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Zhang H.,Genetic Engineering Laboratory | Zhao W.-J.,Genetic Engineering Laboratory | Kang L.C.,Genetic Engineering Laboratory | Wang X.-H.,Genetic Engineering Laboratory | Bo X.-W.,Genetic Engineering Laboratory
Molecular Biology Reports | Year: 2010

Ubiquitin and other ubiquitin-like proteins play important roles in post-translational modification. They are phylogenetically well-conserved in eukaryotes. Here we report a new ubiquitin-conjugating enzyme E2 cDNA, which containing a ubiquitin-conjugating enzyme UBCcdomain named UBE2AM. Its cDNA is 899 base pairs in length and contains an open reading frame from nucleotide 171 to 632 encoding 153 amino acids. The result of real time RT-PCR showed that UBEA2 M is expressed in most of M. expansa proglottides and over-expressed in the mature proglottides. Comparison of predicted UBE2AM with UBCc (protein) homologues/orthologous from other speciesrevealed identities between species varying from 97.5 to 99.4% at the amino acid level. Phylogenetic analysis showed the UBE2AM is a member of the eukaryotic UBCc superfamily, which have diverged from a common ancestor and the gene is clustered in the same group with the ubiquitinconjugating enzyme E2A-like protein from Taenia asiatica. © Springer Science+Business Media B.V. 2009.


PubMed | Genetic Engineering Laboratory
Type: Journal Article | Journal: Molecular biology reports | Year: 2010

Ubiquitin and other ubiquitin-like proteins play important roles in post-translational modification. They are phylogenetically well-conserved in eukaryotes. Here we report a new ubiquitin-conjugating enzyme E2 cDNA, which containing a ubiquitin-conjugating enzyme UBCc-domain named UBE2AM. Its cDNA is 899 base pairs in length and contains an open reading frame from nucleotide 171 to 632 encoding 153 amino acids. The result of real time RT-PCR showed that UBEA2 M is expressed in most of M. expansa proglottides and over-expressed in the mature proglottides. Comparison of predicted UBE2AM with UBCc (protein) homologues/orthologous from other species revealed identities between species varying from 97.5 to 99.4% at the amino acid level. Phylogenetic analysis showed the UBE2AM is a member of the eukaryotic UBCc superfamily, which have diverged from a common ancestor and the gene is clustered in the same group with the ubiquitin-conjugating enzyme E2A-like protein from Taenia asiatica.


PubMed | Genetic Engineering Laboratory
Type: Journal Article | Journal: Behavioral neuroscience | Year: 2015

The estradiol (E2), estrogen receptor- (ER), testosterone (T), and androgen receptor (AR) can contribute to anxiety, but whether they are associated with the reversion of prenatal adverse outcomes remains unclear. Here, we tested the interactive effects of prenatal maternal restraint stress and early postnatal short-term maternal separation on adult male mandarin vole (Microtus mandarinus) behavior and changes in E2, T, and their receptors. The results showed that PS adult males (PS/NH) exhibited an increase in anxiety-like behavior in open-field and elevated plus-maze tests than the other 3 groups, including adult male offspring controls (PC/NH), adult male offspring controls with short-term maternal separation (PC/H), and PS adult males with short-term maternal separation (PS/H). The increase in anxiety-like behavior was associated with significantly lower E2 and T serum levels, had significantly more ER immunoreactive neurons (ER-IRs) in some brain regions, as well as significantly fewer AR immunoreactive neurons (AR-IRs) in some brain regions than the other 3 groups. We found it interesting that the PC/H and PS/H were similar to the PC/NH in that they did not produce anxiety-like behavior. However, early postnatal short-term maternal separation reversed prenatally induced changes in E2 and T serum levels and altered ER-IRs and AR-IRs in the brain. These data suggest that changes in anxious adults may be governed by early environmental factors and their interactions because changes in E2 and T serum levels and the distribution of ER and AR in the brain result in behavioral changes related to less anxiety into adulthood.

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