-Pagliarini R.,Brazilian Agricultural Research Corporation Embrapa Soybean | Marcolino-Gomes J.,Brazilian Agricultural Research Corporation Embrapa Soybean | Marcolino-Gomes J.,State University Londrina | And 9 more authors.
BMC Genomics | Year: 2015

Background: Since drought can seriously affect plant growth and development and little is known about how the oscillations of gene expression during the drought stress-acclimation response in soybean is affected, we applied Illumina technology to sequence 36 cDNA libraries synthesized from control and drought-stressed soybean plants to verify the dynamic changes in gene expression during a 24-h time course. Cycling variables were measured from the expression data to determine the putative circadian rhythm regulation of gene expression. Results: We identified 4866 genes differentially expressed in soybean plants in response to water deficit. Of these genes, 3715 were differentially expressed during the light period, from which approximately 9.55 % were observed in both light and darkness. We found 887 genes that were either up- or down-regulated in different periods of the day. Of 54,175 predicted soybean genes, 35.52 % exhibited expression oscillations in a 24 h period. This number increased to 39.23 % when plants were submitted to water deficit. Major differences in gene expression were observed in the control plants from late day (ZT16) until predawn (ZT20) periods, indicating that gene expression oscillates during the course of 24 h in normal development. Under water deficit, dissimilarity increased in all time-periods, indicating that the applied stress influenced gene expression. Such differences in plants under stress were primarily observed in ZT0 (early morning) to ZT8 (late day) and also from ZT4 to ZT12. Stress-related pathways were triggered in response to water deficit primarily during midday, when more genes were up-regulated compared to early morning. Additionally, genes known to be involved in secondary metabolism and hormone signaling were also expressed in the dark period. Conclusions: Gene expression networks can be dynamically shaped to acclimate plant metabolism under environmental stressful conditions. We have identified putative cycling genes that are expressed in soybean leaves under normal developmental conditions and genes whose expression oscillates under conditions of water deficit. These results suggest that time of day, as well as light and temperature oscillations that occur considerably affect the regulation of water deficit stress response in soybean plants. © 2015 Rodrigues et al. Source

Catao E.C.P.,University of Brasilia | Lopes F.A.C.,University of Brasilia | Araujo J.F.,University of Brasilia | De Castro A.P.,University of Brasilia | And 6 more authors.
International Journal of Microbiology | Year: 2014

16S rRNA sequences from the phylum Acidobacteria have been commonly reported from soil microbial communities, including those from the Brazilian Savanna (Cerrado) and the Atlantic Forest biomes, two biomes that present contrasting characteristics of soil and vegetation. Using 16S rRNA sequences, the present work aimed to study acidobacterial diversity and distribution in soils of Cerrado savanna and two Atlantic forest sites. PCA and phylogenetic reconstruction showed that the acidobacterial communities found in "Mata de galeria" forest soil samples from the Cerrado biome have a tendency to separate from the other Cerrado vegetation microbial communities in the direction of those found in the Atlantic Forest, which is correlated with a high abundance of Acidobacteria subgroup 2 (GP2). Environmental conditions seem to promote a negative correlation between GP2 and subgroup 1 (GP1) abundance. Also GP2 is negatively correlated to pH, but positively correlated to high Al3+ concentrations. The Cerrado soil showed the lowest Acidobacteria richness and diversity indexes of OTUs at the species and subgroups levels when compared to Atlantic Forest soils. These results suggest specificity of acidobacterial subgroups to soils of different biomes and are a starting point to understand their ecological roles, a topic that needs to be further explored. © 2014 Elisa C. P. Catão et al. Source

Alves A.A.,Genetics and Biotechnology Laboratory | Laviola B.G.,Genetics and Biotechnology Laboratory | Formighieri E.F.,Genetics and Biotechnology Laboratory | Carels N.,Center for Technological Development in Health
Biotechnology Journal | Year: 2015

Genomics provides the opportunity to explore the relationships of genes and phenotypes: its operational use in the context of breeding programs through strategies such as genomic selection, promises to foster the development of perennial crops dedicated to biodiesel production by increasing the efficiency of breeding programs and by shortening the length of the breeding cycles. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Source

Martins P.K.,Genetics and Biotechnology Laboratory | Nakayama T.J.,Federal University of Vicosa | Nakayama T.J.,Plant Biotechnology Laboratory | Ribeiro A.P.,Genetics and Biotechnology Laboratory | And 6 more authors.
Biotechnology Reports | Year: 2015

Abstract Setaria viridis was recently described as a new monocotyledonous model species for C4 photosynthesis research and genetic transformation. It has biological attributes (rapid life cycle, small genome, diploid, short stature and simple growth requirements) that make it suitable for use as a model plant. We report an alternative method of S. viridis transformation using floral dip to circumvent the necessity of tissue culture phase for transgenic plant regeneration. S. viridis spikes at boot stage were selected to be immersed in Agrobacterium suspension. T1 seeds could be identified in 1.5-2 months after floral dipping. We demonstrated through molecular analysis and RFP expression that seeds and resulting plants from dipped inflorescences were transformed. Our results suggest the feasibility of S. viridis floral dip transformation as a time-saving and cost-effective compared with traditional methods. To our knowledge, this is the first report using floral dip in S. viridis as an Agrobacterium-mediated transformation method. © 2015 The Author. Source

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