Entity

Time filter

Source Type


Esmailnia E.,Genetics and Agricultural Biotechnology Institute of Tabarestan GABIT | Arefrad M.,Genetics and Agricultural Biotechnology Institute of Tabarestan GABIT | Shabani S.,Genetics and Agricultural Biotechnology Institute of Tabarestan GABIT | Karimi M.,Genetics and Agricultural Biotechnology Institute of Tabarestan GABIT | And 2 more authors.
Biharean Biologist | Year: 2015

Inter Simple Sequence Repeat (ISSR) markers were employed to study the genetic diversity among 30 indigenous species of Iranian cucurbit from five different genera of Cucurbitaceae. Eleven out of seventeen studied primers amplified a total of 283 bands, out of which 263 (92.93%) were polymorphic. The mean Polymorphism Information Content (PIC) was estimated at 0.327. Among the primers, ISSR15 exhibited the highest polymorphic bands and PIC value and was recognized as the most appropriate and discriminating primer to investigate genetic diversity. The results of clustering analysis showed that the least distance was observed between two species of Cucumis L. genus from Mashhad and Sabzevar with a genetic similarity value of 68.34%, while the highest genetic distance was observed between two species of Cucumis L. and Cucurbita L. genera from Dastgerd and Hamadan with a genetic similarity value of 20.71%. Although Cucurbita maxima and Cucurbita maschata were genetically similar, they weren't classified in the same cluster. These results indicated an extensive genetic variation within Iranian Cucumis L. and Cucurbita L. germplasm. The high genetic variability among studied species would be beneficial for selection of a core collection to facilitate germplasm management to be used in cucurbit breeding and conservation programs. The results of the present study confirmed that fingerprinting of cucurbits genotypes for identification purposes could be achieved by the ISSR technique. © Biharean Biologist, Oradea, Romania, 2015. Source

Discover hidden collaborations